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Literature summary for 3.5.1.75 extracted from
- Preparation of crosslinked enzyme aggregates (CLEAs) of acid urease with urethanase activity and their application (2016), J. Basic Microbiol., 56, 422-431 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | crosslinked enzyme aggregates of Providencia rettgeri urease (PRU-CLEAs) have great potential in the elimination of urethane (ethyl carbamate) from Chinese rice wine. Process flow diagram of PRU-CLEAs applied in membrane reactor, overview | Providencia rettgeri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | crosslinked enzyme aggregates of Providencia rettgeri urease (PRU-CLEAs) are prepared using genipin as crosslinking agent, method, overview. Optimal at 0.3 g/l of bovine serum albumin. The aggregates remove urea, but the treatment with PRU-CLEAs reveals no significant change of volatile flavor substances in Chinese rice wine. By using urea as the substrate, the values of Km and Vmax of free urease from Providencia rettgeri JN-B815 are estimated to be 5.99 mmol/l and 840 nmol/min, while those of immobilized urease are 13.54 mmol/l and 940 nmol/min, respectively. By using urethane as the substrate, the Km and Vmax value of free urethanase are determined to be 183.82 mmol/l and 970 nmol/min, while those of immobilized enzyme are 705.78 mmol/l and 650 nmol/min, respectively | Providencia rettgeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Providencia rettgeri | the enzyme exhibits not only urease activity, but also urethanase activity | ? | - |
? |  |
| additional information | Providencia rettgeri JN-B815 | the enzyme exhibits not only urease activity, but also urethanase activity | ? | - |
? | |
| additional information | Providencia rettgeri CGMCC 8326 | the enzyme exhibits not only urease activity, but also urethanase activity | ? | - |
? | |
| urethane + H2O | Providencia rettgeri | - |
ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | Providencia rettgeri JN-B815 | - |
ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | Providencia rettgeri CGMCC 8326 | - |
ethanol + CO2 + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Providencia rettgeri | - |
- |
- |
| Providencia rettgeri CGMCC 8326 | - |
- |
- |
| Providencia rettgeri JN-B815 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native urethanase 3.1fold by ethanol precipitation, and anion exchange chromatography | Providencia rettgeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
purified enzyme, urethanase activity, pH 4.5, 37°C | Providencia rettgeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri | ? | - |
? | |
| additional information | the enzyme exhibits not only urease activity, but also urethanase activity | Providencia rettgeri | ? | - |
? | |
| additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri JN-B815 | ? | - |
? | |
| additional information | the enzyme exhibits not only urease activity, but also urethanase activity | Providencia rettgeri JN-B815 | ? | - |
? | |
| additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri CGMCC 8326 | ? | - |
? | |
| additional information | the enzyme exhibits not only urease activity, but also urethanase activity | Providencia rettgeri CGMCC 8326 | ? | - |
? | |
| urethane + H2O | - |
Providencia rettgeri | ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | - |
Providencia rettgeri JN-B815 | ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | - |
Providencia rettgeri CGMCC 8326 | ethanol + CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61570, SDS-PAGE | Providencia rettgeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | also see for EC 3.5.1.5 | Providencia rettgeri |
| UreC | - |
Providencia rettgeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Providencia rettgeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
assay at | Providencia rettgeri |
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Release 2023.1 (January 2023)
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