BRENDA - Enzyme Database show
show all sequences of 1.1.1.169

Ketopantoic acid reductase of Pseudomonas maltophilia 845. Purification, characterization, and role in pantothenate biosynthesis

Shimizu, S.; Kataoka, M.; Ching-Ming Chung, M.; Yamada, H.; J. Biol. Chem. 263, 12077-12084 (1988)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
fine needle, at about 40% ammonium sulfate saturation
Stenotrophomonas maltophilia
Inhibitors
Inhibitors
Commentary
Organism
Structure
Barbital
9.0% at 1 mM
Stenotrophomonas maltophilia
D-pantoic acid
noncompetitive with ketopantoic acid at saturation level of NADPH, noncompetitive with NADPH at unsaturation level of ketopantoic acid
Stenotrophomonas maltophilia
Diphenylhydantoin
22.6% at 1 mM
Stenotrophomonas maltophilia
iodoacetate
sulfhydryl reagents, slightly inhibitory
Stenotrophomonas maltophilia
NaAsO2
carbonyl reagent
Stenotrophomonas maltophilia
NADP+
competitive with NADPH at unsaturation level of ketopantoic acid
Stenotrophomonas maltophilia
p-chloromercuribenzoate
sulfhydryl reagents, slightly inhibitory
Stenotrophomonas maltophilia
phenylhydrazine
carbonyl reagent
Stenotrophomonas maltophilia
quercetin
12.0% at 0.1 mM
Stenotrophomonas maltophilia
Semicarbazide
carbonyl reagent
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0318
-
NADPH
pH 7.0
Stenotrophomonas maltophilia
0.4
-
ketopantoic acid
pH 7.0
Stenotrophomonas maltophilia
8.55
-
2-Keto-3-hydroxyisovalerate
-
Stenotrophomonas maltophilia
52.1
-
D-pantoic acid
pH 8.0
Stenotrophomonas maltophilia
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
Mg2+ independent
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30500
-
3-5 * 30500 or 30000, SDS-PAGE
Stenotrophomonas maltophilia
87000
-
+/-5000, sedimentation equilibrium method
Stenotrophomonas maltophilia
115000
-
+/-5000, gel filtration
Stenotrophomonas maltophilia
140000
160000
+/-5000, HPLC gel filtration
Stenotrophomonas maltophilia
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Stenotrophomonas maltophilia
-
845
-
Stenotrophomonas maltophilia 845
-
845
-
Purification (Commentary)
Commentary
Organism
gel chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
633
-
crystalline enzyme
Stenotrophomonas maltophilia
669
-
-
Stenotrophomonas maltophilia
Storage Stability
Storage Stability
Organism
4°C, 0.01 M Tris/HCl buffer, pH 7.4, 0.1 mM DTT, 0.2 M NaCl, 6 months
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-3-hydroxyisovalerate + NADPH
-
286103
Stenotrophomonas maltophilia
? + NADP+
-
286103
Stenotrophomonas maltophilia
r
2-keto-3-hydroxyisovalerate + NADPH
-
286103
Stenotrophomonas maltophilia 845
? + NADP+
-
286103
Stenotrophomonas maltophilia 845
r
ketopantoic acid + NADPH
-
286103
Stenotrophomonas maltophilia
D-pantoic acid + NADP+
-
286103
Stenotrophomonas maltophilia
r
ketopantoic acid + NADPH
-
286103
Stenotrophomonas maltophilia 845
D-pantoic acid + NADP+
-
286103
Stenotrophomonas maltophilia 845
r
additional information
high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate
286103
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate
286103
Stenotrophomonas maltophilia 845
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
oligomer
3-5 * 30500 or 30000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
reduction of ketopantoic acid
Stenotrophomonas maltophilia
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
60
15°C: about 70% of activity maximum, 60°C: about 85% of activity maximum
Stenotrophomonas maltophilia
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
30 min, pH 6-10
Stenotrophomonas maltophilia
60
-
10 min, stable below 60°C
Stenotrophomonas maltophilia
70
-
10 min, 70% loss of activity
Stenotrophomonas maltophilia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of ketopantoic acid
Stenotrophomonas maltophilia
8.5
-
oxidation of D-pantoic acid
Stenotrophomonas maltophilia
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
30°C, 30 min
Stenotrophomonas maltophilia
7
-
stable below 60°C for 10 min
Stenotrophomonas maltophilia
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Stenotrophomonas maltophilia
NADP+
-
Stenotrophomonas maltophilia
NADPH
specific
Stenotrophomonas maltophilia
NADPH
-
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Stenotrophomonas maltophilia
NADP+
-
Stenotrophomonas maltophilia
NADPH
specific
Stenotrophomonas maltophilia
NADPH
-
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
fine needle, at about 40% ammonium sulfate saturation
Stenotrophomonas maltophilia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Barbital
9.0% at 1 mM
Stenotrophomonas maltophilia
D-pantoic acid
noncompetitive with ketopantoic acid at saturation level of NADPH, noncompetitive with NADPH at unsaturation level of ketopantoic acid
Stenotrophomonas maltophilia
Diphenylhydantoin
22.6% at 1 mM
Stenotrophomonas maltophilia
iodoacetate
sulfhydryl reagents, slightly inhibitory
