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show all sequences of 3.5.1.75

Purification and characterization of iron-containing urethanase from Bacillus licheniformis

Zhao, C.J.; Kobashi, K.; Biol. Pharm. Bull. 17, 773-778 (1994)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages; strain IFO 12107, enzyme may be a practical means of removing urethane from alcoholic beverages, because its higher activity under acidic conditions, pH 4.5, its high ethanol resistance and its low Km value for urethane
Bacillus licheniformis
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong inhibition
Bacillus licheniformis
bathophenanthroline
complete inhibition, activity fully restored by addition of Fe3+ at a ratio of 4 iron atoms to 1 mol inactive protein, the apoenzyme
Bacillus licheniformis
EDTA
no inhibition
Bacillus licheniformis
additional information
no inhibition by 1 mM EDTA or 1 mM EGTA
Bacillus licheniformis
phenylmethylsulfonyl fluoride
PMSF
Bacillus licheniformis
phosphoramidon
-
Bacillus licheniformis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Bacillus licheniformis
0.17
-
urethane
strain IFO 12107
Bacillus licheniformis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe3+
strain IFO 12107, iron-containing urethanase, contains a typical high-spin Fe3+
Bacillus licheniformis
additional information
no effect, activation by 1 mM Co2+, Ni2+, Mn2+, Cu2+, Mg2+, Zn2+, Fe2+, Ca2+
Bacillus licheniformis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
strain IFO 12107, homotetramer, 4 * 42000, SDS-PAGE
Bacillus licheniformis
160000
-
strain IFO 12107, gel filtration
Bacillus licheniformis
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Ethanol
strain IFO 12107, considerably resistant to high concentrations of ethanol, 64% of the activity retained in 20% ethanol, great advantage for industrial removal of urethane from alcoholic beverages
Bacillus licheniformis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus licheniformis
-
eight strains, highest activity in strain IFO 12107; gastrointestinal bacterium
-
Purification (Commentary)
Commentary
Organism
strain IFO 12107
Bacillus licheniformis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus licheniformis
0.041
-
strain IFO 12107
Bacillus licheniformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetamide + H2O
78% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
benzamide + H2O
30% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
benzylcarbamate + H2O
132% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + benzoate
-
-
-
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209235
Bacillus licheniformis
CO2 + NH3 + ethanol
noncarcinogenic compounds
209235
Bacillus licheniformis
?
methylcarbamate + H2O
64% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + methanol
-
-
-
?
additional information
substrate specificity
209235
Bacillus licheniformis
?
-
-
-
-
additional information
hydrolyzes carbamoyl ester derivatives more rapidly than amide derivatives
209235
Bacillus licheniformis
?
-
-
-
-
additional information
no activity with 4-nitrophenyl acetate
209235
Bacillus licheniformis
?
-
-
-
-
additional information
no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
209235
Bacillus licheniformis
?
-
-
-
-
n-butylcarbamate + H2O
192% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + n-butanol
-
-
-
?
n-butyramide + H2O
93% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
phenylcarbamate + H2O
197% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + phenol
-
-
-
?
tert-butylcarbamate + H2O
118% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + tert-butanol
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
strain IFO 12107, homotetramer, 4 * 42000, SDS-PAGE
Bacillus licheniformis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Bacillus licheniformis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
strain IFO 12107, stable below
Bacillus licheniformis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.5; significantly higher urethanase activity at acidic condition than at neutral condition
Bacillus licheniformis
4.5
-
-
Bacillus licheniformis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
strain IFO 12107, stable between
Bacillus licheniformis
Application (protein specific)
Application
Commentary
Organism
nutrition
great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages; strain IFO 12107, enzyme may be a practical means of removing urethane from alcoholic beverages, because its higher activity under acidic conditions, pH 4.5, its high ethanol resistance and its low Km value for urethane
Bacillus licheniformis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong inhibition
Bacillus licheniformis
bathophenanthroline
complete inhibition, activity fully restored by addition of Fe3+ at a ratio of 4 iron atoms to 1 mol inactive protein, the apoenzyme
Bacillus licheniformis
EDTA
no inhibition
Bacillus licheniformis
additional information
no inhibition by 1 mM EDTA or 1 mM EGTA
Bacillus licheniformis
phenylmethylsulfonyl fluoride
PMSF
Bacillus licheniformis
phosphoramidon
-
Bacillus licheniformis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Bacillus licheniformis
0.17
-
urethane
strain IFO 12107
Bacillus licheniformis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe3+
strain IFO 12107, iron-containing urethanase, contains a typical high-spin Fe3+
Bacillus licheniformis
additional information
no effect, activation by 1 mM Co2+, Ni2+, Mn2+, Cu2+, Mg2+, Zn2+, Fe2+, Ca2+
Bacillus licheniformis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
strain IFO 12107, homotetramer, 4 * 42000, SDS-PAGE
Bacillus licheniformis
160000
-
strain IFO 12107, gel filtration
Bacillus licheniformis
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Ethanol
strain IFO 12107, considerably resistant to high concentrations of ethanol, 64% of the activity retained in 20% ethanol, great advantage for industrial removal of urethane from alcoholic beverages
Bacillus licheniformis
Purification (Commentary) (protein specific)
Commentary
Organism
strain IFO 12107
Bacillus licheniformis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus licheniformis
0.041
-
strain IFO 12107
Bacillus licheniformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetamide + H2O
78% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
benzamide + H2O
30% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
benzylcarbamate + H2O
132% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + benzoate
-
-
-
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209235
Bacillus licheniformis
CO2 + NH3 + ethanol
noncarcinogenic compounds
209235
Bacillus licheniformis
?
