Literature summary for 2.8.4.4 extracted from

Strader, M.; Costantino, N.; Elkins, C.; Chen, C.; Patel, I.; Makusky, A.; Choy, J.; Court, D.; Markey, S.; Kowalak, J.
A proteomic and transcriptomic approach reveals new insight into beta-methylthiolation of Escherichia coli ribosomal protein (2011), Mol. Cell. Proteomics, 10, M110.005199.

Search for more literature for 2.8.4.4

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AEI4	-	-
Escherichia coli W3110 / ATCC 27325	P0AEI4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Asp88-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine	substrate Escherichia coli S12 is modified at residue Asp88	Escherichia coli	3-methylthioaspartate88-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine	-	?
Asp88-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine	substrate Escherichia coli S12 is modified at residue Asp88	Escherichia coli W3110 / ATCC 27325	3-methylthioaspartate88-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine	-	?

General Information

General Information	Comment	Organism
physiological function	proteins RimO and YcaO, a conserved protein of unknown function, dramatically impact the modification status of ribosomal protein S12. The YcaO protein is involved in beta-methylthiolation of S12 and its absence impairs the ability of RimO to modify S12. The Escherichia coli specific beta-methylthiolation likely occurs when S12 is assembled as part of a ribosomal subunit. A RimO knockout mutant under aerobic conditions exhibits a 2fold decrease in transcription levels for a subset of genes belonging to operons that require the fumarate and nitrate reductase regulator for transcriptional activation and at least one of the nitrate and nitrite transcriptional response regulators NarL and NarP	Escherichia coli

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Release 2023.1 (January 2023)