Stenotrophomonas maltophilia
NaAsO2
carbonyl reagent
Stenotrophomonas maltophilia
NADP+
competitive with NADPH at unsaturation level of ketopantoic acid
Stenotrophomonas maltophilia
p-chloromercuribenzoate
sulfhydryl reagents, slightly inhibitory
Stenotrophomonas maltophilia
phenylhydrazine
carbonyl reagent
Stenotrophomonas maltophilia
quercetin
12.0% at 0.1 mM
Stenotrophomonas maltophilia
Semicarbazide
carbonyl reagent
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0318
-
NADPH
pH 7.0
Stenotrophomonas maltophilia
0.4
-
ketopantoic acid
pH 7.0
Stenotrophomonas maltophilia
8.55
-
2-Keto-3-hydroxyisovalerate
-
Stenotrophomonas maltophilia
52.1
-
D-pantoic acid
pH 8.0
Stenotrophomonas maltophilia
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
Mg2+ independent
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30500
-
3-5 * 30500 or 30000, SDS-PAGE
Stenotrophomonas maltophilia
87000
-
+/-5000, sedimentation equilibrium method
Stenotrophomonas maltophilia
115000
-
+/-5000, gel filtration
Stenotrophomonas maltophilia
140000
160000
+/-5000, HPLC gel filtration
Stenotrophomonas maltophilia
Purification (Commentary) (protein specific)
Commentary
Organism
gel chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
633
-
crystalline enzyme
Stenotrophomonas maltophilia
669
-
-
Stenotrophomonas maltophilia
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.01 M Tris/HCl buffer, pH 7.4, 0.1 mM DTT, 0.2 M NaCl, 6 months
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-3-hydroxyisovalerate + NADPH
-
286103
Stenotrophomonas maltophilia
? + NADP+
-
286103
Stenotrophomonas maltophilia
r
2-keto-3-hydroxyisovalerate + NADPH
-
286103
Stenotrophomonas maltophilia 845
? + NADP+
-
286103
Stenotrophomonas maltophilia 845
r
ketopantoic acid + NADPH
-
286103
Stenotrophomonas maltophilia
D-pantoic acid + NADP+
-
286103
Stenotrophomonas maltophilia
r
ketopantoic acid + NADPH
-
286103
Stenotrophomonas maltophilia 845
D-pantoic acid + NADP+
-
286103
Stenotrophomonas maltophilia 845
r
additional information
high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate
286103
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate
286103
Stenotrophomonas maltophilia 845
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
oligomer
3-5 * 30500 or 30000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
reduction of ketopantoic acid
Stenotrophomonas maltophilia
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
60
15°C: about 70% of activity maximum, 60°C: about 85% of activity maximum
Stenotrophomonas maltophilia
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
30 min, pH 6-10
Stenotrophomonas maltophilia
60
-
10 min, stable below 60°C
Stenotrophomonas maltophilia
70
-
10 min, 70% loss of activity
Stenotrophomonas maltophilia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of ketopantoic acid
Stenotrophomonas maltophilia
8.5
-
oxidation of D-pantoic acid
Stenotrophomonas maltophilia
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
30°C, 30 min
Stenotrophomonas maltophilia
7
-
stable below 60°C for 10 min
Stenotrophomonas maltophilia
Other publictions for EC 1.1.1.169
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725293
Miller
PanG, a new ketopantoate reduc ...
Francisella tularensis subsp. novicida
J. Bacteriol.
195
965-976
2013
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12
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1
1
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736827
Tomita
Identification and characteriz ...
Thermococcus kodakarensis, Thermococcus kodakarensis KUW1
Mol. Microbiol.
90
307-321
2013
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-
1
-
1
-
1
7
-
-
2
4
-
8
-
-
1
-
-
-
-
-
13
1
1
1
1
6
1
-
-
4
1
-
-
-
-
1
4
-
1
-
-
1
1
7
-
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2
4
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1
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-
13
1
1
1
1
6
1
-
-
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-
2
2
-
6
6
696603
Headey
Backbone assignments of the 34 ...
Escherichia coli
Biomol. NMR Assign.
2
93-96
2008
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1
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1
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1
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684140
Ciulli
pH-tuneable binding of 2-phosp ...
Escherichia coli
Acta Crystallogr. Sect. D
63
171-178
2007
-
-
-
1
-
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1
-
2
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3
-
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1
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1
1
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1
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3
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687675
Ciulli
Crystal structure of Escherich ...
