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Information on EC 3.5.1.75 - urethanase
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3.5.1.75 urethanaseSpecify your search results
Word Map
- 3.5.1.75
- carbamate
-
beverage
-
wine
- nutrition
- amidase
- food industry
- ammonia
- lactobacillus
- licheniformis
-
flavor
-
cancer-causing
-
providencia
- parapsilosis
- rettgeri
-
teratogenic
-
whisky
- diamine
-
sake
-
penicillium
- liquor
- lysinibacillus
- variabile
-
decomposed
-
ultrasonication
- analysis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
urethanase, urethane hydrolase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
hydrolase, urethane
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-
-
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UreC
urethane hydrolase
urethane-hydrolyzing enzyme
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-
-
-
additional information
urethane hydrolase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
urethane + H2O = ethanol + CO2 + NH3
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
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-
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SYSTEMATIC NAME
IUBMB Comments
urethane amidohydrolase (decarboxylating)
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CAS REGISTRY NUMBER
COMMENTARY
122007-70-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hexamethylene dicarbamic acid dibutyl ester + H2O
?
-
-
product identification and quantification, overview
-
?
methylene bisphenyl dicarbamic acid dibutyl ester + H2O
?
-
-
product identification and quantification, overview
-
?
toluene-2,4-dicarbamic acid dibutyl ester + H2O
toluene diamine + ?
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-
product identification and quantification, overview
-
?
benzylcarbamate + H2O
CO2 + NH3 + benzoate
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132% of the activity with ethylcarbamate
-
-
?
benzylcarbamate + H2O
CO2 + NH3 + benzoate
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148% of the activity with ethylcarbamate
-
-
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
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urethane, potentially carcinogenic, mutagenic and teratogenic to human
equimolar amounts of ammonia and ethanol
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
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urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
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urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
equimolar amounts of ammonia and ethanol, noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
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urethane, potentially carcinogenic, mutagenic and teratogenic to human
equimolar amounts of ammonia and ethanol, noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
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enzyme may play an important role in detoxification of urethane
equimolar amounts of ammonia and ethanol, noncarcinogenic compounds
?
methyl carbamate + H2O
methanol + CO2 + NH3
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20% of the activity with ethyl carbamate, i.e. urethane
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-
?
methyl carbamate + H2O
methanol + CO2 + NH3
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20% of the activity with ethyl carbamate, i.e. urethane
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-
?
methylcarbamate + H2O
CO2 + NH3 + methanol
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64% of the activity with ethylcarbamate
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-
?
methylcarbamate + H2O
CO2 + NH3 + methanol
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64% of the activity with ethylcarbamate
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-
?
n-butylcarbamate + H2O
CO2 + NH3 + n-butanol
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192% of the activity with ethylcarbamate
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-
?
n-butylcarbamate + H2O
CO2 + NH3 + n-butanol
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224% of the activity with ethylcarbamate
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-
?
phenylcarbamate + H2O
CO2 + NH3 + phenol
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197% of the activity with ethylcarbamate
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-
?
phenylcarbamate + H2O
CO2 + NH3 + phenol
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96% of the activity with ethylcarbamate
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-
?
tert-butylcarbamate + H2O
CO2 + NH3 + tert-butanol
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118% of the activity with ethylcarbamate
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-
?
tert-butylcarbamate + H2O
CO2 + NH3 + tert-butanol
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24.4% of the activity with ethylcarbamate
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-
?
additional information
?
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hydrolyzes carbamoyl ester derivatives more rapidly than amide derivatives
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-
?
additional information
?
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no activity with 4-nitrophenyl acetate
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?
additional information
?
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no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
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?
additional information
?
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no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
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?
additional information
?
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no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide
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-
?
additional information
?
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the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
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?
additional information
?
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the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
-
-
?
additional information
?
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the enzyme exhibits not only urease activity, but also urethanase activity
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-
?
additional information
?
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high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview
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?
additional information
?
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Providencia rettgeri CGMCC 8326
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high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview
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?
additional information
?
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Providencia rettgeri CGMCC 8326
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the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
-
-
?
additional information
?
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Providencia rettgeri CGMCC 8326
-
the enzyme exhibits not only urease activity, but also urethanase activity
-
-
?
additional information
?
-
Providencia rettgeri CGMCC 8326
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the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
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-
?
additional information
?
