BRENDA - Enzyme Database show
show all sequences of 1.1.1.169

ApbA, the ketopantoate reductase enzyme of Salmonella typhimurium is required for the synthesis of thiamine via the alternative pyrimidine biosynthetic pathway

Frodyma, M.E.; Downs, D.; J. Biol. Chem. 273, 5572-5576 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21/lambdaDE3
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.742
-
ketopantoic acid
+/-0.01
Salmonella enterica subsp. enterica serovar Typhimurium
0.776
-
NADPH
+/-0.09
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31100
-
HPLC gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ketopantoate + NADPH
Escherichia coli
-
pantoate + NADP+
-
Escherichia coli
?
ketopantoate + NADPH
Salmonella enterica subsp. enterica serovar Typhimurium
thiamine synthesis, pantothenate and thiamine biosynthetic pathway
pantoate + NADP+
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoate + NADPH
Escherichia coli BL21/lambdaDE3
-
pantoate + NADP+
-
Escherichia coli BL21/lambdaDE3
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
BL21/lambdaDE3
-
Escherichia coli BL21/lambdaDE3
-
BL21/lambdaDE3
-
Saccharomyces cerevisiae
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Stenotrophomonas maltophilia
-
845
-
Stenotrophomonas maltophilia 845
-
845
-
Purification (Commentary)
Commentary
Organism
chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Saccharomyces cerevisiae
additional information
-
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
16
-
optimal reaction conditions using NADPH
Salmonella enterica subsp. enterica serovar Typhimurium
61
-
optimal reaction conditions using NADH
Salmonella enterica subsp. enterica serovar Typhimurium
64.3
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ketopantoate + NADPH
-
286107
Escherichia coli
pantoate + NADP+
-
286107
Escherichia coli
?
ketopantoate + NADPH
thiamine synthesis, pantothenate and thiamine biosynthetic pathway
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoate + NADP+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoate + NADPH
-
286107
Escherichia coli BL21/lambdaDE3
pantoate + NADP+
-
286107
Escherichia coli BL21/lambdaDE3
?
ketopantoic acid + NADH
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoic acid + NAD+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoic acid + NADPH
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoic acid + NADP+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoic acid + NADPH
-
286107
Escherichia coli
pantoic acid + NADP+
-
286107
Escherichia coli
?
ketopantoic acid + NADPH
-
286107
Saccharomyces cerevisiae
pantoic acid + NADP+
-
286107
Saccharomyces cerevisiae
?
ketopantoic acid + NADPH
-
286107
Escherichia coli BL21/lambdaDE3
pantoic acid + NADP+
-
286107
Escherichia coli BL21/lambdaDE3
?
additional information
-
286107
Escherichia coli
?
-
-
-
-
additional information
-
286107
Saccharomyces cerevisiae
?
-
-
-
-
additional information
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
-
286107
Escherichia coli BL21/lambdaDE3
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
-
Escherichia coli
monomer
1 * 31100, HPLC gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
reduction of ketopantoic acid at pH 6.0
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
reduction of pantoic acid at 42C
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
in presence of NADPH the ketopantoic acid reductase does not reduce artificial dye electron acceptors
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
-
Stenotrophomonas maltophilia
NADH
-
Saccharomyces cerevisiae
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Saccharomyces cerevisiae
NADPH
preferred
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Stenotrophomonas maltophilia
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21/lambdaDE3
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
in presence of NADPH the ketopantoic acid reductase does not reduce artificial dye electron acceptors
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
-
Stenotrophomonas maltophilia
NADH
-
Saccharomyces cerevisiae
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Saccharomyces cerevisiae
NADPH
preferred
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Stenotrophomonas maltophilia
NADPH
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.742
-
ketopantoic acid
+/-0.01
Salmonella enterica subsp. enterica serovar Typhimurium
0.776
-
NADPH
+/-0.09
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31100
-
HPLC gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ketopantoate + NADPH
Escherichia coli
-
pantoate + NADP+
-
Escherichia coli
?
ketopantoate + NADPH
Salmonella enterica subsp. enterica serovar Typhimurium
thiamine synthesis, pantothenate and thiamine biosynthetic pathway
pantoate + NADP+
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoate + NADPH
Escherichia coli BL21/lambdaDE3
-
pantoate + NADP+
-
Escherichia coli BL21/lambdaDE3
?
Purification (Commentary) (protein specific)
Commentary
Organism
chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Saccharomyces cerevisiae
additional information
-
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
16
-
optimal reaction conditions using NADPH
Salmonella enterica subsp. enterica serovar Typhimurium
61
-
optimal reaction conditions using NADH
Salmonella enterica subsp. enterica serovar Typhimurium
64.3
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ketopantoate + NADPH
-
286107
Escherichia coli
pantoate + NADP+
-
286107
Escherichia coli
?
ketopantoate + NADPH
thiamine synthesis, pantothenate and thiamine biosynthetic pathway
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoate + NADP+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoate + NADPH
-
286107
Escherichia coli BL21/lambdaDE3
pantoate + NADP+
-
286107
Escherichia coli BL21/lambdaDE3
?
ketopantoic acid + NADH
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoic acid + NAD+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoic acid + NADPH
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
pantoic acid + NADP+
-
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
ketopantoic acid + NADPH
-
286107
Escherichia coli
pantoic acid + NADP+
-
286107
Escherichia coli
?
ketopantoic acid + NADPH
-
286107
Saccharomyces cerevisiae
pantoic acid + NADP+
-
286107
Saccharomyces cerevisiae
?
ketopantoic acid + NADPH
-
286107
Escherichia coli BL21/lambdaDE3
pantoic acid + NADP+
-
286107
Escherichia coli BL21/lambdaDE3
?
additional information
-
286107
Escherichia coli
?
