BRENDA - Enzyme Database show
show all sequences of 2.8.1.4

Mammalian tRNA sulfurtransferase: properties of the enzyme in rat liver

Harris, C.L.; Nucleic Acids Res. 5, 599-613 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cysteine
inhibits reaction with 3-mercaptopyruvate
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Rattus norvegicus
-
L-serine + tRNA containing a thionucleotide
-
Rattus norvegicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Wistar albino
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Rattus norvegicus
-
brain
cerebral cortex, lowest activity
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
muscle
highest activity
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
-60°C, instable on storage, overnight storage results in 50% lost of activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
reaction product is sensitive to deacylation
643812
Rattus norvegicus
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643812
Rattus norvegicus
r
L-cysteine + Bos taurus 'activated' tRNA
calf liver tRNA better substrate than rat liver tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
sulfur deficient tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus brain 'activated' tRNA
better substrate than liver tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
poorest substrate
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
only tRNAs are effective sulfur acceptors, little or no reaction with rat liver rRNA, salmon sperm DNA, polyuridylic acid and polyadenylic acid
643812
Rattus norvegicus
?
-
-
-
-
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
37
at 10°C rate of sulfur transfer being 1/3 that at 37°C
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
about 75% of activity maximum at pH 7.0, about 55% of activity maximum at pH 9.0
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
inactivated
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cysteine
inhibits reaction with 3-mercaptopyruvate
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-cysteine + 'activated' tRNA
Rattus norvegicus
-
L-serine + tRNA containing a thionucleotide
-
Rattus norvegicus
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Rattus norvegicus
-
brain
cerebral cortex, lowest activity
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
muscle
highest activity
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
-60°C, instable on storage, overnight storage results in 50% lost of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-mercaptopyruvate + activated tRNA
reaction product is sensitive to deacylation
643812
Rattus norvegicus
? + S-tRNA
-
-
-
?
L-cysteine + 'activated' tRNA
-
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
643812
Rattus norvegicus
r
L-cysteine + Bos taurus 'activated' tRNA
calf liver tRNA better substrate than rat liver tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + E. coli 'activated' tRNA
sulfur deficient tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus brain 'activated' tRNA
better substrate than liver tRNA
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
L-cysteine + Rattus norvegicus liver 'activated' tRNA
poorest substrate
643812
Rattus norvegicus
L-serine + tRNA containing a thionucleotide
-
-
-
r
additional information
only tRNAs are effective sulfur acceptors, little or no reaction with rat liver rRNA, salmon sperm DNA, polyuridylic acid and polyadenylic acid
643812
Rattus norvegicus
?
-
-
-
-
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
37
at 10°C rate of sulfur transfer being 1/3 that at 37°C
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
about 75% of activity maximum at pH 7.0, about 55% of activity maximum at pH 9.0
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
inactivated
Rattus norvegicus
Other publictions for EC 2.8.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725494
Liu
Biosynthesis of 4-thiouridine ...
Methanococcus maripaludis
J. Biol. Chem.
287
36683-36692
2012
-
-
1
-
4
-
-
1
-
1
-
1
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
694891
Dewez
The conserved wobble uridine t ...
Caenorhabditis elegans, Schizosaccharomyces pombe
Proc. Natl. Acad. Sci. USA
105
5459-5464
2008
-
-
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676149
Numata
Snapshots of tRNA sulphuration ...
Escherichia coli
Nature
442
419-424
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643812
Harris
Mammalian tRNA sulfurtransfera ...
Rattus norvegicus
Nucleic Acids Res.
5
599-613
1978
-
-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
9
-
1
7
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
643811
Harris
tRNA sulfurtransferase: a memb ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
79
657-662
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643809
Thimmappaya
-
Studies on transfer ribonuclei ...
Pseudomonas aeruginosa
Indian J. Biochem. Biophys.
12
405-407
1975
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643810
Wong
The presence of an inhibitor o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
65
1137-1145
1975
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643808
Wong
Transfer ribonucleic acid sulf ...
Rattus norvegicus
Biochemistry
13
2805-2812
1974
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
1
1
-
1
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643806
Abrell
The biosynthesis of 4-thiourid ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
246
294-301
1971
-
-
-
-
-
-
5
1
-
1
-
2
-
177
-
-
1
-
-
-
-
2
12
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
1
-
1
-
2
-
-
-
1
-
-
-
2
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643807
Harris
Sulfur-deficient transfer ribo ...
Escherichia coli, Escherichia coli HfrC
Biochemistry
10
4207-4212
1971
-
-
-
-
-
-
-
2
-
1
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643805
Wong
Ribonucleic acid sulfurtransfe ...
Bacillus subtilis, Bacillus subtilis W168
Biochemistry
9
2376-2386
1970
-
-
-
-
-
-
1
2
-
1
-
2
-
4
-
-
1
-
-
-
1
-
13
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
1
1
2
-
1
-
2
-
-
-
1
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-