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Literature summary for 3.1.21.4 extracted from

  • Wells, R.D.; Klein, R.D.; Singleton, C.K.
    Type II restriction enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 157-191.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
ColE1 DNA EcoRI, activated by Co2+, 37°C Escherichia coli
0.003 0.008 ColE1 DNA EcoRI, activated by Mg2+ Escherichia coli
0.005
-
pBR322 DNA EcoRI, 37°C Escherichia coli
0.01
-
Lambda DNA EcoRI, 37°C Escherichia coli
0.03
-
d(G-G-T-5-bromodeoxyuridine-A-A-C-C)
-
Haemophilus parainfluenzae
0.03
-
SV40 DNA EcoRI, 37°C Escherichia coli
0.03
-
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) Hpa, 25°C Bacillus amyloliquefaciens
0.18
-
d(G-G-T-T-A-A-C-C) HpaI, 25°C Haemophilus parainfluenzae
0.3 0.36 pJC80 DNA BamHI, 37°C Bacillus amyloliquefaciens
0.9
-
pJC linearized plasmid DNA BamHI, 37°C Bacillus amyloliquefaciens
7
-
d(pT-G-A-A-T-T-C-A) EcoR1, 12 C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace for Mg2+ Escherichia coli
Mg2+ required Staphylococcus aureus
Mg2+ required Haemophilus influenzae
Mg2+ required Bacillus subtilis
Mg2+ required Thermus thermophilus
Mg2+ required Escherichia coli
Mg2+ required Geobacillus stearothermophilus
Mg2+ required Cereibacter sphaeroides
Mg2+ required Lysinibacillus sphaericus
Mg2+ required Serratia marcescens
Mg2+ required Enterococcus faecalis
Mg2+ required Streptococcus pneumoniae
Mg2+ required Bacillus amyloliquefaciens
Mg2+ required Streptomyces albus
Mg2+ required [Bacillus] caldolyticus
Mg2+ required Fusobacterium nucleatum
Mg2+ required Avibacterium paragallinarum
Mg2+ required Desulfovibrio desulfuricans
Mg2+ required Trichormus variabilis
Mg2+ required Haemophilus aegyptius
Mg2+ required Haemophilus parahaemolyticus
Mg2+ required Xanthomonas citri pv. malvacearum
Mg2+ required Providencia stuartii
Mg2+ required Cellulosimicrobium cellulans
Mg2+ required Xanthomonas vasicola
Mg2+ required Xanthomonas campestris pv. badrii
Mg2+ required Herpetosiphon aurantiacus
Mg2+ required Haemophilus haemolyticus
Mg2+ required Haemophilus parainfluenzae
Mg2+ required Moraxella bovis
Mg2+ optimal concentration is 20 mM Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
enzyme DpnI Streptococcus pneumoniae
22000
-
enzyme BamHI Bacillus amyloliquefaciens
24000
-
enzyme HhaII Haemophilus haemolyticus
25000
-
enzyme BclI [Bacillus] caldolyticus
26000
-
enzyme BstI Geobacillus stearothermophilus
27000
-
enzyme BglII Bacillus subtilis
28500
-
enzyme EcoRI Escherichia coli
31000
-
enzyme BglI Bacillus subtilis
40000
-
enzyme HpaII Haemophilus parainfluenzae
68000
-
enzyme BsuI Bacillus subtilis
70000
-
enzyme HindII Haemophilus influenzae
70000
-
enzyme DpnII Streptococcus pneumoniae
95000
-
enzyme Tth111II Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Avibacterium paragallinarum
-
-
-
Bacillus amyloliquefaciens
-
-
-
Bacillus subtilis
-
-
-
Cellulosimicrobium cellulans
-
-
-
Cereibacter sphaeroides
-
-
-
Desulfovibrio desulfuricans
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Fusobacterium nucleatum
-
-
-
Geobacillus stearothermophilus
-
-
-
Haemophilus aegyptius
-
-
-
Haemophilus haemolyticus
-
enzyme HhaII
-
Haemophilus influenzae
-
-
-
Haemophilus parahaemolyticus
-
enzyme HphI
-
Haemophilus parainfluenzae
-
enzyme HpaII
-
Haemophilus parainfluenzae
-
enzymes HpaI
-
Herpetosiphon aurantiacus
-
-
-
Lysinibacillus sphaericus
-
-
-
Moraxella bovis
-
enzymes MboI and MboII
-
Providencia stuartii
-
-
-
Serratia marcescens
-
-
-
Staphylococcus aureus
-
Sau96I
-
Staphylococcus aureus Sau96I
-
Sau96I
-
Streptococcus pneumoniae
-
enzyme Dpn II
-
Streptococcus pneumoniae
-
enzyme DpnI
-
Streptomyces achromogenes
-
-
-
Streptomyces albus
-
-
-
Thermus thermophilus
-
-
-
Trichormus variabilis
-
-
-
Xanthomonas campestris pv. badrii
-
enzyme XbaI
-
Xanthomonas citri pv. malvacearum
-
-
-
Xanthomonas vasicola
-
-
-
[Bacillus] caldolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ColE1 DNA + H2O
-
Escherichia coli ?
