Literature summary for 2.8.1.4 extracted from

Numata, T.; Ikeuchi, Y.; Fukai, S.; Suzuki, T.; Nureki, O.
Snapshots of tRNA sulphuration via an adenylated intermediate (2006), Nature, 442, 419-424.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with tRNAGlu	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25745	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster	on enzyme activation, an alpha-helix overhanging the active site is restructured into an idiosyncratic beta-hairpin-containing loop, which packs the flipped-out U34 deeply into the catalytic pocket and triggers the activation of the catalytic cysteine residues. The adenylated RNA intermediate is trapped. The active closed-conformation of the complex ensures accurate sulfur incorporation into the activated uridine carbon by forming a catalytic chamber to prevent solvent from accessing the catalytic site	Escherichia coli	a

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Release 2023.1 (January 2023)