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Literature summary for 3.1.21.4 extracted from

  • Pollak, A.J.; Chin, A.T.; Reich, N.O.
    Distinct facilitated diffusion mechanisms by E. coli Type II restriction endonucleases (2014), Biochemistry, 53, 7028-7037.
    View publication on PubMed
Search for more literature for 3.1.21.4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
additional information Escherichia coli EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
additional information EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
EcoRI
-
 Escherichia coli
EcoRV
-
 Escherichia coli
type II ENase
-
 Escherichia coli
type II restriction enonuclease
-
 Escherichia coli