1.1.1.169: 2-dehydropantoate 2-reductase

This is an abbreviated version, for detailed information about 2-dehydropantoate 2-reductase, go to the full flat file.

Reaction

(R)-pantoate
+
NADP+
=
2-dehydropantoate
+
NADPH
+
H+

Synonyms

2-dehydropantoate 2-reductase, 2-ketopantoate reductase, 2-ketopantoic acid reductase, 2-oxopantoate reductase, ketopantoate reductase, ketopantoic acid reductase, KPA reductase, KPR, More, panG, Tk-KPR, TK1968, TK1968 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.169 2-dehydropantoate 2-reductase

Purification

Purification on EC 1.1.1.169 - 2-dehydropantoate 2-reductase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anion-exchange chromatography, gel filtration chromatography
-
FPLC, anion exchange chromatography, gel filtration
gel chromatography
homogeneity in a yield of 20-60 mg from 3-6 g of cells
-
native wild-type enzyme by anion exchange and adsorption chromatography, and gel filtration, recombinant His6-tagged enzyme by nickel affinity chromatography
-
on nickel-nitrilotriacetic acid resin and by gel filtration, more than 98% pure
recombinant C-terminally His-tagged wild-type and mutant enzymes; TALON affinity resin column chromatography
recombinant enzyme from Escherichia coli by heat treatment, anion-exchange chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta2 (DE3) pLysS by nickel affinity chromatography, avidin affinity chromatography, and dialysis
-
recombinant wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer from Escherichia coli by affinity chromatography
-