BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

Snapshots of tRNA sulphuration via an adenylated intermediate

Numata, T.; Ikeuchi, Y.; Fukai, S.; Suzuki, T.; Nureki, O.; Nature 442, 419-424 (2006)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
in complex with tRNAGlu
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P25745
-
-
Reaction
Reaction
Commentary
Organism
ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
on enzyme activation, an alpha-helix overhanging the active site is restructured into an idiosyncratic beta-hairpin-containing loop, which packs the flipped-out U34 deeply into the catalytic pocket and triggers the activation of the catalytic cysteine residues. The adenylated RNA intermediate is trapped. The active closed-conformation of the complex ensures accurate sulfur incorporation into the activated uridine carbon by forming a catalytic chamber to prevent solvent from accessing the catalytic site
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with tRNAGlu
Escherichia coli
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
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1
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4
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1
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1
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1
-
6
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1
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-
-
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2
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1
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1
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1
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1
1
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4
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1
-
1
-
1
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-
1
-
-
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-
2
-
1
-
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1
-
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1
1
-
-
-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
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2
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4
-
4
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4
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2
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4
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4
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676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
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1
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1
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1
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643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
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-
-
-
-
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1
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1
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1
-
2
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-
-
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-
9
-
1
7
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-
1
-
-
1
1
1
1
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-
1
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1
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1
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1
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9
-
1
7
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1
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1
1
1
-
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-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
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1
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1
1
3
2
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1
1
3
2
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643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
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1
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1
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1
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1
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2
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1
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643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
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-
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1
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1
1
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1
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1
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2
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1
2
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1
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1
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1
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1
1
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1
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2
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1
2
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-
643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
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1
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1
1
-
1
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1
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1
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1
1
1
7
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1
1
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2
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1
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2
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1
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1
1
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1
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1
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1
1
1
7
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1
1
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643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
-
5
1
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1
-
2
-
177
-
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1
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2
12
-
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1
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2
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2
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5
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1
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1
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2
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1
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2
12
-
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1
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643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
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2
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1
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2
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2
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8
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1
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1
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2
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8
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643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
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1
2
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1
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2
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4
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1
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1
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13
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1
1
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1
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1
1
2
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1
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2
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1
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1
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13
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