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Information on EC 3.1.21.4 - type II site-specific deoxyribonuclease and Organism(s) Bacillus subtilis and UniProt Accession Q45488
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3.1.21.4 type II site-specific deoxyribonucleaseSpecify your search results
Word Map
- 3.1.21.4
- endonucleases
- phage
- lambda
- agarose
- strand
- rrna
- bacteriophage
-
pcr-rflp
- viruses
-
serotype
-
pulsed-field
-
single-stranded
-
spacers
-
cosmid
-
amplicons
- adenovirus
-
outbreak
- simplex
- herpes
- mtdnas
- cytosine
- duplex
-
serological
-
pcr-based
-
nested
-
epstein-barr
- cpg
-
caucasian
-
methylase
-
reaction-restriction
- herpesvirus
- virion
- satellite
- tetracycline
-
nosocomial
-
32p-labeled
-
proviral
- beta-globin
-
simian
- ethidium
- globin
- ampicillin
-
discriminatory
-
dna-dna
-
unmethylated
-
supercoiled
- serogroups
-
biotype
-
subfragment
- analysis
- biotechnology
- beta-thalassemia
- molecular biology
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Synonyms
restriction enzyme, restriction endonuclease, ecori, hindiii, bamhi, pvuii, haeiii, hpaii, bglii, ecorv, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DNA restriction endonuclease
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DNA restriction enzyme
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-
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EC 3.1.23
-
-
-
-
EC 3.1.24
-
-
-
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Endonuclease AbrI
-
-
-
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Endonuclease AccI
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-
-
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Endonuclease AgeI
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-
-
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Endonuclease ApaLI
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-
-
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Endonuclease AvaI
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-
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Endonuclease BamHI
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-
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Endonuclease BanI
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-
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Endonuclease BglI
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-
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Endonuclease BglII
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-
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Endonuclease BsoBI
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-
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Endonuclease Bsp6I
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-
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Endonuclease BstVI
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-
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Endonuclease BsuBI
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-
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Endonuclease BsuFI
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-
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Endonuclease BsuRI
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-
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Endonuclease CeqI
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-
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Endonuclease Cfr10I
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Endonuclease Cfr9I
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Endonuclease CfrBI
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Endonuclease CviAII
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-
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Endonuclease CviJI
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-
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Endonuclease DdeI
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-
-
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Endonuclease DpnI
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-
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Endonuclease DpnII
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-
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Endonuclease Eco47I
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-
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Endonuclease Eco47II
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-
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Endonuclease EcoRI
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-
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Endonuclease EcoRII
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-
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Endonuclease EcoRV
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-
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Endonuclease FokI
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Endonuclease HaeII
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Endonuclease HaeIII
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Endonuclease HgAI
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Endonuclease HgiBI
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Endonuclease HgiCI
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Endonuclease HgiCII
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-
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Endonuclease HgiDI
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-
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Endonuclease HgiEI
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-
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Endonuclease HgiGI
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-
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Endonuclease HhaII
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-
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Endonuclease HincII
-
-
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Endonuclease HindII
-
-
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Endonuclease HindIII
-
-
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Endonuclease HindVP
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-
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Endonuclease HinfI
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-
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Endonuclease HpaI
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-
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Endonuclease HpaII
-
-
-
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Endonuclease HphI
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-
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Endonuclease KpnI
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-
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Endonuclease LlaDCHI
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Endonuclease MamI
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-
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Endonuclease MboI
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Endonuclease MboII
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Endonuclease MjaI
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Endonuclease MjaII
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-
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Endonuclease MjaIII
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Endonuclease MjaIV
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Endonuclease MjaV
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Endonuclease MjaVIP
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Endonuclease MspI
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Endonuclease MthTI
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Endonuclease MthZI
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-
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Endonuclease MunI
-
-
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Endonuclease MwoI
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-
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Endonuclease NaeI
-
-
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Endonuclease NgoBI
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Endonuclease NgoBV
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Endonuclease NgoFVII
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Endonuclease NgoMIV
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Endonuclease NgoPII
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Endonuclease NlaIII
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Endonuclease NlaIV
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Endonuclease NmeDIP
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Endonuclease NspV
