BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

The biosynthesis of 4-thiouridylate. Separation and purification of two enzymes in the transfer ribonucleic acid-sulfurtransferase system

Abrell, J.W.; Kaufman, E.E.; Lipsett, M.N.; J. Biol. Chem. 246, 294-301 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
inhibits the synthesis of s4U
Escherichia coli
Co2+
inhibits the synthesis of s4U
Escherichia coli
Fe2+
inhibits the synthesis of s4U
Escherichia coli
Ni2+
inhibits the synthesis of s4U
Escherichia coli
Zn2+
inhibits the synthesis of s4U
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
0.0016
L-cysteine
pH 7.8, 37C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Escherichia coli
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli
r
L-cysteine + 'activated' tRNA
Escherichia coli B / ATCC 11303
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli B / ATCC 11303
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
Commentary
Organism
sulfurtransferase system with 2 enzymes
Escherichia coli
Storage Stability
Storage Stability
Organism
-20C, partly purified pooled protein fractions can be stored indefinitely for periods up to several months
Escherichia coli
4C, addition of toluene, can be stored indefinitely for periods up to several months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + 'activated' tRNA
-
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli
r
L-cysteine + 'activated' tRNA
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli
r
L-cysteine + 'activated' tRNA
-
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli B / ATCC 11303
r
L-cysteine + 'activated' tRNA
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli B / ATCC 11303
r
L-cysteine + E. coli 'activated' tRNA
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + poly A-2poly U
-
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-2poly U
-
643806
Escherichia coli B / ATCC 11303
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-poly U
-
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-poly U
-
643806
Escherichia coli B / ATCC 11303
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly G,U
G:U = 2
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
same activity with baker's yeast tRNA as RNA acceptor
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.4
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli
additional information
beta-mercaptopyruvate is inactive either as a cofactor or as a sulfur donor
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli
additional information
beta-mercaptopyruvate is inactive either as a cofactor or as a sulfur donor
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
inhibits the synthesis of s4U
Escherichia coli
Co2+
inhibits the synthesis of s4U
Escherichia coli
Fe2+
inhibits the synthesis of s4U
Escherichia coli
Ni2+
inhibits the synthesis of s4U
Escherichia coli
Zn2+
inhibits the synthesis of s4U
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
0.0016
L-cysteine
pH 7.8, 37C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Escherichia coli
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli
r
L-cysteine + 'activated' tRNA
Escherichia coli B / ATCC 11303
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
L-serine + tRNA containing a thionucleotide
-
Escherichia coli B / ATCC 11303
r
Purification (Commentary) (protein specific)
Commentary
Organism
sulfurtransferase system with 2 enzymes
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20C, partly purified pooled protein fractions can be stored indefinitely for periods up to several months
Escherichia coli
4C, addition of toluene, can be stored indefinitely for periods up to several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + 'activated' tRNA
-
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli
r
L-cysteine + 'activated' tRNA
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli
r
L-cysteine + 'activated' tRNA
-
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli B / ATCC 11303
r
L-cysteine + 'activated' tRNA
enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
643806
Escherichia coli B / ATCC 11303
r
L-cysteine + E. coli 'activated' tRNA
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
Escherichia coli B tRNA and irradiated Escherichia coli tRNA, S-poor Escherichia coli tRNA also acts as RNA acceptors
643806
Escherichia coli B / ATCC 11303
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + poly A-2poly U
-
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-2poly U
-
643806
Escherichia coli B / ATCC 11303
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-poly U
-
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly A-poly U
-
643806
Escherichia coli B / ATCC 11303
L-serine + S-tRNA
-
-
-
?
L-cysteine + poly G,U
G:U = 2
643806
Escherichia coli
L-serine + S-tRNA
-
-
-
?
L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
same activity with baker's yeast tRNA as RNA acceptor
643806
Escherichia coli
L-serine + tRNA containing a thionucleotide
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.4
-
Escherichia coli
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
-
-
1
-
4
-
-
1
-
1
-
1
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
-
-
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
-
-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
1
1
-
1
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
-
5
1
-
1
-
2
-
177
-
-
1
-
-
-
-
2
12
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
1
-
1
-
2
-
-
-
1
-
-
-
2
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
-
-
-
-
-
-
-
2
-
1
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
-
-
-
-
-
-
1
2
-
1
-
2
-
4
-
-
1
-
-
-
1
-
13
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
1
1
2
-
1
-
2
-
-
-
1
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-