Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Ascaris suum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NAD+ | native enzyme | Ascaris suum | |
0.015 | - |
NAD+ | recombinant enzyme | Ascaris suum | |
1 | - |
L-malate | native enzyme | Ascaris suum | |
1.1 | - |
L-malate | recombinant enzyme | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ascaris suum | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Ascaris suum | - |
CO2 + pyruvate + NADH | - |
? | |
Oxaloacetate | Ascaris suum | - |
CO2 + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate ammonium sulfate, Phenyl-sepharose, Orange-A-Sepharose | Ascaris suum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.5 | - |
recombinant enzyme | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Ascaris suum | CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NAD+ | - |
Ascaris suum | CO2 + pyruvate + NADH | - |
r | |
Oxaloacetate | - |
Ascaris suum | CO2 + pyruvate | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
L-malate | native and recombinant enzyme | Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ascaris suum |