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Literature summary for 3.1.21.4 extracted from

  • Prangishvili, D.A.; Vashakidze, R.P.; Chelidze, M.G.; Gabriadze I.Y.
    A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. (1985), FEBS Lett., 192, 57-60.
    View publication on PubMed
Search for more literature for 3.1.21.4

Inhibitors

Inhibitors Comment Organism Structure
K+ above 100 mM, significant inhibition Sulfolobus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Sulfolobus acidocaldarius
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10-30 mM required for maximal activity Sulfolobus acidocaldarius
Mg2+ required, optimal concentration: 5 mM Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius
-
 isoform SuaI, isoschizomer of BspRI
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O recognition site is GGCC, enzyme does not cut Sulfolobus acidocaldarius DNA, as the recognition site in this DNA contains modified nucleotides Sulfolobus acidocaldarius ?
-
 ?
additional information the enzyme does not cut Sulfolobus acidocaldarius DNA, as the recognition sequence GGCC in this DNA contains modified nucleotides Sulfolobus acidocaldarius ?
-
 ?
pBR322DNA + H2O the tetranucleotide GGCC can be cleaved by SuaI either symmetrically or nonsymmetrically, thus producing termini with a single-stranded end Sulfolobus acidocaldarius pBR322 DNA fragments
-
 ?

Synonyms

Synonyms Comment Organism
SuaI
-
 Sulfolobus acidocaldarius
type II restriction endonuclease
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 at 40°C the activity is about 5times lower than at 60-70°C Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 30 min, 50% loss of activity Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Sulfolobus acidocaldarius