methylcarbamate + H2O
64% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + methanol
-
-
-
?
additional information
substrate specificity
209235
Bacillus licheniformis
?
-
-
-
-
additional information
hydrolyzes carbamoyl ester derivatives more rapidly than amide derivatives
209235
Bacillus licheniformis
?
-
-
-
-
additional information
no activity with 4-nitrophenyl acetate
209235
Bacillus licheniformis
?
-
-
-
-
additional information
no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
209235
Bacillus licheniformis
?
-
-
-
-
n-butylcarbamate + H2O
192% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + n-butanol
-
-
-
?
n-butyramide + H2O
93% of the activity with ethylcarbamate
209235
Bacillus licheniformis
?
-
-
-
?
phenylcarbamate + H2O
197% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + phenol
-
-
-
?
tert-butylcarbamate + H2O
118% of the activity with ethylcarbamate
209235
Bacillus licheniformis
CO2 + NH3 + tert-butanol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
strain IFO 12107, homotetramer, 4 * 42000, SDS-PAGE
Bacillus licheniformis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Bacillus licheniformis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
strain IFO 12107, stable below
Bacillus licheniformis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.5; significantly higher urethanase activity at acidic condition than at neutral condition
Bacillus licheniformis
4.5
-
-
Bacillus licheniformis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
strain IFO 12107, stable between
Bacillus licheniformis
Other publictions for EC 3.5.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733136
Zhou
Purification and characterizat ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
172
351-360
2014
-
1
-
-
-
-
-
1
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
733131
Zhou
Isolation and characterization ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
170
718-728
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
7
1
-
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
1
1
-
-
-
733132
Wu
Immobilized Rhodotorula mucila ...
Rhodotorula mucilaginosa, Rhodotorula mucilaginosa CGMCC 5081
Appl. Biochem. Biotechnol.
171
2220-2232
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667283
Akutsu-Shigeno
Isolation of a bacterium that ...
Rhodococcus hoagii, Rhodococcus hoagii TB-60
Appl. Microbiol. Biotechnol.
70
422-429
2006
1
-
1
-
-
-
9
11
-
2
1
5
-
4
-
-
1
-
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
1
-
1
-
-
-
-
-
9
-
11
-
2
1
5
-
-
-
1
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
209239
Mohapatra
-
Characterization of urethanase ...
Micrococcus sp.
Lett. Appl. Microbiol.
25
393-396
1997
-
1
-
-
-
-
12
2
-
1
-
-
1
1
-
-
1
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
1
-
-
-
-
-
-
12
-
2
-
1
-
-
1
-
-
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
-
-
209235
Zhao
Purification and characterizat ...
Bacillus licheniformis
Biol. Pharm. Bull.
17
773-778
1994
-
1
-
-
-
-
6
2
-
2
2
-
1
3
-
-
1
-
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
2
-
2
2
-
1
-
-
1
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
209238
Zhao
Urethanase in rat tissues ...
Rattus norvegicus
J. Toxicol. Environ. Health
37
37-45
1992
1
1
-
-
-
-
1
-
5
2
-
4
-
2
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
5
2
-
4
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
-
-
-
209236
Zhao
Urethanase of Bacillus licheni ...
Bacillus licheniformis, Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus IAM 1034, Purpureocillium lilacinum
Chem. Pharm. Bull.
39
3303-3306
1991
-
1
-
-
-
-
2
4
1
1
-
-
3
7
-
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
4
1
1
-
-
3
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
-
-
209237
Kobashi
Urethane-hydrolyzing enzyme fr ...
Citrobacter sp.
Chem. Pharm. Bull.
38
1326-1328
1990
-
1
-
-
-
-
12
1
1
1
-
-
1
4
-
-
1
-
-
2
2
-
13
-
1
-
1
-
1
1
-
1
-
-
-
-
1
-
1
-
-
-
-
12
-
1
1
1
-
-
1
-
-
1
-
2
2
-
13
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-