Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | requires a thiol for optimal sulfur transfer | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + 'activated' tRNA | Rattus norvegicus | - |
L-serine + tRNA containing a thionucleotide | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
buffalo | - |
Purification (Comment) | Organism |
---|---|
partially | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | cerebral hemisphere | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000658 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-75°C, ammonium sulfate fractionated preparation retains its activity for several months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + activated tRNA | in vitro | Rattus norvegicus | ? + S-tRNA | - |
? | |
L-cysteine + 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + E. coli 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + rabbit liver 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + Rattus norvegicus liver 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
additional information | rRNA, mRNAand synthetic ribohomopolymers are no acceptor substrates, little or no reaction with E. coli B rRNA, phage MS2 RNA, polyU, polyC, polyA, and polyG | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus | |
additional information | sulfur transfer obtained with ADP is apparently due to the formation of ATP by the reaction 2 ADP = ATP + AMP, AMP is totally inactive as activating nucleotide | Rattus norvegicus |