1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating), go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

malic enzyme, MHD, mitochondrial malic enzyme ME2, mitochondrial NAD(P) malic enzyme ME2, mitochondrial NAD-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent ME, NAD-malic enzyme, NAD-ME, NAD-specific malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.38 malate dehydrogenase (oxaloacetate-decarboxylating)

Engineering

Engineering on EC 1.1.1.38 - malate dehydrogenase (oxaloacetate-decarboxylating)

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N434A
with N434A, the interaction of the residue with malate is lost, causing the malate to reorient itself, leading to a slower decarboxylation step
N479Q
the Kmalate value for the N479Q mutant enzyme increases by 2.2fold compared to the wild type enzyme, the KNAD value for the N479Q mutant enzyme increases by 1.1fold compared to the wild type enzyme
N479S
the Kmalate value for the N479Q mutant enzyme increases by 2.1fold compared to the wild type enzyme, the KNAD value for the N479Q mutant enzyme increases by 1.8fold compared to the wild type enzyme
S433A
the KNAD value for the S433A mutant enzyme increases by 80fold compared to the wild type enzyme, indicating that this residue provides significant binding affinity for the dinucleotide
G444A
-
inactive enzyme