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Literature summary for 3.1.21.4 extracted from
- Isolation and characterization of type IIS restriction endonuclease from Neisseria cuniculi ATCC 14688 (2001), FEMS Microbiol. Lett., 196, 171-176.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | may substitute for Mg2+, as effective as Mg2+ | Moraxella cuniculi |  |
| Co2+ | may substitute for Mg2+, much less effective, relaxed specificity of enzyme | Moraxella cuniculi | |
| Mg2+ | required | Moraxella cuniculi | |
| Mn2+ | may substitute for Mg2+, much less effective, relaxed specificity of enzyme | Moraxella cuniculi | |
| additional information | Fe2+, Ni2+, Zn2+ may not substitute for Mg2+ | Moraxella cuniculi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
1 * 48000, SDS-PAGE, N-terminal amino acid sequence | Moraxella cuniculi |
| 51000 | - |
gel filtration | Moraxella cuniculi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moraxella cuniculi | - |
strain ATCC14688, enzyme NcuI, a homoisoschizomer of MboII | - |
| Moraxella cuniculi ATCC 14688 | - |
strain ATCC14688, enzyme NcuI, a homoisoschizomer of MboII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moraxella cuniculi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recognition site is 5-GAAGA-3, cleavage occurs 7 or 8 bp downstream and generates a single 3-protruding nucleotide | Moraxella cuniculi | ? | - |
? | |
| additional information | recognition site is 5-GAAGA-3, cleavage occurs 7 or 8 bp downstream and generates a single 3-protruding nucleotide | Moraxella cuniculi ATCC 14688 | ? | - |
? | |
| pNH20 + H2O | 84 bp SacI/HindII-fragment | Moraxella cuniculi | ? | - |
? | |
| pNH20 + H2O | 84 bp SacI/HindII-fragment | Moraxella cuniculi ATCC 14688 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, SDS-PAGE, N-terminal amino acid sequence | Moraxella cuniculi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Moraxella cuniculi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.9 | - |
Moraxella cuniculi |
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