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3-mercaptopyruvate + activated tRNA
? + S-tRNA
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
L-cysteine + Bacillus subtilis 'activated' tRNA
L-serine + tRNA containing a thionucleotide
L-cysteine + Bos taurus 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
calf liver tRNA better substrate than rat liver tRNA
-
-
r
L-cysteine + denatured DNA
L-serine + ?
-
DNA prepared from various sources appears to function as a good sulfur acceptor
-
-
?
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
L-cysteine + poly A-2poly U
L-serine + S-tRNA
L-cysteine + poly A-poly U
L-serine + S-tRNA
L-cysteine + poly G,U
L-serine + S-tRNA
-
G:U = 2
-
-
?
L-cysteine + rabbit liver 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Rattus norvegicus brain 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
better substrate than liver tRNA
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
L-serine + tRNA containing a thionucleotide
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
L-serine + tRNA containing a thionucleotide
sulfide + tRNA-uridine
tRNA-4-thiouridine
the enzyme is invoved in biosynthesis of 4-thiouridine in tRNA. 4-Thiouridine, is a modified nucleotide of tRNA that is conserved from bacteria to archaea, where it functions as a sensor for near-UV irradiation
-
-
?
sulfide + [tRNACys]-uridine
[tRNACys]-4-thiouridine
proposed mechanism: first, a sulfur donor (equivalent of S2-) attacks the Cys265Cys268 disulfide linkage to generate a persulfide on either Cys265 or Cys268, leaving the other Cys as a free thiol. Then, a thiolate derived from deprotonation of the free thiol attacks the bridging sulfur of the persulfide to liberate the terminal sulfur. After donation of the sulfur (in the -2 oxidation state) to form 4-thiouridine, the disulfide (with both sulfurs in the -1 oxidation state) is consequently regenerated. No exogenous electron donor or acceptor is required for the catalytic cycle
-
-
?
additional information
?
-
3-mercaptopyruvate + activated tRNA
? + S-tRNA
-
-
-
-
?
3-mercaptopyruvate + activated tRNA
? + S-tRNA
-
in vitro
-
-
?
3-mercaptopyruvate + activated tRNA
? + S-tRNA
-
reaction product is sensitive to deacylation
-
-
?
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Bacillus subtilis 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Bacillus subtilis 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
-
?
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
-
?
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA
-
-
r
L-cysteine + poly A-2poly U
L-serine + S-tRNA
-
-
-
-
?
L-cysteine + poly A-2poly U
L-serine + S-tRNA
-
-
-
-
?
L-cysteine + poly A-poly U
L-serine + S-tRNA
-
-
-
-
?
L-cysteine + poly A-poly U
L-serine + S-tRNA
-
-
-
-
?
L-cysteine + Rattus norvegicus liver 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
poorest substrate
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
same activity with baker's yeast tRNA as RNA acceptor
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
-
r
L-cysteine + tRNA
?
-
-
-
-
?
L-cysteine + tRNA
?
-
-
-
-
?
additional information
?
-
-
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
-
-
?
additional information
?
-
-
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
-
-
?
additional information
?
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
additional information
?
-
-
2-thiocytidine is the major thionucleotide formed in vitro, 4-thiouridine is the major product in vivo
-
-
?
additional information
?
-
-
2-thiocytidine is the major thionucleotide formed in vitro, 4-thiouridine is the major product in vivo
-
-
?
additional information
?
-
-
catalyzes the transfer of sulfur to various tRNSs from microbial sources, tRNA of higher organisms, rRNA, native and denatured DNA does not accept sulfur
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
associated in a complex with tRNA mehtyltransferase and aminoacyl-tRNA synthetase
-
-
?
additional information
?
-
-
rRNA, mRNAand synthetic ribohomopolymers are no acceptor substrates, little or no reaction with E. coli B rRNA, phage MS2 RNA, polyU, polyC, polyA, and polyG
-
-
?
additional information
?
-
-
only tRNAs are effective sulfur acceptors, little or no reaction with rat liver rRNA, salmon sperm DNA, polyuridylic acid and polyadenylic acid
-
-
?
additional information
?
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
additional information
?
-
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
sulfide + tRNA-uridine
tRNA-4-thiouridine
the enzyme is invoved in biosynthesis of 4-thiouridine in tRNA. 4-Thiouridine, is a modified nucleotide of tRNA that is conserved from bacteria to archaea, where it functions as a sensor for near-UV irradiation
-
-
?
additional information
?
-
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
sulfur deficient tRNA is the natural substrate
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + 'activated' tRNA
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
?
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
additional information
?
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
-
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs
-
-
?
additional information
?
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
additional information
?
-
-
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Wong, T.W.; Weiss, S.B.; Eliceiri, G.L.; Bryant, J.
Ribonucleic acid sulfurtransferase from Bacillus subtilis W168. Sulfuration with beta-mercaptopyruvate and properties of the enzyme system
Biochemistry
9
2376-2386
1970
Bacillus subtilis, Bacillus subtilis W168
brenda
Abrell, J.W.; Kaufman, E.E.; Lipsett, M.N.
The biosynthesis of 4-thiouridylate. Separation and purification of two enzymes in the transfer ribonucleic acid-sulfurtransferase system
J. Biol. Chem.
246
294-301
1971
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Harris, C.L.; Titchener, E.B.
Sulfur-deficient transfer ribonucleic acid. The natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli
Biochemistry
10
4207-4212
1971
Escherichia coli, Escherichia coli HfrC
brenda
Wong, T.W.; Harris, M.A.; Jankowicz, C.A.
Transfer ribonucleic acid sulfurtransferase isolated from rat cerebral hemispheres
Biochemistry
13
2805-2812
1974
Rattus norvegicus
brenda
Thimmappaya, B.; Cherayil, J.D.
Studies on transfer ribonucleic acid sulfur transferase from Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
Pseudomonas aeruginosa
-
brenda
Wong, T.W.; Harris, M.A.; Morris, H.P.
The presence of an inhibitor of RNA sulfurtransferase in Morris hepatomas
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
Rattus norvegicus
brenda
Harris, C.L.; Marin, K.; Stewart, D.
tRNA sulfurtransferase: a member of the aminoacyl-tRNA synthetase complex in rat liver
Biochem. Biophys. Res. Commun.
79
657-662
1977
Rattus norvegicus
brenda
Harris, C.L.
Mammalian tRNA sulfurtransferase: properties of the enzyme in rat liver
Nucleic Acids Res.
5
599-613
1978
Rattus norvegicus
brenda
Numata, T.; Ikeuchi, Y.; Fukai, S.; Suzuki, T.; Nureki, O.
Snapshots of tRNA sulphuration via an adenylated intermediate
Nature
442
419-424
2006
Escherichia coli (P25745)
brenda
Dewez, M.; Bauer, F.; Dieu, M.; Raes, M.; Vandenhaute, J.; Hermand, D.
The conserved wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
Caenorhabditis elegans (O76365), Schizosaccharomyces pombe (O94282), Schizosaccharomyces pombe
brenda
Liu, Y.; Zhu, X.; Nakamura, A.; Orlando, R.; Soell, D.; Whitman, W.B.
Biosynthesis of 4-thiouridine in tRNA in the methanogenic archaeon Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
Methanococcus maripaludis (Q6LXJ6), Methanococcus maripaludis
brenda