-
?
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O
-
Bacillus amyloliquefaciens ?
-
?
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O
-
Haemophilus parainfluenzae ?
-
?
d(G-G-T-T-5'-bromodeoxyuridine-C-C) + H2O
-
Haemophilus parainfluenzae ?
-
?
d(G-G-T-T-A-A-C-C) + H2O
-
Haemophilus parainfluenzae ?
-
?
d(pT-G-A-A-T-T-C-A) + H2O
-
Escherichia coli ?
-
?
DNA + H2O
-
Staphylococcus aureus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Haemophilus influenzae double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Bacillus subtilis double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Thermus thermophilus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Geobacillus stearothermophilus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Cereibacter sphaeroides double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Lysinibacillus sphaericus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Serratia marcescens double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Enterococcus faecalis double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Streptococcus pneumoniae double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Bacillus amyloliquefaciens double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Streptomyces albus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Streptomyces achromogenes double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
[Bacillus] caldolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Fusobacterium nucleatum double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Avibacterium paragallinarum double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Desulfovibrio desulfuricans double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Trichormus variabilis double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Haemophilus aegyptius double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Haemophilus parahaemolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Xanthomonas citri pv. malvacearum double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Providencia stuartii double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Cellulosimicrobium cellulans double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Xanthomonas vasicola double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Xanthomonas campestris pv. badrii double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Herpetosiphon aurantiacus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Haemophilus haemolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Haemophilus parainfluenzae double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Moraxella bovis double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O ColE1 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O pBR322 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O SV40 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O lambda DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
?
DNA + H2O
-
Staphylococcus aureus Sau96I double-stranded DNA fragments with terminal 5'-phosphates
-
?
lambda DNA + H2O
-
Escherichia coli ?
-
?
NTP14 DNA + H2O
-
Bacillus amyloliquefaciens ?
-
?
pBR322 DNA + H2O
-
Escherichia coli ?
-
?
pJC linearized plasmid DNA + H2O
-
Bacillus amyloliquefaciens ?
-
?
pJC80 DNA + H2O
-
Bacillus amyloliquefaciens ?
-
?
SV40 DNA + H2O
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
AluI
-
Cellulosimicrobium cellulans
BglI
-
Bacillus subtilis
BglII
-
Bacillus subtilis
DpnI
-
Streptococcus pneumoniae
DpnII
-
Streptococcus pneumoniae
HgiAI
-
Herpetosiphon aurantiacus
HindII
-
Haemophilus influenzae
HindIII
-
Haemophilus influenzae
HinfI
-
Haemophilus influenzae
PstI
-