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Endonuclease PaeR7I
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Endonuclease PstI
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Endonuclease PvuI
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Endonuclease PvuII
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Endonuclease RsrI
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-
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Endonuclease SacI
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Endonuclease SalI
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-
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Endonuclease Sau3AI
-
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Endonuclease Sau96I
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Endonuclease ScaI
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Endonuclease ScrFI
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Endonuclease SfiI
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Endonuclease SinI
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Endonuclease SmaI
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Endonuclease SsoII
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Endonuclease StsI
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Endonuclease TaqI
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Endonuclease TthHB8I
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Endonuclease XamI
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Endonuclease XcyI
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LlaII
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nuclease, deoxyribonucleic restriction endo-
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nuclease, restriction endodeoxyribo-
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R.AbrI
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R.AccI
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R.AgeI
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R.ApaLI
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R.AvaI
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R.BamHI
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R.BanI
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R.BglI
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R.BglII
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R.BsoBI
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R.Bsp6I
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R.BstVI
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R.BsuBI
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R.BsuRI
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R.CeqI
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R.Cfr10I
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R.Cfr9I
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R.CfrBI
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R.CviAII
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R.CviJI
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R.DdeI
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R.DpnI
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R.DpnII
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R.Eco47I
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-
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R.Eco47II
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R.EcoRI
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R.EcoRII
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R.EcoRV
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R.FokI
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R.HaeII
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R.HaeIII
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R.HgAI
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R.HgiBI
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R.HgiCI
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R.HgiCII
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R.HgiDI
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R.HgiEI
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R.HgiGI
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R.HhaII
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R.HincII
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R.HindII
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R.HindIII
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R.HindVP
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R.HinfI
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R.HpaI
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R.HpaII
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R.HphI
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R.KpnI
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R.LlaDCHI
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R.MamI
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R.MboI
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R.MboII
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R.MjaI
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R.MjaII
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R.MjaIII
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R.MjaIV
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R.MjaV
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R.MjaVIP
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R.MspI
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R.MthTI
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R.MthZI
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R.MunI
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R.MwoI
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R.NaeI
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R.NgoBI
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R.NgoBV
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R.NgoFVII
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R.NgoI
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R.NgoMIV
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R.NgoPII
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R.NgoV
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R.NgoVII
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R.NlaIII
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R.NlaIV
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R.NmeDIP
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R.NspV
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R.PaeR7I
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R.PstI
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R.PvuI
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R.PvuII
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R.RsrI
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R.SacI
-
-
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R.SalI
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R.Sau3AI
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R.Sau96I
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R.ScaI
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R.ScrFI
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R.SfiI
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R.SinI
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R.SmaI
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R.SsoII
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R.StsI
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R.TaqI
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R.TthHB8I
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R.XamI
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R.XcyI
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restriction endodeoxyribonuclease
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restriction endonuclease
-
-
-
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restriction enzyme
-
-
-
-
type II restriction enzyme
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-08-5
not distinguished from EC 3.1.21.5
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA containing F5dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing F5dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
additional information
?
-
no hydrolysis of DNA containing 5-methyl-dC
-
-
?
DNA + H2O
?
-
primary function is the inactivation of foreign DNA invading bacteria
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
the enzyme recognizes the target DNA sequence 5'CCGG and cleaves between the two cytosines to produce sticky ends with 5'CG overhangs
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BgII: GCCNNNNNGGC. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
-
recognition sequence of BglII: AGATCT. Recognition sites of type II restriction enzymes are underrepresented in host genomes and in phage genomes
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA containing F5dC + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dC
-
-
?
