BRENDA - Enzyme Database show
show all sequences of 3.5.1.75

Urethane-hydrolyzing enzyme from Citrobacter sp

Kobashi, K.; Takebe, S.; Sakai, T.; Chem. Pharm. Bull. 38, 1326-1328 (1990)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
practically ineffective for the elimination of urethane from alcoholic beverages, because the enzyme is inactive in high concentrations of alcohol, ethanol, and at acidic pH
Citrobacter sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM: complete inhibition
Citrobacter sp.
EDTA
no inhibition
Citrobacter sp.
F-
1 mM: complete inhibition
Citrobacter sp.
Hg2+
1 mM: complete inhibition
Citrobacter sp.
iodoacetamide
no inhibition at 0.1 mM
Citrobacter sp.
additional information
no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM
Citrobacter sp.
N-Benzoylphosphoric triamide
-
Citrobacter sp.
O,O-Dimethyl-O-(2,2-dichlorovinyl) phosphate
-
Citrobacter sp.
O,O-Dimethyl-O-(3-methyl-4-nitrophenyl) phosphate
-
Citrobacter sp.
phenylphosphorodiamide
-
Citrobacter sp.
phenylthiophosphorodiamide
-
Citrobacter sp.
Sodium laurylsulfate
35 mM: complete inhibition
Citrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
urethane
-
Citrobacter sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
80% of activity in a cytosolic fraction
Citrobacter sp.
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
metal ions not required
Citrobacter sp.
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Ethanol
inactive at high concentrations of alcohol, 20% ethanol, 8.9% residual activity after 2 h at 37°C
Citrobacter sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrobacter sp.
-
isolated from mouse feces
-
Purification (Commentary)
Commentary
Organism
partial
Citrobacter sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
urethanase activity detected in the cells not in the culture medium
Citrobacter sp.
-
additional information
urethanase activity detected in the cells not in the culture medium
Citrobacter sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Citrobacter sp.
0.254
-
-
Citrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetamide + H2O
880% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
benzamide + H2O
680% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
benzylcarbamate + H2O
148% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + benzoate
-
-
-
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209237
Citrobacter sp.
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209237
Citrobacter sp.
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209237
Citrobacter sp.
CO2 + NH3 + ethanol
noncarcinogenic compounds
209237
Citrobacter sp.
?
methylcarbamate + H2O
64% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + methanol
-
-
-
?
additional information
substrate specificity
209237
Citrobacter sp.
?
-
-
-
-
additional information
no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
209237
Citrobacter sp.
?
-
-
-
-
additional information
no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide
209237
Citrobacter sp.
?
-
-
-
-
n-butylcarbamate + H2O
224% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + n-butanol
-
-
-
?
n-butyramide + H2O
1000% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
phenylcarbamate + H2O
96% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + phenol
-
-
-
?
tert-butylcarbamate + H2O
24.4% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + tert-butanol
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Citrobacter sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
15 min, complete loss of activity
Citrobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
-
Citrobacter sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
-
half of maximum activity at pH 5.0
Citrobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
does not require any cofactor
Citrobacter sp.
Application (protein specific)
Application
Commentary
Organism
nutrition
practically ineffective for the elimination of urethane from alcoholic beverages, because the enzyme is inactive in high concentrations of alcohol, ethanol, and at acidic pH
Citrobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
does not require any cofactor
Citrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM: complete inhibition
Citrobacter sp.
EDTA
no inhibition
Citrobacter sp.
F-
1 mM: complete inhibition
Citrobacter sp.
Hg2+
1 mM: complete inhibition
Citrobacter sp.
iodoacetamide
no inhibition at 0.1 mM
Citrobacter sp.
additional information
no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM
Citrobacter sp.
N-Benzoylphosphoric triamide
-
Citrobacter sp.
O,O-Dimethyl-O-(2,2-dichlorovinyl) phosphate
-
Citrobacter sp.
O,O-Dimethyl-O-(3-methyl-4-nitrophenyl) phosphate
-
Citrobacter sp.
phenylphosphorodiamide
-
Citrobacter sp.
phenylthiophosphorodiamide
-
Citrobacter sp.
Sodium laurylsulfate
35 mM: complete inhibition
Citrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
urethane
-
Citrobacter sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
80% of activity in a cytosolic fraction
Citrobacter sp.