Escherichia coli
J. Biol. Chem.
282
8487-8497
2007
-
-
1
1
6
-
-
3
-
-
-
1
-
2
-
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2
-
1
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2
1
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2
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1
2
1
6
-
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3
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1
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2
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1
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2
1
-
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669806
Ciulli
Probing hot spots at protein-l ...
Escherichia coli
J. Med. Chem.
49
4992-5000
2006
-
-
-
-
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7
1
-
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1
-
2
-
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-
1
-
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-
2
-
-
-
-
-
1
-
-
3
6
-
-
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-
-
3
-
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7
6
1
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1
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2
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1
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667561
Ciulli
Biophysical tools to monitor e ...
Escherichia coli
Biochem. Soc. Trans.
33
767-771
2005
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1
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1
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1
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2
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667687
Lobley
The crystal structure of Esche ...
Escherichia coli
Biochemistry
44
8930-8939
2005
-
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1
1
7
-
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1
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1
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2
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1
1
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2
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2
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1
2
1
7
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1
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1
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2
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654677
Zheng
Substrate specificity and kine ...
Escherichia coli
Biochemistry
42
11289-11296
2003
-
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1
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-
8
-
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1
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2
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7
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1
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1
1
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4
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1
4
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8
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1
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7
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1
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1
1
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-
656330
Merkamm
Ketopantoate reductase activit ...
Corynebacterium glutamicum
J. Biotechnol.
104
253-260
2003
-
-
1
-
-
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1
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1
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3
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2
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2
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1
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1
1
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1
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2
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2
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286110
Matak-Vinkovic
Crystal structure of Escherich ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Stenotrophomonas maltophilia, Stenotrophomonas maltophilia 845
Biochemistry
40
14493-14500
2001
-
-
1
1
1
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-
4
-
2
4
2
-
7
-
1
1
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2
-
4
3
-
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2
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3
-
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1
3
1
1
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4
-
2
4
2
-
-
1
1
-
-
2
-
4
3
-
-
-
2
-
-
-
-
-
-
-
-
-
-
286108
Zheng
Kinetic and mechanistic analys ...
Escherichia coli
Biochemistry
39
3708-3717
2000
-
-
1
-
-
-
2
4
-
1
4
1
-
2
-
-
1
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1
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2
1
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-
-
-
-
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2
-
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1
2
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2
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4
-
1
4
1
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1
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1
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2
1
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-
-
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-
-
-
-
286109
Zheng
Identification of active site ...
Escherichia coli
Biochemistry
39
16244-16251
2000
5
-
1
-
10
-
-
12
-
1
1
-
-
2
-
-
1
-
-
-
1
-
2
1
-
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-
1
-
-
2
-
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5
-
1
2
-
10
-
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12
-
1
1
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1
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1
-
2
1
-
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1
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-
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286106
Elischewski
Pantothenate production in Esc ...
Escherichia coli
J. Biotechnol.
75
135-146
1999
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1
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1
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3
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1
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1
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1
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1
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286107
Frodyma
ApbA, the ketopantoate reducta ...
Escherichia coli, Escherichia coli BL21/lambdaDE3, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Stenotrophomonas maltophilia 845, Stenotrophomonas maltophilia
J. Biol. Chem.
273
5572-5576
1998
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1
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2
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1
3
-
10
-
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1
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6
-
12
2
1
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1
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8
-
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1
8
-
-
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-
-
-
2
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1
3
-
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1
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6
-
12
2
1
-
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1
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-
286102
Kataoka
-
Novel enzymic production of D- ...
Agrobacterium sp., Agrobacterium tumefaciens, Pseudomonas putida, Stenotrophomonas maltophilia
Agric. Biol. Chem.
54
177-182
1990
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4
-
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4
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2
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3
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3
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4
-
2
-
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-
-
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286103
Shimizu
Ketopantoic acid reductase of ...
Saccharomyces cerevisiae, Stenotrophomonas maltophilia, Stenotrophomonas maltophilia 845
J. Biol. Chem.
263
12077-12084
1988
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-
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1
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10
4
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1
4
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6
-
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1
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2
1
6
1
1
1
3
-
2
-
2
4
-
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4
1
-
-
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10
-
4
-
1
4
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1
-
-
2
1
6
1
1
1
3
-
2
-
2
-
-
-
-
-
-
-
286105
Wilken
Ketopantoic acid and ketopanto ...
Escherichia coli, Saccharomyces cerevisiae
J. Biol. Chem.
250
2311-2314
1975
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-
-
-
-
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2
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2
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2
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2
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2
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2
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-
2
-
-
-
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286104
King
-
Separation and preliminary stu ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae NRRL Y-2034
J. Biol. Chem.
247
4096-4098
1972
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2
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1
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2
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4
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2
1
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2
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2
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1
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2
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4
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2
1
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