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Literature summary for 3.5.1.75 extracted from
- Urethanase of Bacillus licheniformis sp. isolated from mouse gastrointestine (1991), Chem. Pharm. Bull., 39, 3303-3306.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no inhibition | Bacillus licheniformis |  |
| additional information | no inhibition by 1 mM EDTA and 2-mercaptoethanol | Bacillus licheniformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
urethane | - |
Paenibacillus thiaminolyticus | |
| 42 | - |
urethane | strain 1013, at pH 4.5 | Bacillus licheniformis | |
| 50 | - |
urethane | - |
Purpureocillium lilacinum | |
| 56 | - |
urethane | strain 1013, at pH 7.5 | Bacillus licheniformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | strain 1013, 70% of activity in a cytosolic fraction | Bacillus licheniformis | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions not required | Bacillus licheniformis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | no resistance to ethanol | Purpureocillium lilacinum |
| Ethanol | strain 1013, 20% ethanol, 54% residual activity after 2 h at 37°C and pH 4.5 | Bacillus licheniformis |
| Ethanol | 20% ethanol, 20% residual activity after 2 h at 37?C | Paenibacillus thiaminolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
sp. 1013, isolated from mouse gastrointestine | - |
| Bacillus licheniformis | - |
gastrointestinal bacterium | - |
| Paenibacillus thiaminolyticus | - |
IAM 1034 | - |
| Paenibacillus thiaminolyticus IAM 1034 | - |
IAM 1034 | - |
| Purpureocillium lilacinum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | urethanase activity detected in the cells not in the culture medium | Bacillus licheniformis | - |
| additional information | urethanase activity detected in the cells not in the culture medium | Bacillus licheniformis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Bacillus licheniformis | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Bacillus licheniformis | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Purpureocillium lilacinum | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Paenibacillus thiaminolyticus | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Paenibacillus thiaminolyticus IAM 1034 | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | strain 1013, about 50% of maximum activity at 20°C and 50°C | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
strain 1013, pH 4.5-7.5, 30 min, stable at | Bacillus licheniformis |
| 50 | - |
strain 1013, 1 h, complete loss of activity | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
significantly higher urethanase activity at acidic condition than at neutral condition | Bacillus licheniformis |
| 4.5 | - |
strain 1013, highest pH-optimum at, second pH-optimum at pH 7.5 | Bacillus licheniformis |
| 6 | 8 | - |
Paenibacillus thiaminolyticus |
| 7 | 8 | - |
Purpureocillium lilacinum |
| 7.5 | - |
strain 1013, pH-optimum at, a second, higher pH-optimum at pH 4.5 | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | strain 1013, 37°C, 30 min, stable between | Bacillus licheniformis |
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Release 2023.1 (January 2023)
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