Any feedback?
Literature summary for 3.1.21.4 extracted from
- Type II restriction enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 157-191.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
ColE1 DNA | EcoRI, activated by Co2+, 37°C | Escherichia coli | |
| 0.003 | 0.008 | ColE1 DNA | EcoRI, activated by Mg2+ | Escherichia coli | |
| 0.005 | - |
pBR322 DNA | EcoRI, 37°C | Escherichia coli | |
| 0.01 | - |
Lambda DNA | EcoRI, 37°C | Escherichia coli | |
| 0.03 | - |
SV40 DNA | EcoRI, 37°C | Escherichia coli | |
| 0.03 | - |
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) | - |
Haemophilus parainfluenzae |  |
| 0.03 | - |
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) | Hpa, 25°C | Bacillus amyloliquefaciens | |
| 0.18 | - |
d(G-G-T-T-A-A-C-C) | HpaI, 25°C | Haemophilus parainfluenzae | |
| 0.3 | 0.36 | pJC80 DNA | BamHI, 37°C | Bacillus amyloliquefaciens | |
| 0.9 | - |
pJC linearized plasmid DNA | BamHI, 37°C | Bacillus amyloliquefaciens | |
| 7 | - |
d(pT-G-A-A-T-T-C-A) | EcoR1, 12 C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can replace for Mg2+ | Escherichia coli | |
| Mg2+ | required | Avibacterium paragallinarum | |
| Mg2+ | required | Bacillus amyloliquefaciens | |
| Mg2+ | required | Bacillus subtilis | |
| Mg2+ | required | Cellulosimicrobium cellulans | |
| Mg2+ | required | Cereibacter sphaeroides | |
| Mg2+ | required | Desulfovibrio desulfuricans | |
| Mg2+ | required | Enterococcus faecalis | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Fusobacterium nucleatum | |
| Mg2+ | required | Geobacillus stearothermophilus | |
| Mg2+ | required | Haemophilus aegyptius | |
| Mg2+ | required | Haemophilus haemolyticus | |
| Mg2+ | required | Haemophilus influenzae | |
| Mg2+ | required | Haemophilus parahaemolyticus | |
| Mg2+ | required | Haemophilus parainfluenzae | |
| Mg2+ | required | Herpetosiphon aurantiacus | |
| Mg2+ | required | Lysinibacillus sphaericus | |
| Mg2+ | optimal concentration is 20 mM | Lysinibacillus sphaericus | |
| Mg2+ | required | Moraxella bovis | |
| Mg2+ | required | Providencia stuartii | |
| Mg2+ | required | Serratia marcescens | |
| Mg2+ | required | Staphylococcus aureus | |
| Mg2+ | required | Streptococcus pneumoniae | |
| Mg2+ | required | Streptomyces albus | |
| Mg2+ | required | Thermus thermophilus | |
| Mg2+ | required | Trichormus variabilis | |
| Mg2+ | required | Xanthomonas campestris pv. badrii | |
| Mg2+ | required | Xanthomonas citri pv. malvacearum | |
| Mg2+ | required | Xanthomonas vasicola | |
| Mg2+ | required | [Bacillus] caldolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
enzyme DpnI | Streptococcus pneumoniae |
| 22000 | - |
enzyme BamHI | Bacillus amyloliquefaciens |
| 24000 | - |
enzyme HhaII | Haemophilus haemolyticus |
| 25000 | - |
enzyme BclI | [Bacillus] caldolyticus |
| 26000 | - |
enzyme BstI | Geobacillus stearothermophilus |
| 27000 | - |
enzyme BglII | Bacillus subtilis |
| 28500 | - |
enzyme EcoRI | Escherichia coli |
| 31000 | - |
enzyme BglI | Bacillus subtilis |
| 40000 | - |
enzyme HpaII | Haemophilus parainfluenzae |
| 68000 | - |
enzyme BsuI | Bacillus subtilis |
| 70000 | - |
enzyme HindII | Haemophilus influenzae |
| 70000 | - |
enzyme DpnII | Streptococcus pneumoniae |
| 95000 | - |
enzyme Tth111II | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avibacterium paragallinarum | - |
- |
- |
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Cellulosimicrobium cellulans | - |
- |
- |
| Cereibacter sphaeroides | - |
- |
- |
| Desulfovibrio desulfuricans | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Fusobacterium nucleatum | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Haemophilus aegyptius | - |
- |
- |
| Haemophilus haemolyticus | - |
enzyme HhaII | - |
| Haemophilus influenzae | - |
- |
- |
| Haemophilus parahaemolyticus | - |
enzyme HphI | - |
| Haemophilus parainfluenzae | - |
enzyme HpaII | - |
| Haemophilus parainfluenzae | - |
enzymes HpaI | - |
| Herpetosiphon aurantiacus | - |
- |
- |
| Lysinibacillus sphaericus | - |
- |
- |
| Moraxella bovis | - |
enzymes MboI and MboII | - |
| Providencia stuartii | - |
- |
- |
| Serratia marcescens | - |
- |
- |
| Staphylococcus aureus | - |
Sau96I | - |
| Staphylococcus aureus Sau96I | - |
Sau96I | - |
| Streptococcus pneumoniae | - |
enzyme Dpn II | - |
| Streptococcus pneumoniae | - |
enzyme DpnI | - |
| Streptomyces achromogenes | - |