-
-
-
-
additional information
-
286107
Saccharomyces cerevisiae
?
-
-
-
-
additional information
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM
286107
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
-
286107
Escherichia coli BL21/lambdaDE3
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Escherichia coli
monomer
1 * 31100, HPLC gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
reduction of ketopantoic acid at pH 6.0
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
reduction of pantoic acid at 42C
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 1.1.1.169
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725293
Miller
PanG, a new ketopantoate reduc ...
Francisella tularensis subsp. novicida
J. Bacteriol.
195
965-976
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736827
Tomita
Identification and characteriz ...
Thermococcus kodakarensis, Thermococcus kodakarensis KUW1
Mol. Microbiol.
90
307-321
2013
-
-
1
-
1
-
1
7
-
-
2
4
-
8
-
-
1
-
-
-
-
-
13
1
1
1
1
6
1
-
-
4
1
-
-
-
-
1
4
-
1
-
-
1
1
7
-
-
2
4
-
-
-
1
-
-
-
-
13
1
1
1
1
6
1
-
-
-
-
2
2
-
6
6
696603
Headey
Backbone assignments of the 34 ...
Escherichia coli
Biomol. NMR Assign.
2
93-96
2008
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
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-
-
-
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-
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1
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-
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-
-
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1
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684140
Ciulli
pH-tuneable binding of 2-phosp ...
Escherichia coli
Acta Crystallogr. Sect. D
63
171-178
2007
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687675
Ciulli
Crystal structure of Escherich ...
Escherichia coli
J. Biol. Chem.
282
8487-8497
2007
-
-
1
1
6
-
-
3
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
1
6
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
669806
Ciulli
Probing hot spots at protein-l ...
Escherichia coli
J. Med. Chem.
49
4992-5000
2006
-
-
-
-
-
-
7
1
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
3
6
-
-
-
-
-
3
-
-
-
-
7
6
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
667561
Ciulli
Biophysical tools to monitor e ...
Escherichia coli
Biochem. Soc. Trans.
33
767-771
2005
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
2
-
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1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667687
Lobley
The crystal structure of Esche ...
Escherichia coli
Biochemistry
44
8930-8939
2005
-
-
1
1
7
-
-
1
-
-
-
1
-
2
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
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2
-
-
-
-
-
1
2
1
7
-
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1
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1
-
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1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654677
Zheng
Substrate specificity and kine ...
Escherichia coli
Biochemistry
42
11289-11296
2003
-
-
1
-
-
-
-
8
-
-
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
1
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
656330
Merkamm
Ketopantoate reductase activit ...
Corynebacterium glutamicum
J. Biotechnol.
104
253-260
2003
-
-
1
-
-
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286110
Matak-Vinkovic
Crystal structure of Escherich ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Stenotrophomonas maltophilia, Stenotrophomonas maltophilia 845
Biochemistry
40
14493-14500
2001
-
-
1
1
1
-
-
4
-
2
4
2
-
7
-
1
1
-
-
-
2
-
4
3
-
-
-
2
-
-
-
3
-
-
-
-
-
1
3
1
1
-
-
-
-
4
-
2
4
2
-
-
1
1
-
-
2
-
4
3
-
-
-
2
-
-
-
-
-
-
-
-
-
-
286108
Zheng
Kinetic and mechanistic analys ...
Escherichia coli
Biochemistry
39
3708-3717
2000
-
-
1
-
-
-
2
4
-
1
4
1
-
2
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
4
-
1
4
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286109
Zheng
Identification of active site ...
Escherichia coli
Biochemistry
39
16244-16251
2000
5
-
1
-
10
-
-
12
-
1
1
-
-
2
-
-
1
-
-
-
1
-
2
1
-
-
-
-
1
-
-
2
-
-
-
5
-
1
2
-
10
-
-
-
-
12
-
1
1
-
-
-
-
1
-
-
1
-
2
1
-
-
-
-
1
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286106
Elischewski
Pantothenate production in Esc ...
Escherichia coli
J. Biotechnol.
75
135-146
1999
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1
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1
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286107
Frodyma
ApbA, the ketopantoate reducta ...
Escherichia coli, Escherichia coli BL21/lambdaDE3, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Stenotrophomonas maltophilia 845, Stenotrophomonas maltophilia
J. Biol. Chem.
273
5572-5576
1998
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3
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1
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1
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8
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8
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6
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12
2
1
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1
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286102
Kataoka
-
Novel enzymic production of D- ...
Agrobacterium sp., Agrobacterium tumefaciens, Pseudomonas putida, Stenotrophomonas maltophilia
Agric. Biol. Chem.
54
177-182
1990
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4
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4
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2
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286103
Shimizu
Ketopantoic acid reductase of ...
Saccharomyces cerevisiae, Stenotrophomonas maltophilia, Stenotrophomonas maltophilia 845
J. Biol. Chem.
263
12077-12084
1988
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1
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10
4
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1
4
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6
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6
1
1
1
3
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4
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1
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10
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4
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1
4
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1
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2
1
6
1
1
1
3
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2
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2
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286105
Wilken
Ketopantoic acid and ketopanto ...
Escherichia coli, Saccharomyces cerevisiae
J. Biol. Chem.
250
2311-2314
1975
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2
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286104
King
-
Separation and preliminary stu ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae NRRL Y-2034
J. Biol. Chem.
247
4096-4098
1972
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1
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