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Literature summary for 3.5.1.75 extracted from

  • Kobashi, K.; Takebe, S.; Sakai, T.
    Urethane-hydrolyzing enzyme from Citrobacter sp (1990), Chem. Pharm. Bull., 38, 1326-1328.
    View publication on PubMed
Search for more literature for 3.5.1.75

Application

Application Comment Organism
nutrition practically ineffective for the elimination of urethane from alcoholic beverages, because the enzyme is inactive in high concentrations of alcohol, ethanol, and at acidic pH Citrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM: complete inhibition Citrobacter sp.
EDTA no inhibition Citrobacter sp.
F- 1 mM: complete inhibition Citrobacter sp.
Hg2+ 1 mM: complete inhibition Citrobacter sp.
iodoacetamide no inhibition at 0.1 mM Citrobacter sp.
additional information no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM Citrobacter sp.
N-Benzoylphosphoric triamide
-
 Citrobacter sp.
O,O-Dimethyl-O-(2,2-dichlorovinyl) phosphate
-
 Citrobacter sp.
O,O-Dimethyl-O-(3-methyl-4-nitrophenyl) phosphate
-
 Citrobacter sp.
phenylphosphorodiamide
-
 Citrobacter sp.
phenylthiophosphorodiamide
-
 Citrobacter sp.
Sodium laurylsulfate 35 mM: complete inhibition Citrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 urethane
-
 Citrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 80% of activity in a cytosolic fraction Citrobacter sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions not required Citrobacter sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol inactive at high concentrations of alcohol, 20% ethanol, 8.9% residual activity after 2 h at 37°C Citrobacter sp.

Organism

Organism UniProt Comment Textmining
Citrobacter sp.
-
 isolated from mouse feces
-

Purification (Commentary)

Purification (Comment) Organism
partial Citrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture urethanase activity detected in the cells not in the culture medium Citrobacter sp.
-
additional information urethanase activity detected in the cells not in the culture medium Citrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Citrobacter sp.
0.254
-
-
 Citrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O 880% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
benzamide + H2O 680% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
benzylcarbamate + H2O 148% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + benzoate
-
 ?
ethylcarbamate + H2O urethane, potentially carcinogenic, mutagenic and teratogenic to human Citrobacter sp. CO2 + NH3 + ethanol equimolar amounts of ammonia and ethanol ?
ethylcarbamate + H2O urethane, potentially carcinogenic, mutagenic and teratogenic to human Citrobacter sp. CO2 + NH3 + ethanol noncarcinogenic compounds ?
methylcarbamate + H2O 64% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + methanol
-
 ?
additional information substrate specificity Citrobacter sp. ?
-
 ?
additional information no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea Citrobacter sp. ?
-
 ?
additional information no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide Citrobacter sp. ?
-
 ?
n-butylcarbamate + H2O 224% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + n-butanol
-
 ?
n-butyramide + H2O 1000% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
phenylcarbamate + H2O 96% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + phenol
-
 ?
tert-butylcarbamate + H2O 24.4% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + tert-butanol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Citrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 15 min, complete loss of activity Citrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Citrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 half of maximum activity at pH 5.0 Citrobacter sp.

Cofactor

Cofactor Comment Organism Structure
additional information does not require any cofactor Citrobacter sp.