Protein Variants | Comment | Organism |
---|---|---|
additional information | development of an amperometric biosensor for ethyl carbamate (urethane) with urethanase and glutamate dehydrogenase (GLDH). Urethanase decomposes ethyl carbamate to produce ammonia, which is converted to L-glutamate under the catalysis of GLDH in the presence of 2-oxoglutarate and NADH. The two enzymes are entrapped into chitosan/gelatine/gamma-glycidoxy propyl trimethoxy silane sol-gel and immobilized on the surface of pyrolytic graphite electrode (PGE). The modified electrode is characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the amperometric EC biosensor exhibits a linear detection range from 0.0005 to 0.040 mM with a low detection limit of 5.30 nM. The biosensor is successfully used to detect urethane in mimic Chinese rice wine samples, and satisfactory recovery and relative standard deviation are achieved | Providencia rettgeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urethane + H2O | Providencia rettgeri | - |
ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | Providencia rettgeri JN-B815 | - |
ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | Providencia rettgeri CGMCC 8326 | - |
ethanol + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia rettgeri | - |
- |
- |
Providencia rettgeri CGMCC 8326 | - |
- |
- |
Providencia rettgeri JN-B815 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview | Providencia rettgeri | ? | - |
? | |
additional information | high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview | Providencia rettgeri JN-B815 | ? | - |
? | |
additional information | high-sensitive electrochemical determination of ethyl carbamate using urethanase and glutamate dehydrogenase modified electrode, with high selectivity during biorecognition between enzymes and the targets, overview | Providencia rettgeri CGMCC 8326 | ? | - |
? | |
urethane + H2O | - |
Providencia rettgeri | ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | - |
Providencia rettgeri JN-B815 | ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | - |
Providencia rettgeri CGMCC 8326 | ethanol + CO2 + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UreC | - |
Providencia rettgeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Providencia rettgeri |