- |
- |
| Streptomyces albus | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
| Trichormus variabilis | - |
- |
- |
| Xanthomonas campestris pv. badrii | - |
enzyme XbaI | - |
| Xanthomonas citri pv. malvacearum | - |
- |
- |
| Xanthomonas vasicola | - |
- |
- |
| [Bacillus] caldolyticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ColE1 DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O | - |
Haemophilus parainfluenzae | ? | - |
? | |
| d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| d(G-G-T-T-5'-bromodeoxyuridine-C-C) + H2O | - |
Haemophilus parainfluenzae | ? | - |
? | |
| d(G-G-T-T-A-A-C-C) + H2O | - |
Haemophilus parainfluenzae | ? | - |
? | |
| d(pT-G-A-A-T-T-C-A) + H2O | - |
Escherichia coli | ? | - |
? | |
| DNA + H2O | - |
Staphylococcus aureus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Haemophilus influenzae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Bacillus subtilis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Thermus thermophilus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Geobacillus stearothermophilus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Cereibacter sphaeroides | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Lysinibacillus sphaericus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Serratia marcescens | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Enterococcus faecalis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Bacillus amyloliquefaciens | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Streptomyces albus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
[Bacillus] caldolyticus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Fusobacterium nucleatum | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Avibacterium paragallinarum | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Desulfovibrio desulfuricans | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Trichormus variabilis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Haemophilus aegyptius | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Haemophilus parahaemolyticus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Xanthomonas citri pv. malvacearum | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Streptococcus pneumoniae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Providencia stuartii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Cellulosimicrobium cellulans | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Xanthomonas vasicola | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Xanthomonas campestris pv. badrii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Herpetosiphon aurantiacus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Haemophilus haemolyticus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Haemophilus parainfluenzae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Moraxella bovis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Streptomyces achromogenes | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | ColE1 DNA | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | pBR322 DNA | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | SV40 DNA | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | lambda DNA | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| DNA + H2O | - |
Staphylococcus aureus Sau96I | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| lambda DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| NTP14 DNA + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| pBR322 DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| pJC linearized plasmid DNA + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| pJC80 DNA + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| SV40 DNA + H2O | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AluI | - |
Cellulosimicrobium cellulans |
| BglI | - |
Bacillus subtilis |
| BglII | - |
Bacillus subtilis |
| DpnI | - |
Streptococcus pneumoniae |
| DpnII | - |
Streptococcus pneumoniae |
| HgiAI | - |
Herpetosiphon aurantiacus |
| HindII | - |
Haemophilus influenzae |
| HindIII | - |
Haemophilus influenzae |
| HinfI | - |
Haemophilus influenzae |
| PstI | - |
Providencia stuartii |
| RsaI | - |
Cereibacter sphaeroides |
| sau96I | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | SmaI | Serratia marcescens |
| 25 | - |
XmaIII | Xanthomonas citri pv. malvacearum |
| 30 | 40 | BglI and BglII | Bacillus subtilis |
