EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.21.4 | Ca2+ |
in the presence of Ca2+ ions, EcoRII binds to the target DNA but does not cleave the DNA strand |
693058 |
| 3.1.21.4 | Ca2+ |
may substitute for Mg2+, as effective as Mg2+ |
651363 |
| 3.1.21.4 | Ca2+ |
site-specific binding of enzyme to DNA in presence of Ca2+, without the catalytic cleavage. Binding kinetic parameters in presence of Ca2+ are consistent with those in presence of Mg2+ |
683067 |
| 3.1.21.4 | Co2+ |
can replace for Mg2+ |
134262 |
| 3.1.21.4 | Co2+ |
can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations |
679610 |
| 3.1.21.4 | Co2+ |
may substitute for Mg2+, much less effective, relaxed specificity of enzyme |
651363 |
| 3.1.21.4 | K+ |
10-30 mM required for maximal activity |
679808 |
| 3.1.21.4 | Mg2+ |
dependent on |
729268 |
| 3.1.21.4 | Mg2+ |
EcoRV binds two magnesium ions in the active site. One of these ions, Mg2+A, binds to the phosphate group where the cleavage occurs and is required for catalysis. The other, Mg2+B, is crucial for achieving a tightly bound protein-DNA complex and stabilizing a conformation that allows cleavage, structure analysis, molecular dynamics simulations, overview |
715039 |
| 3.1.21.4 | Mg2+ |
highest activity at 5-7 mM |
679610 |