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
metal ions not required
Citrobacter sp.
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Ethanol
inactive at high concentrations of alcohol, 20% ethanol, 8.9% residual activity after 2 h at 37°C
Citrobacter sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Citrobacter sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
urethanase activity detected in the cells not in the culture medium
Citrobacter sp.
-
additional information
urethanase activity detected in the cells not in the culture medium
Citrobacter sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Citrobacter sp.
0.254
-
-
Citrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetamide + H2O
880% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
benzamide + H2O
680% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
benzylcarbamate + H2O
148% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + benzoate
-
-
-
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209237
Citrobacter sp.
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209237
Citrobacter sp.
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209237
Citrobacter sp.
CO2 + NH3 + ethanol
noncarcinogenic compounds
209237
Citrobacter sp.
?
methylcarbamate + H2O
64% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + methanol
-
-
-
?
additional information
substrate specificity
209237
Citrobacter sp.
?
-
-
-
-
additional information
no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea
209237
Citrobacter sp.
?
-
-
-
-
additional information
no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide
209237
Citrobacter sp.
?
-
-
-
-
n-butylcarbamate + H2O
224% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + n-butanol
-
-
-
?
n-butyramide + H2O
1000% of the activity with ethylcarbamate
209237
Citrobacter sp.
?
-
-
-
?
phenylcarbamate + H2O
96% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + phenol
-
-
-
?
tert-butylcarbamate + H2O
24.4% of the activity with ethylcarbamate
209237
Citrobacter sp.
CO2 + NH3 + tert-butanol
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Citrobacter sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
15 min, complete loss of activity
Citrobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
-
Citrobacter sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
-
half of maximum activity at pH 5.0
Citrobacter sp.
Other publictions for EC 3.5.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733136
Zhou
Purification and characterizat ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
172
351-360
2014
-
1
-
-
-
-
-
1
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
733131
Zhou
Isolation and characterization ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
170
718-728
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
7
1
-
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
1
1
-
-
-
733132
Wu
Immobilized Rhodotorula mucila ...
Rhodotorula mucilaginosa, Rhodotorula mucilaginosa CGMCC 5081
Appl. Biochem. Biotechnol.
171
2220-2232
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667283
Akutsu-Shigeno
Isolation of a bacterium that ...
Rhodococcus hoagii, Rhodococcus hoagii TB-60
Appl. Microbiol. Biotechnol.
70
422-429
2006
1
-
1
-
-
-
9
11
-
2
1
5
-
4
-
-
1
-
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
1
-
1
-
-
-
-
-
9
-
11
-
2
1
5
-
-
-
1
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
209239
Mohapatra
-
Characterization of urethanase ...
Micrococcus sp.
Lett. Appl. Microbiol.
25
393-396
1997
-
1
-
-
-
-
12
2
-
1
-
-
1
1
-
-
1
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
1
-
-
-
-
-
-
12
-
2
-
1
-
-
1
-
-
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
-
-
209235
Zhao
Purification and characterizat ...
Bacillus licheniformis
Biol. Pharm. Bull.
17
773-778
1994
-
1
-
-
-
-
6
2
-
2
2
-
1
3
-
-
1
-
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
2
-
2
2
-
1
-
-
1
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
209238
Zhao
Urethanase in rat tissues ...
Rattus norvegicus
J. Toxicol. Environ. Health
37
37-45
1992
1
1
-
-
-
-
1
-
5
2
-
4
-
2
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
5
2
-
4
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
-
-
-
209236
Zhao
Urethanase of Bacillus licheni ...
Bacillus licheniformis, Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus IAM 1034, Purpureocillium lilacinum
Chem. Pharm. Bull.
39
3303-3306
1991
-
1
-
-
-
-
2
4
1
1
-
-
3
7
-
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
4
1
1
-
-
3
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
-
-
209237
Kobashi
Urethane-hydrolyzing enzyme fr ...
Citrobacter sp.
Chem. Pharm. Bull.
38
1326-1328
1990
-
1
-
-
-
-
12
1
1
1
-
-
1
4
-
-
1
-
-
2
2
-
13
-
1
-
1
-
1
1
-
1
-
-
-
-
1
-
1
-
-
-
-
12
-
1
1
1
-
-
1
-
-
1
-
2
2
-
13
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-