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Ligand sulfur-(sulfur carrier)

Basic Ligand Information

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (8 results)

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Show Natural Substrate (8 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.1.6

dethiobiotin + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin

738906

-

2.8.1.8

protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin

737720

-

2.8.1.8

protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster = protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster

737696

-

2.8.1.8

2 entries

2.8.4.3

N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

725354

-

2.8.4.4

Asp89-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthioaspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

724275

-

2.8.4.4

L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced acceptor = 3-methylthio-L-aspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized acceptor

737730

-

2.8.4.4

L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced acceptor = 3-methylthio-L-aspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized acceptor

738458

-

2.8.4.4

3 entries

2.8.4.5

N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

725403

-

Substrate in Enzyme-catalyzed Reactions (20 results)

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Show Substrate (20 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.1.6

dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin

762502

-

2.8.1.6

dethiobiotin + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin

738906

-

2.8.1.8

biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin = biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin

737696

-

2.8.1.8

Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite = Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite

737696

-

2.8.1.8

H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] dithionite = H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] dithionite

737720

-

2.8.1.8

H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] flavodoxin = H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] flavodoxin

737720

-

2.8.1.8

protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin

737720

-

2.8.1.8

protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster = protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster

737696

-

2.8.1.8

[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite = [8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite

737696

-

2.8.4.3

N6-dimethylallyladenine in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-dimethylallyladenine in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

727847

-

2.8.4.3

N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

725354, 727689, 727847, 728356

-

2.8.4.3

N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine = 2-thio-N6-dimethylallyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

727847

-

2.8.4.4

3-methylthioaspartate89-[ribosomal protein S12 20mer peptide] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3,3-bismethylthioaspartate89-[ribosomal protein S12 20mer peptide] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

727871

-

2.8.4.4

Asp88-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthioaspartate88-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

728300, 728655

-

2.8.4.4

Asp89-[ribosomal protein S12 20mer peptide] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthioaspartate89-[ribosomal protein S12 20mer peptide] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

727871

-

2.8.4.4

Asp89-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthioaspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

724275

-

2.8.4.4

L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced acceptor = 3-methylthio-L-aspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized acceptor

737730

-

2.8.4.4

L-aspartate89-[ribosomal protein S12] + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced acceptor = 3-methylthio-L-aspartate89-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized acceptor

738458

-

2.8.4.4

N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

727689, 728356

-

2.8.1.6

2 entries

2.8.1.8

7 entries

2.8.4.3

3 entries

2.8.4.4

7 entries

2.8.4.5

N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine

725403

-

Enzyme Kinetic Parameters

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

724275

19736993

Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily

2009

Lee, K.H.; Saleh, L.; Anton, B.P.; Madinger, C.L.; Benner, J.S.; Iwig, D.F.; Roberts, R.J.; Krebs, C.; Booker, S.J.

Biochemistry

48

10162-10174

725354

12766153

MiaB protein from Thermotoga maritima. Characterization of an extremely thermophilic tRNA-methylthiotransferase

2003

Pierrel, F.; Hernandez, H.L.; Johnson, M.K.; Fontecave, M.; Atta, M.

J. Biol. Chem.

278

29515-29524

725403

20584901

Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA

2010

Arragain, S.; Handelman, S.K.; Forouhar, F.; Wei, F.Y.; Tomizawa, K.; Hunt, J.F.; Douki, T.; Fontecave, M.; Mulliez, E.; Atta, M.

J. Biol. Chem.

285

28425-28433

727689

23991893

Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB

2013

Landgraf, B.J.; Arcinas, A.J.; Lee, K.H.; Booker, S.J.

J. Am. Chem. Soc.

135

15404-15416

727847

15339930

MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA

2004

Pierrel, F.; Douki, T.; Fontecave, M.; Atta, M.

J. Biol. Chem.

279

47555-47563

727871

20007320

Post-translational modification of ribosomal proteins: structural and functional characterization of RimO from Thermotoga maritima, a radical S-adenosylmethionine methylthiotransferase

2010

Arragain, S.; Garcia-Serres, R.; Blondin, G.; Douki, T.; Clemancey, M.; Latour, J.M.; Forouhar, F.; Neely, H.; Montelione, G.T.; Hunt, J.F.; Mulliez, E.; Fontecave, M.; Atta, M.

J. Biol. Chem.

285

5792-5801

728300

21169565

A proteomic and transcriptomic approach reveals new insight into beta-methylthiolation of Escherichia coli ribosomal protein

2011

Strader, M.; Costantino, N.; Elkins, C.; Chen, C.; Patel, I.; Makusky, A.; Choy, J.; Court, D.; Markey, S.; Kowalak, J.

Mol. Cell. Proteomics

10

M110.005199

728356

23542644

Two Fe-S clusters catalyze sulfur insertion by radical-SAM methylthiotransferases

2013

Forouhar, F.; Arragain, S.; Atta, M.; Gambarelli, S.; Mouesca, J.M.; Hussain, M.; Xiao, R.; Kieffer-Jaquinod, S.; Seetharaman, J.; Acton, T.B.; Montelione, G.T.; Mulliez, E.; Hunt, J.F.; Fontecave, M.

Nat. Chem. Biol.

9

333-338

728655

18252828

RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli

2008

Anton, B.P.; Saleh, L.; Benner, J.S.; Raleigh, E.A.; Kasif, S.; Roberts, R.J.

Proc. Natl. Acad. Sci. USA

105

1826-1831

737696

24901788

Evidence for a catalytically and kinetically competent enzyme-substrate cross-linked intermediate in catalysis by lipoyl synthase

2014

Lanz, N.D.; Pandelia, M.E.; Kakar, E.S.; Lee, K.H.; Krebs, C.; Booker, S.J.

Biochemistry

53

4557-4572

737720

26841001

Characterization of Lipoyl Synthase from Mycobacterium tuberculosis

2016

Lanz, N.D.; Lee, K.H.; Horstmann, A.K.; Pandelia, M.E.; Cicchillo, R.M.; Krebs, C.; Booker, S.J.

Biochemistry

55

1372-1383

737730

27677419

Redox behavior of the S-adenosylmethionine (SAM)-binding Fe-S cluster in methylthiotransferase RimO, toward understanding dual SAM activity

2016

Molle, T.; Moreau, Y.; Clemancey, M.; Forouhar, F.; Ravanat, J.L.; Duraffourg, N.; Fourmond, V.; Latour, J.M.; Gambarelli, S.; Mulliez, E.; Atta, M.

Biochemistry

55

5798-5808

738458

26871608

Stereochemical course of the reaction catalyzed by RimO, a radical SAM methylthiotransferase

2016

Landgraf, B.J.; Booker, S.J.

J. Am. Chem. Soc.

138

2889-2892

738906

27117847

The effects of light and temperature on biotin synthesis in pea sprouts

2016

Kamiyama, S.; Ohnuki, R.; Moriki, A.; Abe, M.; Ishiguro, M.; Sone, H.

J. Nutr. Sci. Vitaminol.

62

19-25

762502

27156617

Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis

2016

Magwamba, C.C.; Rukseree, K.; Palittapongarnpim, P.

Tuberculosis

98

42-49

762502

27156617

Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis

2016

Magwamba, C.C.; Rukseree, K.; Palittapongarnpim, P.

Tuberculosis

98

42-49

762502

2 entries

Links to other databases for sulfur-(sulfur carrier)

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Show Links (1 entry)