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Literature summary for 3.1.21.4 extracted from

  • Matvienko, N.I.; Kramarov, V.M.; Pachkunov, D.M.
    Isolation and some properties of the site-specific endonuclease and methylase Bme2161 from Bacillus megaterium 216 (1987), Eur. J. Biochem., 165, 565-570.
    View publication on PubMed
Search for more literature for 3.1.21.4

Inhibitors

Inhibitors Comment Organism Structure
Co2+ complete inhibition at 1 mM Priestia megaterium
Mn2+ complete inhibition at 3 mM Priestia megaterium
NaCl inhibitory above 0.2 mM Priestia megaterium
Ni2+ complete inhibition at 1 mM Priestia megaterium
Zn2+
-
 Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations Priestia megaterium
Mg2+ highest activity at 5-7 mM Priestia megaterium
Mn2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.3 mM, inhibitory at high concentrations Priestia megaterium
Ni2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE, native mass by gel filtration Priestia megaterium
60000
-
 gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Priestia megaterium recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands ? sticky end fragments ?
dsDNA + H2O Priestia megaterium 216 recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands ? sticky end fragments ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium 216
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium

Storage Stability

Storage Stability Organism
-20°C, 10 mM phosphate buffer, pH 7.4, 0.1 mM EDTA, 2 mM dithiothreitol, 0.2 M NaCl, 50% glycerol, fully stable for at least 1 year Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands Priestia megaterium ? sticky end fragments ?
dsDNA + H2O recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands Priestia megaterium 216 ? sticky end fragments ?
pBR322 DNA + H2O
-
 Priestia megaterium ?
-
 ?
pBR322 DNA + H2O
-
 Priestia megaterium 216 ?
-
 ?

Subunits

Subunits Comment Organism
dimer x * 30000, SDS-PAGE, native mass by gel filtration Priestia megaterium

Synonyms

Synonyms Comment Organism
site-specific endonuclease Bme216I recognition site is identical with the endonuclease AvaII from Anabaena variabilis Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Priestia megaterium