BRENDA - Enzyme Database show
show all sequences of 3.5.1.75

Urethanase in rat tissues

Zhao, C.J.; Kobashi, K.; J. Toxicol. Environ. Health 37, 37-45 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
slight activation, activation depending on increasing concentrations of Triton X-100
Rattus norvegicus
Application
Application
Commentary
Organism
nutrition
great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
strong inhibition, 0.00074 mM: 50% inhibition. Complete inhibition fully recovered by addition of Zn2+, but no activity recovered by addition of Mg2+
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
moderate activity
Rattus norvegicus
5829
-
lysosome
highest activity, located mainly in the lysosomal fraction
Rattus norvegicus
5764
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
moderate activity
Rattus norvegicus
5739
-
additional information
intracellular distribution
Rattus norvegicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect, activation by Co2+, Ni2+, Mn2+, Fe2+, Cu2+, Ca2+
Rattus norvegicus
Zn2+
probably belongs to category of Zn enzyme
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethylcarbamate + H2O
Rattus norvegicus
urethane, potentially carcinogenic, mutagenic and teratogenic to human
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
urethane, potentially carcinogenic, mutagenic and teratogenic to human
CO2 + NH3 + ethanol
noncarcinogenic compounds
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
enzyme may play an important role in detoxification of urethane
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
enzyme may play an important role in detoxification of urethane
CO2 + NH3 + ethanol
noncarcinogenic compounds
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
highest activity
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
tissue distribution
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209238
Rattus norvegicus
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209238
Rattus norvegicus
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209238
Rattus norvegicus
CO2 + NH3 + ethanol
noncarcinogenic compounds
209238
Rattus norvegicus
?
ethylcarbamate + H2O
enzyme may play an important role in detoxification of urethane
209238
Rattus norvegicus
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209238
Rattus norvegicus
?
ethylcarbamate + H2O
enzyme may play an important role in detoxification of urethane
209238
Rattus norvegicus
CO2 + NH3 + ethanol
noncarcinogenic compounds
209238
Rattus norvegicus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Rattus norvegicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable at for 1 h
Rattus norvegicus
80
-
1 h, complete loss of activity
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
Rattus norvegicus
5
-
-
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
stable between
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
slight activation, activation depending on increasing concentrations of Triton X-100
Rattus norvegicus
Application (protein specific)
Application
Commentary
Organism
nutrition
great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
strong inhibition, 0.00074 mM: 50% inhibition. Complete inhibition fully recovered by addition of Zn2+, but no activity recovered by addition of Mg2+
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
moderate activity
Rattus norvegicus
5829
-
lysosome
highest activity, located mainly in the lysosomal fraction
Rattus norvegicus
5764
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
moderate activity
Rattus norvegicus
5739
-
additional information
intracellular distribution
Rattus norvegicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect, activation by Co2+, Ni2+, Mn2+, Fe2+, Cu2+, Ca2+
Rattus norvegicus
Zn2+
probably belongs to category of Zn enzyme
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ethylcarbamate + H2O
Rattus norvegicus
urethane, potentially carcinogenic, mutagenic and teratogenic to human
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
urethane, potentially carcinogenic, mutagenic and teratogenic to human
CO2 + NH3 + ethanol
noncarcinogenic compounds
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
enzyme may play an important role in detoxification of urethane
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
Rattus norvegicus
?
ethylcarbamate + H2O
Rattus norvegicus
enzyme may play an important role in detoxification of urethane
CO2 + NH3 + ethanol
noncarcinogenic compounds
Rattus norvegicus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
highest activity
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
tissue distribution
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209238
Rattus norvegicus
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209238
Rattus norvegicus
?
ethylcarbamate + H2O
urethane, potentially carcinogenic, mutagenic and teratogenic to human
209238
Rattus norvegicus
CO2 + NH3 + ethanol
noncarcinogenic compounds
209238
Rattus norvegicus
?
ethylcarbamate + H2O
enzyme may play an important role in detoxification of urethane
209238
Rattus norvegicus
CO2 + NH3 + ethanol
equimolar amounts of ammonia and ethanol
209238
Rattus norvegicus
?
ethylcarbamate + H2O
enzyme may play an important role in detoxification of urethane
209238
Rattus norvegicus
CO2 + NH3 + ethanol
noncarcinogenic compounds
209238
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Rattus norvegicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable at for 1 h
Rattus norvegicus
80
-
1 h, complete loss of activity
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
significantly higher urethanase activity at acidic condition than at neutral condition
Rattus norvegicus
5
-
-
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
stable between
Rattus norvegicus
Other publictions for EC 3.5.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733136
Zhou
Purification and characterizat ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
172
351-360
2014
-
1
-
-
-
-
-
1
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
733131
Zhou
Isolation and characterization ...
Talaromyces variabilis, Talaromyces variabilis JN-A525
Appl. Biochem. Biotechnol.
170
718-728
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
7
1
-
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
6
-
1
-
1
-
1
1
3
-
-
1
1
-
-
-
733132
Wu
Immobilized Rhodotorula mucila ...
Rhodotorula mucilaginosa, Rhodotorula mucilaginosa CGMCC 5081
Appl. Biochem. Biotechnol.
171
2220-2232
2013
-
1
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667283
Akutsu-Shigeno
Isolation of a bacterium that ...
Rhodococcus hoagii, Rhodococcus hoagii TB-60
Appl. Microbiol. Biotechnol.
70
422-429
2006
1
-
1
-
-
-
9
11
-
2
1
5
-
4
-
-
1
-
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
1
-
1
-
-
-
-
-
9
-
11
-
2
1
5
-
-
-
1
-
1
2
-
25
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
209239
Mohapatra
-
Characterization of urethanase ...
Micrococcus sp.
Lett. Appl. Microbiol.
25
393-396
1997
-
1
-
-
-
-
12
2
-
1
-
-
1
1
-
-
1
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
1
-
-
-
-
-
-
12
-
2
-
1
-
-
1
-
-
1
-
2
-
-
1
-
1
3
3
-
2
3
-
-
-
-
-
-
-
-
209235
Zhao
Purification and characterizat ...
Bacillus licheniformis
Biol. Pharm. Bull.
17
773-778
1994
-
1
-
-
-
-
6
2
-
2
2
-
1
3
-
-
1
-
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
2
-
2
2
-
1
-
-
1
-
-
2
-
13
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
209238
Zhao
Urethanase in rat tissues ...
Rattus norvegicus
J. Toxicol. Environ. Health
37
37-45
1992
1
1
-
-
-
-
1
-
5
2
-
4
-
2
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
5
2
-
4
-
-
-
-
-
4
1
-
4
-
1
-
2
-
2
-
1
-
-
-
-
-
-
-
209236
Zhao
Urethanase of Bacillus licheni ...
Bacillus licheniformis, Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus IAM 1034, Purpureocillium lilacinum
Chem. Pharm. Bull.
39
3303-3306
1991
-
1
-
-
-
-
2
4
1
1
-
-
3
7
-
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
4
1
1
-
-
3
-
-
-
-
2
-
-
5
-
1
1
2
-
5
-
1
-
-
-
-
-
-
-
209237
Kobashi
Urethane-hydrolyzing enzyme fr ...
Citrobacter sp.
Chem. Pharm. Bull.
38
1326-1328
1990
-
1
-
-
-
-
12
1
1
1
-
-
1
4
-
-
1
-
-
2
2
-
13
-
1
-
1
-
1
1
-
1
-
-
-
-
1
-
1
-
-
-
-
12
-
1
1
1
-
-
1
-
-
1
-
2
2
-
13
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-