DNA containing F5dU + H2O
?
perfectly hydrolyzes the DNA containing 5-fluoro-dU
-
-
?
additional information
?
-
no hydrolysis of DNA containing 5-methyl-dC
-
-
?
DNA + H2O
?
-
primary function is the inactivation of foreign DNA invading bacteria
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
denatured molecular weights of 33000 Da and 67000 Da are obtained
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the complete restriction -modification system in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roberts, R.J.
Restriction enzymes and their isoschizomers
Nucleic Acids Res.
18
2331-2365
1990
Acetobacter aceti, Gluconobacter oxydans, Acetobacter pasteurianus, Acinetobacter lwoffii, Streptomyces phaeochromogenes, Thalassobius gelatinovorus, Anabaena cylindrica, Dolichospermum flos-aquae, Anabaena subcylindrica, Trichormus variabilis, Synechocystis sp., Aphanothece halophytica, Cellulosimicrobium cellulans, Geobacillus stearothermophilus, Bacillus amyloliquefaciens, Brevibacillus brevis, [Bacillus] caldolyticus, Bacillus cereus, Weizmannia coagulans, Bacillus subtilis, Bacillus pumilus, Lysinibacillus sphaericus, uncultured bacterium, Bifidobacterium longum subsp. infantis, Curtobacterium albidum, Caryophanon latum, Chloroflexus aurantiacus, Chromobacterium violaceum, Citrobacter freundii, Dactylococcus salina, Deinococcus radiodurans, Deinococcus radiophilus, Desulfovibrio desulfuricans, Streptococcus pneumoniae, Escherichia coli, Enterococcus faecalis, Eucapsis sp., Flavobacterium aquatile, Chryseobacterium indologenes, Planomicrobium okeanokoites, Thermus thermophilus, Frankia sp., Fusobacterium nucleatum, Haemophilus aegyptius, Avibacterium paragallinarum, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus parahaemolyticus, Haemophilus parainfluenzae, Herpetosiphon aurantiacus, Klebsiella pneumoniae, Methanothermobacter wolfeii, Methanococcus aeolicus, Methylophilus methylotrophus, Psychrobacter urativorans, Micrococcus luteus, Micrococcus lylae, Staphylococcus aureus, Microcoleus sp., Moraxella bovis, Moraxella nonliquefaciens, Mycoplasmopsis fermentans, Bergeriella denitrificans, Neisseria lactamica, Neisseria mucosa heidelbergensis, Lentzea aerocolonigenes, Nocardia argentinensis, Gordonia rubripertincta, Nocardia otitidiscaviarum, Rhodococcus ruber, Nostoc sp., Plesiomonas shigelloides, Proteus vulgaris, Pseudomonas sp., Pseudomonas putida, Pseudomonas fluorescens, Paucimonas lemoignei, Stenotrophomonas maltophilia, Pseudomonas stutzeri, Rhizobium leguminosarum, Cereibacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhi, Serratia marcescens, Sphaerotilus natans, Sphaerotilus sp., Arthrospira platensis, Streptomyces achromogenes, Lactococcus cremoris, Enterococcus durans, Streptomyces albus, Streptomyces caespitosus, Streptomyces fimbriatus, Thermus aquaticus, Thermus filiformis, Thermus sp., Vibrio sp., Xanthomonas campestris pv. badrii, Xanthomonas campestris, Xanthomonas phaseoli pv. manihotis, Xanthomonas vasicola, Xanthomonas citri pv. malvacearum, Acetobacter pasteurianus ApaLI
Kapfer, W.; Walter, J.; Trautner, T.A.
Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme
Nucleic Acids Res.
19
6457-6463
1991
Bacillus subtilis
Wells, R.D.; Klein, R.D.; Singleton, C.K.
