Literature summary for 2.8.4.4 extracted from

Landgraf, B.J.; Arcinas, A.J.; Lee, K.H.; Booker, S.J.
Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB (2013), J. Am. Chem. Soc., 135, 15404-15416.

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Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X2H6	-	-
Thermotoga maritima DSM 3109	Q9X2H6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme catalyzes methyl transfer from 2 S-adenosyl-L-methionine to an acid/base labile acceptor on the protein in the absence of their respective macromolecular substrates. Denaturation of the 2 S-adenosyl-L-methionine-treated protein with acid results in production of ethanethiol. When the enzyme is first incubated with 2 S-adenosyl-L-methionine in the absence of substrate and reductant and then incubated with excess S-adenosyl-L-[methyl-d3]methionine in the presence of substrate and reductant, production of the unlabeled product precedes production of the deuterated product, showing that the methylated species is chemically and kinetically competent to be an intermediate	Thermotoga maritima	?	-	?
additional information	enzyme catalyzes methyl transfer from 2 S-adenosyl-L-methionine to an acid/base labile acceptor on the protein in the absence of their respective macromolecular substrates. Denaturation of the 2 S-adenosyl-L-methionine-treated protein with acid results in production of ethanethiol. When the enzyme is first incubated with 2 S-adenosyl-L-methionine in the absence of substrate and reductant and then incubated with excess S-adenosyl-L-[methyl-d3]methionine in the presence of substrate and reductant, production of the unlabeled product precedes production of the deuterated product, showing that the methylated species is chemically and kinetically competent to be an intermediate	Thermotoga maritima DSM 3109	?	-	?
N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine	-	Thermotoga maritima	2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine	-	?
N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine	-	Thermotoga maritima DSM 3109	2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine	-	?

Synonyms

Synonyms	Comment	Organism
ribosomal protein S12 methylthiotransferase	-	Thermotoga maritima
ribosome maturation factor RimO	-	Thermotoga maritima
RimO	-	Thermotoga maritima
S12 methylthiotransferase	-	Thermotoga maritima

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Release 2023.1 (January 2023)