BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

Ribonucleic acid sulfurtransferase from Bacillus subtilis W168. Sulfuration with beta-mercaptopyruvate and properties of the enzyme system

Wong, T.W.; Weiss, S.B.; Eliceiri, G.L.; Bryant, J.; Biochemistry 9, 2376-2386 (1970)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Mercaptopyruvate
competitive inhibitor, supresses the transfer of sulfur to tRNA
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0069
-
3-Mercaptopyruvate
pH 7.8, 37°C
Bacillus subtilis
1
-
L-cysteine
pH 7.8, 37°C
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Bacillus subtilis
-
L-serine + tRNA containing a thionucleotide
-
Bacillus subtilis
r
L-cysteine + 'activated' tRNA
Bacillus subtilis W168
-
L-serine + tRNA containing a thionucleotide
-
Bacillus subtilis W168
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
W168
-
Bacillus subtilis W168
-
W168
-
Purification (Commentary)
Commentary
Organism
partially
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00815
-
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
-
643805
Bacillus subtilis
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
643805
Bacillus subtilis
r
L-cysteine + 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
643805
Bacillus subtilis W168
r
L-cysteine + Bacillus subtilis 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Bacillus subtilis 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + denatured DNA
DNA prepared from various sources appears to function as a good sulfur acceptor
643805
Bacillus subtilis
L-serine + ?
-
-
-
?
L-cysteine + E. coli 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
643805
Bacillus subtilis
?
-
-
-
-
additional information
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
643805
Bacillus subtilis W168
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
absolute requirement, other nucleotides are inactive
Bacillus subtilis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.005
-
3-Mercaptopyruvate
pH 7.8, 37°C
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
absolute requirement, other nucleotides are inactive
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Mercaptopyruvate
competitive inhibitor, supresses the transfer of sulfur to tRNA
Bacillus subtilis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.005
-
3-Mercaptopyruvate
pH 7.8, 37°C
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0069
-
3-Mercaptopyruvate
pH 7.8, 37°C
Bacillus subtilis
1
-
L-cysteine
pH 7.8, 37°C
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Bacillus subtilis
-
L-serine + tRNA containing a thionucleotide
-
Bacillus subtilis
r
L-cysteine + 'activated' tRNA
Bacillus subtilis W168
-
L-serine + tRNA containing a thionucleotide
-
Bacillus subtilis W168
r
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00815
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
-
643805
Bacillus subtilis
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
643805
Bacillus subtilis
r
L-cysteine + 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
643805
Bacillus subtilis W168
r
L-cysteine + Bacillus subtilis 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Bacillus subtilis 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + denatured DNA
DNA prepared from various sources appears to function as a good sulfur acceptor
643805
Bacillus subtilis
L-serine + ?
-
-
-
?
L-cysteine + E. coli 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643805
Bacillus subtilis
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643805
Bacillus subtilis W168
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
643805
Bacillus subtilis
?
-
-
-
-
additional information
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU
643805
Bacillus subtilis W168
?
-
-
-
-
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
-
-
1
-
4
-
-
1
-
1
-
1
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
-
-
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
-
-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
1
1
-
1
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
-
5
1
-
1
-
2
-
177
-
-
1
-
-
-
-
2
12
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
1
-
1
-
2
-
-
-
1
-
-
-
2
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
-
-
-
-
-
-
-
2
-
1
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
-
-
-
-
-
-
1
2
-
1
-
2
-
4
-
-
1
-
-
-
1
-
13
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
1
1
2
-
1
-
2
-
-
-
1
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-