EC Number |
Natural Substrates |
---|
3.1.21.4 | DNA + H2O |
- |
3.1.21.4 | DNA + H2O |
primary function is the inactivation of foreign DNA invading bacteria |
3.1.21.4 | DNA + H2O |
sequence-specific endonucleolytic digestion of infecting DNA |
3.1.21.4 | DNA containing F5dC + H2O |
perfectly hydrolyzes the DNA containing 5-fluoro-dC |
3.1.21.4 | DNA containing F5dU + H2O |
perfectly hydrolyzes the DNA containing 5-fluoro-dU |
3.1.21.4 | double-stranded DNA + H2O |
- |
3.1.21.4 | double-stranded DNA + H2O |
cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC |
3.1.21.4 | double-stranded DNA + H2O |
cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC |
3.1.21.4 | double-stranded DNA + H2O |
cleavage by HindII is blunt, producing fragments with flush ends |
3.1.21.4 | double-stranded DNA + H2O |
EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively |