BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

tRNA sulfurtransferase: a member of the aminoacyl-tRNA synthetase complex in rat liver

Harris, C.L.; Marin, K.; Stewart, D.; Biochem. Biophys. Res. Commun. 79, 657-662 (1977)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000372
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, freezing causes a 50% loss of sulfurtransferase activity
Rattus norvegicus
0-4°C, 50% glycerol, storage of the complex for 1 week results in a loss of 30-60% of the 3 different activities
Rattus norvegicus
0°C, only slight loss of enzymatic activity on overnight storage
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + 'activated' tRNA
-
643811
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643811
Rattus norvegicus
r
additional information
associated in a complex with tRNA mehtyltransferase and aminoacyl-tRNA synthetase
643811
Rattus norvegicus
?
-
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000372
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, freezing causes a 50% loss of sulfurtransferase activity
Rattus norvegicus
0-4°C, 50% glycerol, storage of the complex for 1 week results in a loss of 30-60% of the 3 different activities
Rattus norvegicus
0°C, only slight loss of enzymatic activity on overnight storage
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + 'activated' tRNA
-
643811
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643811
Rattus norvegicus
r
additional information
associated in a complex with tRNA mehtyltransferase and aminoacyl-tRNA synthetase
643811
Rattus norvegicus
?
-
-
-
-
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
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1
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4
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1
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1
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1
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6
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1
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2
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1
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1
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1
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1
1
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4
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1
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1
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1
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-
1
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-
-
-
2
-
1
-
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1
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1
1
-
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-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
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2
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4
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4
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4
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2
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4
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4
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676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
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-
1
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1
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1
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1
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643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
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-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
1
-
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-
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-
1
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-
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-
1
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-
-
1
-
1
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-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
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-
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-
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1
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1
1
3
2
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1
1
3
2
-
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643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
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1
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1
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1
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1
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2
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1
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2
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2
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1
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1
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1
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2
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-
1
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643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
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-
-
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1
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1
1
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1
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1
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2
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1
2
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1
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1
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1
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1
1
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1
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2
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1
2
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-
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643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
-
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1
-
1
1
-
1
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
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1
1
-
1
-
-
-
1
-
1
1
1
7
-
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-
1
1
-
-
-
-
-
-
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-
643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
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5
1
-
1
-
2
-
177
-
-
1
-
-
-
-
2
12
-
-
-
-
-
1
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-
2
-
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-
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-
-
2
-
-
-
-
5
-
1
-
1
-
2
-
-
-
1
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-
-
2
12
-
-
-
-
-
1
-
-
-
-
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-
-
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643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
-
-
-
-
-
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2
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1
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2
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2
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8
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1
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1
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2
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1
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2
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8
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643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
-
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-
-
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1
2
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1
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2
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4
-
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1
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1
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13
-
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1
1
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1
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1
1
2
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1
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2
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1
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1
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13
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