Cloned (Comment) | Organism |
---|---|
gene ME2, quantitative RT-PCR expression analysis of isozymes Me1, Me2, and Me3 in wild-type strain and mutant Me2 strains | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Me2 single and double knockdown cell lines, thereby generating cel lines Me22124(H)/Me22124(P) and Me22124(H)/Me2-654(P), overview. Me2 mRNA and ME2 enzyme activity are drastically reduced in the double-knockdown cell lines Me2-2124(H)/Me2-2124(P) and Me2-2124(H)/Me2-456(P). Me3 mRNA is slightly reduced in these two cell lines, whereas Me1 mRNA and ME1 enzyme activity are not affected. Resident siRNA might interfere with the expression of the siRNA expressed from the second vector. Knockdown of isozyme ME3, EC 1.1.1.40, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Rattus norvegicus | - |
pyruvate + CO2 + NADH | - |
? | |
(S)-malate + NADP+ | Rattus norvegicus | - |
pyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | A0A0G2K502 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
insulinoma cell | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rattus norvegicus | pyruvate + CO2 + NADH | - |
? | |
(S)-malate + NADP+ | - |
Rattus norvegicus | pyruvate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ME2 | - |
Rattus norvegicus |
mitochondrial NAD(P) malic enzyme | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of ME2 does not inhibit insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine | Rattus norvegicus |