Providencia stuartii
RsaI
-
Cereibacter sphaeroides
sau96I
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
XmaIII Xanthomonas citri pv. malvacearum
25 37 SmaI Serratia marcescens
30
-
Sau96I and Sau3AI Staphylococcus aureus
30 40 BglI and BglII Bacillus subtilis
30 45 HgiAI Herpetosiphon aurantiacus
34
-
RsaI Cereibacter sphaeroides
37
-
HaeII Haemophilus aegyptius
37
-
XhoI Xanthomonas vasicola
37
-
SalI and SalPI Streptomyces albus
37
-
DdeI Desulfovibrio desulfuricans
37
-
MboI and MboII Moraxella bovis
37
-
Sfa Enterococcus faecalis
37
-
FnuEI, FnuHI and FnuDII Fusobacterium nucleatum
37
-
HpaII Haemophilus parainfluenzae
37
-
XmaI and XmaII Xanthomonas citri pv. malvacearum
37
-
HphI Haemophilus parahaemolyticus
37
-
XbaI Xanthomonas campestris pv. badrii
37
-
HgaI Avibacterium paragallinarum
37
-
HinfI, Hind II and HindIII Haemophilus influenzae
37
-
HhaI and HhaII Haemophilus haemolyticus
37
-
DpnI, DPNII Streptococcus pneumoniae
37
-
Ava I and AvaII Trichormus variabilis
37
-
AluI Cellulosimicrobium cellulans
37
-
PstI Providencia stuartii
37
-
Bsp Lysinibacillus sphaericus
37
-
EcoRI Escherichia coli
37
-
BamHI Bacillus amyloliquefaciens
45
-
HpaI Haemophilus parainfluenzae
50
-
BclI [Bacillus] caldolyticus
55
-
BstPI Streptomyces achromogenes
60 65 BstI Geobacillus stearothermophilus
60 70 Tth111I Thermus thermophilus
65 70 Tth111II Thermus thermophilus
70
-
HaeIII Haemophilus aegyptius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0167
-
ColE1 DNA EcoRI, activated by Co2+, 37°C Escherichia coli
0.0217
-
Lambda DNA EcoR1, 37°C Escherichia coli
0.025
-
SV40 DNA EcoR1, 37°C Escherichia coli
0.0267
-
NTP14 DNA BamHI, 37°C Bacillus amyloliquefaciens
0.03
-
pBR322 DNA EcoRI, pBR322, 37°C Escherichia coli
0.0367
-
pJC80 DNA BamHI, 37°C Bacillus amyloliquefaciens
0.0667
-
d(pT-G-A-A-T-T-C-A) EcoRI, 12°C Escherichia coli
0.0667 0.133 ColE1 DNA EcoRI, activated by Mg2+ Escherichia coli
600
-
d(G-G-T-5-bromodeoxyuridine-A-A-C-C)
-
Haemophilus parainfluenzae
600
-
d(G-G-T-5-bromodeoxyuridine-A-A-C-C) Hpa, 25°C Bacillus amyloliquefaciens
2830
-
d(G-G-T-T-A-A-C-C) HpaI, 25°C Haemophilus parainfluenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
HpaII Haemophilus parainfluenzae
7.1 7.5 EcoRI Escherichia coli
7.4
-
PstI Providencia stuartii
7.4
-
Sau96I Staphylococcus aureus
7.4
-
HphI Haemophilus parahaemolyticus
7.4
-
Tth111I Thermus thermophilus
7.4
-
HaeII Haemophilus aegyptius
7.4
-
HhaI Haemophilus haemolyticus
7.4
-
BclI [Bacillus] caldolyticus
7.5 9 AvaII Trichormus variabilis
7.5
-
HinfI Haemophilus influenzae
7.5
-
Tth111II Thermus thermophilus
7.5
-
HaeIII Haemophilus aegyptius
7.5
-
Sau3AI Staphylococcus aureus
7.5
-
XmaI Xanthomonas citri pv. malvacearum
7.5
-
Sfa Enterococcus faecalis
7.5
-
SmaI Serratia marcescens
7.5
-
DdeI Desulfovibrio desulfuricans
7.5 8.5 HgiAI Herpetosiphon aurantiacus
7.5 8 HindII Haemophilus influenzae
7.5 8 BstI Geobacillus stearothermophilus
7.5 8.8 AvaI Trichormus variabilis
7.6
-
DpnI and DpnII Streptococcus pneumoniae
7.7 8.1 HpaI Haemophilus parainfluenzae
7.9
-
AluI Cellulosimicrobium cellulans
7.9
-
MboI and MboII Moraxella bovis
7.9
-
SalI and SalPI Streptomyces albus
7.9
-
XmaII Xanthomonas citri pv. malvacearum
7.9
-
HgaI Avibacterium paragallinarum
7.9
-
RsaI Cereibacter sphaeroides
7.9
-
Fnu4HI, FnuDII and FnuEI Fusobacterium nucleatum
7.9
-
XhoI Xanthomonas vasicola
7.9
-
XbaI Xanthomonas campestris pv. badrii
8 8.5 Bsp Lysinibacillus sphaericus
8 9 HindIII Haemophilus influenzae
8.2
-
-
Lysinibacillus sphaericus
8.2
-
XmaIII Xanthomonas citri pv. malvacearum
8.5
-
BamHI Bacillus amyloliquefaciens
9
-
HhaII Haemophilus haemolyticus
9.5
-
BglI and BglII Bacillus subtilis