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Information on EC 2.8.4.4 - [ribosomal protein S12] (aspartate89-C3)-methylthiotransferase

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IUBMB Comments
This bacterial enzyme binds two [4Fe-4S] clusters [2,3]. A bridge of five sulfur atoms is formed between the free Fe atoms of the two [4Fe-4S] clusters . In the first reaction the enzyme transfers a methyl group from AdoMet to the external sulfur ion of the sulfur bridge. In the second reaction the enzyme catalyses the reductive fragmentation of a second molecule of AdoMet, yielding a 5'-deoxyadenosine radical, which then attacks the methylated sulfur atom of the polysulfide bridge, resulting in the transfer of a methylsulfanyl group to aspartate89 [5,6]. The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes.
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Word Map
  • 2.8.4.4
  • transposition
  • transposable
  • piggybac
  • protein-dna
  • cut-and-paste
  • integrases
  • transpososome
  • metnase
  • subterminal
  • nonautonomous
  • beauty
  • transposase-dna
  • helix-turn-helix
  • transposase-mediated
  • cointegrate
  • tpase
  • setmar
  • transgenesis
  • p-element
  • himar1
  • hermes
  • mariner-like
  • hobo
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
L-aspartate89-[ribosomal protein S12]
+
sulfur-(sulfur carrier)
+
2
+
=
3-(methylsulfanyl)-L-aspartate89-[ribosomal protein S12]
+
+
(sulfur carrier)
+
+
+
oxidized acceptor
+
L-aspartate89-[ribosomal protein S12]
+
sulfur-(sulfur carrier)
=
+
L-aspartate89-[ribosomal protein S12]-methanethiol
+
(sulfur carrier)
L-aspartate89-[ribosomal protein S12]-methanethiol
+
+
=
3-(methylsulfanyl)-L-aspartate89-[ribosomal protein S12]
+
+
+
oxidized acceptor
Synonyms
ribosomal protein S12 methylthiotransferase, ribosome maturation factor RimO, RimO, S12 methylthiotransferase, yliG, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced acceptor = 3-(methylsulfanyl)-L-aspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized acceptor
show the reaction diagram
L-aspartate89-[ribosomal protein S12]-methanethiol + S-adenosyl-L-methionine + reduced acceptor = 3-(methylsulfanyl)-L-aspartate89-[ribosomal protein S12] + L-methionine + 5'-deoxyadenosine + oxidized acceptor
show the reaction diagram
S-adenosyl-L-methionine + L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) = S-adenosyl-L-homocysteine + L-aspartate89-[ribosomal protein S12]-methanethiol + (sulfur carrier)
show the reaction diagram
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