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Literature summary for 3.5.1.75 extracted from

  • Zhang, Z.; Lu, X.; Tian, Y.; Zhou, N.
    High-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode (2017), Electroanalysis, 29, 481-488 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information development of an amperometric biosensor for ethyl carbamate (urethane) with urethanase and glutamate dehydrogenase (GLDH). Urethanase decomposes ethyl carbamate to produce ammonia, which is converted to L-glutamate under the catalysis of GLDH in the presence of 2-oxoglutarate and NADH. The two enzymes are entrapped into chitosan/gelatine/gamma-glycidoxy propyl trimethoxy silane sol-gel and immobilized on the surface of pyrolytic graphite electrode (PGE). The modified electrode is characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the amperometric EC biosensor exhibits a linear detection range from 0.0005 to 0.040 mM with a low detection limit of 5.30 nM. The biosensor is successfully used to detect urethane in mimic Chinese rice wine samples, and satisfactory recovery and relative standard deviation are achieved Providencia rettgeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urethane + H2O Providencia rettgeri
-
ethanol + CO2 + NH3
-
?
urethane + H2O Providencia rettgeri JN-B815
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ethanol + CO2 + NH3
-
?
urethane + H2O Providencia rettgeri CGMCC 8326
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ethanol + CO2 + NH3
-
?

Organism

Organism UniProt Comment Textmining
Providencia rettgeri
-
-
-
Providencia rettgeri CGMCC 8326
-
-
-
Providencia rettgeri JN-B815
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview Providencia rettgeri ?
-
?
additional information high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview Providencia rettgeri JN-B815 ?
-
?
additional information high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview Providencia rettgeri CGMCC 8326 ?
-
?
urethane + H2O
-
Providencia rettgeri ethanol + CO2 + NH3
-
?
urethane + H2O
-
Providencia rettgeri JN-B815 ethanol + CO2 + NH3
-
?
urethane + H2O
-
Providencia rettgeri CGMCC 8326 ethanol + CO2 + NH3
-
?

Synonyms

Synonyms Comment Organism
UreC
-
Providencia rettgeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Providencia rettgeri