| 30 | 45 | HgiAI | Herpetosiphon aurantiacus |
| 30 | - |
Sau96I and Sau3AI | Staphylococcus aureus |
| 34 | - |
RsaI | Cereibacter sphaeroides |
| 37 | - |
HgaI | Avibacterium paragallinarum |
| 37 | - |
BamHI | Bacillus amyloliquefaciens |
| 37 | - |
AluI | Cellulosimicrobium cellulans |
| 37 | - |
DdeI | Desulfovibrio desulfuricans |
| 37 | - |
Sfa | Enterococcus faecalis |
| 37 | - |
EcoRI | Escherichia coli |
| 37 | - |
FnuEI, FnuHI and FnuDII | Fusobacterium nucleatum |
| 37 | - |
HaeII | Haemophilus aegyptius |
| 37 | - |
HhaI and HhaII | Haemophilus haemolyticus |
| 37 | - |
HinfI, Hind II and HindIII | Haemophilus influenzae |
| 37 | - |
HphI | Haemophilus parahaemolyticus |
| 37 | - |
HpaII | Haemophilus parainfluenzae |
| 37 | - |
Bsp | Lysinibacillus sphaericus |
| 37 | - |
MboI and MboII | Moraxella bovis |
| 37 | - |
PstI | Providencia stuartii |
| 37 | - |
DpnI, DPNII | Streptococcus pneumoniae |
| 37 | - |
SalI and SalPI | Streptomyces albus |
| 37 | - |
Ava I and AvaII | Trichormus variabilis |
| 37 | - |
XbaI | Xanthomonas campestris pv. badrii |
| 37 | - |
XmaI and XmaII | Xanthomonas citri pv. malvacearum |
| 37 | - |
XhoI | Xanthomonas vasicola |
| 45 | - |
HpaI | Haemophilus parainfluenzae |
| 50 | - |
BclI | [Bacillus] caldolyticus |
| 55 | - |
BstPI | Streptomyces achromogenes |
| 60 | 65 | BstI | Geobacillus stearothermophilus |
| 60 | 70 | Tth111I | Thermus thermophilus |
| 65 | 70 | Tth111II | Thermus thermophilus |
| 70 | - |
HaeIII | Haemophilus aegyptius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0167 | - |
ColE1 DNA | EcoRI, activated by Co2+, 37°C | Escherichia coli | |
| 0.0217 | - |
Lambda DNA | EcoR1, 37°C | Escherichia coli | |
| 0.025 | - |
SV40 DNA | EcoR1, 37°C | Escherichia coli | |
| 0.0267 | - |
NTP14 DNA | BamHI, 37°C | Bacillus amyloliquefaciens | |
| 0.03 | - |
pBR322 DNA | EcoRI, pBR322, 37°C | Escherichia coli | |
| 0.0367 | - |
pJC80 DNA | BamHI, 37°C | Bacillus amyloliquefaciens | |
| 0.0667 | - |
d(pT-G-A-A-T-T-C-A) | EcoRI, 12°C | Escherichia coli | |
| 0.0667 | 0.133 | ColE1 DNA | EcoRI, activated by Mg2+ | Escherichia coli | |
| 600 | - |
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) | - |
Haemophilus parainfluenzae | |
| 600 | - |
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) | Hpa, 25°C | Bacillus amyloliquefaciens | |
| 2830 | - |
d(G-G-T-T-A-A-C-C) | HpaI, 25°C | Haemophilus parainfluenzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
HpaII | Haemophilus parainfluenzae |
| 7.1 | 7.5 | EcoRI | Escherichia coli |
| 7.4 | - |
PstI | Providencia stuartii |
| 7.4 | - |
Sau96I | Staphylococcus aureus |
| 7.4 | - |
HphI | Haemophilus parahaemolyticus |
| 7.4 | - |
Tth111I | Thermus thermophilus |
| 7.4 | - |
HaeII | Haemophilus aegyptius |
| 7.4 | - |
HhaI | Haemophilus haemolyticus |
| 7.4 | - |
BclI | [Bacillus] caldolyticus |
| 7.5 | - |
HinfI | Haemophilus influenzae |
| 7.5 | - |
Tth111II | Thermus thermophilus |
| 7.5 | - |
HaeIII | Haemophilus aegyptius |
| 7.5 | - |
Sau3AI | Staphylococcus aureus |
| 7.5 | - |
XmaI | Xanthomonas citri pv. malvacearum |
| 7.5 | - |
Sfa | Enterococcus faecalis |
| 7.5 | - |
SmaI | Serratia marcescens |
| 7.5 | - |
DdeI | Desulfovibrio desulfuricans |
| 7.5 | 8 | HindII | Haemophilus influenzae |
| 7.5 | 8 | BstI | Geobacillus stearothermophilus |
| 7.5 | 8.5 | HgiAI | Herpetosiphon aurantiacus |
| 7.5 | 8.8 | AvaI | Trichormus variabilis |
| 7.5 | 9 | AvaII | Trichormus variabilis |
| 7.6 | - |
DpnI and DpnII | Streptococcus pneumoniae |
| 7.7 | 8.1 | HpaI | Haemophilus parainfluenzae |
| 7.9 | - |
AluI | Cellulosimicrobium cellulans |
| 7.9 | - |
MboI and MboII | Moraxella bovis |
| 7.9 | - |
SalI and SalPI | Streptomyces albus |
| 7.9 | - |
XmaII | Xanthomonas citri pv. malvacearum |
| 7.9 | - |
HgaI | Avibacterium paragallinarum |
| 7.9 | - |
RsaI | Cereibacter sphaeroides |
| 7.9 | - |
Fnu4HI, FnuDII and FnuEI | Fusobacterium nucleatum |
| 7.9 | - |
XhoI | Xanthomonas vasicola |
| 7.9 | - |
XbaI | Xanthomonas campestris pv. badrii |
| 8 | 9 | HindIII | Haemophilus influenzae |
| 8 | 8.5 | Bsp | Lysinibacillus sphaericus |
| 8.2 | - |
- |
Lysinibacillus sphaericus |
| 8.2 | - |
XmaIII | Xanthomonas citri pv. malvacearum |
| 8.5 | - |
BamHI | Bacillus amyloliquefaciens |
| 9 | - |
HhaII | Haemophilus haemolyticus |
| 9.5 | - |
BglI and BglII | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