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Providencia rettgeri JN-B815
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high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview
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-
?
additional information
?
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Providencia rettgeri JN-B815
-
the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
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-
?
additional information
?
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Providencia rettgeri JN-B815
-
the enzyme exhibits not only urease activity, but also urethanase activity
-
-
?
additional information
?
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Providencia rettgeri JN-B815
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the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
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?
additional information
?
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the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters
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?
additional information
?
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substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate
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?
additional information
?
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Rhodococcus equi TB-60
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the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters
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?
additional information
?
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Rhodococcus equi TB-60
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substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate
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?
additional information
?
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no or poor activity with gamma-aminobutyric acid, glutamic acid, and glycine
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?
additional information
?
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a highly sensitive spectrophotometric method for ethyl carbamate (EC) determination is established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 300-5000 nM, with a low detection limit of 9.28 nM. Method optimization. The absorbance declines slowly at either pH 4.5 or pH 8.0, which is around the optimal pH of one of the enzymes. But the absorbance decreases with the fastest rate at pH 6.0
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?
additional information
?
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a highly sensitive spectrophotometric method for ethyl carbamate (EC) determination is established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 300-5000 nM, with a low detection limit of 9.28 nM. Method optimization. The absorbance declines slowly at either pH 4.5 or pH 8.0, which is around the optimal pH of one of the enzymes. But the absorbance decreases with the fastest rate at pH 6.0
-
-
?
additional information
?
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no or poor activity with gamma-aminobutyric acid, glutamic acid, and glycine
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
toluene-2,4-dicarbamic acid dibutyl ester + H2O
toluene diamine + ?
-
-
-
-
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
urethane, potentially carcinogenic, mutagenic and teratogenic to human
equimolar amounts of ammonia and ethanol, noncarcinogenic compounds
?
ethylcarbamate + H2O
CO2 + NH3 + ethanol
-
enzyme may play an important role in detoxification of urethane
equimolar amounts of ammonia and ethanol, noncarcinogenic compounds
?
additional information
?
-
-
the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
-
-
?
additional information
?
-
-
the enzyme exhibits not only urease activity, but also urethanase activity
-
-
?
additional information
?
-
Providencia rettgeri CGMCC 8326
-
the enzyme exhibits not only urease activity, but also urethanase activity
-
-
?
additional information
?
-
Providencia rettgeri CGMCC 8326
-
the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
-
-
?
additional information
?
-
Providencia rettgeri JN-B815
-
the enzyme exhibits not only urease activity, but also urethanase activity
-
-
?
additional information
?
-
Providencia rettgeri JN-B815
-
the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity
-
-
?
additional information
?
-
-
the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters
-
-
?
additional information
?
-
Rhodococcus equi TB-60
-
the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe3+
-
strain IFO 12107, iron-containing urethanase, contains a typical high-spin Fe3+
additional information
additional information
-
no effect, activation by 1 mM Co2+, Ni2+, Mn2+, Cu2+, Mg2+, Zn2+, Fe2+, Ca2+
additional information
-
no effect of Ca2+, Mg2+, Na+, major cations of sea water
additional information
-
no effect, activation by Co2+, Ni2+, Mn2+, Fe2+, Cu2+, Ca2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
bathophenanthroline
-
complete inhibition, activity fully restored by addition of Fe3+ at a ratio of 4 iron atoms to 1 mol inactive protein, the apoenzyme
EDTA
-
strong inhibition, 0.00074 mM: 50% inhibition. Complete inhibition fully recovered by addition of Zn2+, but no activity recovered by addition of Mg2+
additional information
-
no inhibition by 1 mM EDTA and 2-mercaptoethanol
-
additional information
-
no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM
-
additional information
-
no or poor inhibition by Mn2+, EDTA, 8-hydroxyquinoline, DTT, and iodoacetamide
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
slight activation, activation depending on increasing concentrations of Triton X-100
additional information
-
the enzyme is strongly induced by acetanilide, effect of carbon sources and inducers on cell growth, overview
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.8
-
purified recombinant apoenzyme, urethanase activity, pH 4.5, temperature not specified in the publication
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5
6 - 8
7.5
-
strain 1013, pH-optimum at, a second, higher pH-optimum at pH 4.5
additional information
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
urethanase activity sharply declines in alkaline region