Type II restriction enzymes
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
14
157-191
1981
Trichormus variabilis, Cellulosimicrobium cellulans, Geobacillus stearothermophilus, Bacillus amyloliquefaciens, [Bacillus] caldolyticus, Bacillus subtilis, Lysinibacillus sphaericus, Desulfovibrio desulfuricans, Streptococcus pneumoniae, Escherichia coli, Enterococcus faecalis, Thermus thermophilus, Fusobacterium nucleatum, Haemophilus aegyptius, Avibacterium paragallinarum, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus parahaemolyticus, Haemophilus parainfluenzae, Herpetosiphon aurantiacus, Staphylococcus aureus, Moraxella bovis, Providencia stuartii, Cereibacter sphaeroides, Serratia marcescens, Streptomyces achromogenes, Streptomyces albus, Xanthomonas campestris pv. badrii, Xanthomonas vasicola, Xanthomonas citri pv. malvacearum, Staphylococcus aureus Sau96I
-
Wilson, G.G.
Type II restriction-modification systems
Trends Genet.
4
314-318
1988
Dolichospermum flos-aquae, Trichormus variabilis, Bacillus amyloliquefaciens, Bacillus subtilis, Desulfovibrio desulfuricans, Streptococcus pneumoniae, Escherichia coli, Planomicrobium okeanokoites, Haemophilus aegyptius, Haemophilus haemolyticus, Haemophilus influenzae, Herpetosiphon aurantiacus, Methanothermobacter wolfeii, Proteus vulgaris, Providencia stuartii, Streptomyces albus, Thermus aquaticus, Xanthomonas campestris pv. badrii
Kiss, A.; Posfai, G.; Keller, C.C.; Venetianer, P.; Roberts, R.J.
Nucleotide sequence of the BsuRI restriction-modification system
Nucleic Acids Res.
13
6403-6420
1985
Bacillus subtilis
Jutur, P.P.; Reddy, A.R.
Isolation and partial purification of a novel type II restriction endonuclease Bsu121 I, from Bacillus subtilis. Bsu121I, a type II restriction endonuclease from Bacillus subtilis
Mol. Biol. Rep.
29
383-385
2002
Bacillus subtilis
Jutur, P.P.; Hoti, S.L.; Reddy, A.R.
Bsu2413I and Bfi2411I, two new thermophilic type II restriction endonucleases from Bacillus subtilis and Bacillus firmus: isolation and partial purification. Thermophilic endonucleases from two Bacillus species
Mol. Biol. Rep.
31
139-142
2004
Cytobacillus firmus, Bacillus subtilis, Cytobacillus firmus 2411, Bacillus subtilis 2413
Nikolajewa, S.; Beyer, A.; Friedel, M.; Hollunder, J.; Wilhelm, T.
Common patterns in type II restriction enzyme binding sites
Nucleic Acids Res.
33
2726-2733
2005
Geobacillus stearothermophilus, Bacillus amyloliquefaciens, Bacillus subtilis, Citrobacter freundii, Escherichia coli, Planomicrobium okeanokoites, Haemophilus influenzae, Moraxella sp., Mycoplasma sp., Neisseria gonorrhoeae, Lentzea aerocolonigenes, Proteus vulgaris
Olszewska, A.; Dadova, J.; Mackova, M.; Hocek, M.
Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases
Bioorg. Med. Chem.
23
6885-6890
2015
Dolichospermum flos-aquae (E3VX87), Streptomyces caespitosus (O52691), Acetobacter pasteurianus (O52703), Gordonia rubripertincta (O85489), Pyrococcus sp. GI-H (O93646), Providencia stuartii (P00640), Escherichia coli (P00642), Proteus hauseri (P23657), Bacillus amyloliquefaciens (P23940), Klebsiella pneumoniae (P25237), Haemophilus influenzae (P43870), Bacillus subtilis (Q45488), Acidithiobacillus ferrooxidans (Q4GZN8), Fuscovulum blasticum (Q6SA27), Dolichospermum flos-aquae CCAP 1403/13F (E3VX87), Haemophilus influenzae ATCC 51907 (P43870)
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