BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

Transfer ribonucleic acid sulfurtransferase isolated from rat cerebral hemispheres

Wong, T.W.; Harris, M.A.; Jankowicz, C.A.; Biochemistry 13, 2805-2812 (1974)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
requires a thiol for optimal sulfur transfer
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Rattus norvegicus
-
L-serine + tRNA containing a thionucleotide
-
Rattus norvegicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
buffalo
-
Purification (Commentary)
Commentary
Organism
partially
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
cerebral hemisphere
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000658
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-75°C, ammonium sulfate fractionated preparation retains its activity for several months
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
in vitro
643808
Rattus norvegicus
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643808
Rattus norvegicus
r
L-cysteine + E. coli 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + rabbit liver 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
rRNA, mRNAand synthetic ribohomopolymers are no acceptor substrates, little or no reaction with E. coli B rRNA, phage MS2 RNA, polyU, polyC, polyA, and polyG
643808
Rattus norvegicus
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Rattus norvegicus
additional information
sulfur transfer obtained with ADP is apparently due to the formation of ATP by the reaction 2 ADP = ATP + AMP, AMP is totally inactive as activating nucleotide
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
requires a thiol for optimal sulfur transfer
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Rattus norvegicus
additional information
sulfur transfer obtained with ADP is apparently due to the formation of ATP by the reaction 2 ADP = ATP + AMP, AMP is totally inactive as activating nucleotide
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Rattus norvegicus
-
L-serine + tRNA containing a thionucleotide
-
Rattus norvegicus
r
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
cerebral hemisphere
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000658
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-75°C, ammonium sulfate fractionated preparation retains its activity for several months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
in vitro
643808
Rattus norvegicus
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643808
Rattus norvegicus
r
L-cysteine + E. coli 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + rabbit liver 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
643808
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
rRNA, mRNAand synthetic ribohomopolymers are no acceptor substrates, little or no reaction with E. coli B rRNA, phage MS2 RNA, polyU, polyC, polyA, and polyG
643808
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
-
Rattus norvegicus
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
-
-
1
-
4
-
-
1
-
1
-
1
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
-
-
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
-
-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
1
1
-
1
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
-
5
1
-
1
-
2
-
177
-
-
1
-
-
-
-
2
12
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
1
-
1
-
2
-
-
-
1
-
-
-
2
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
-
-
-
-
-
-
-
2
-
1
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
-
-
-
-
-
-
1
2
-
1
-
2
-
4
-
-
1
-
-
-
1
-
13
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
1
1
2
-
1
-
2
-
-
-
1
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-