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Information on EC 2.7.11.17 - Ca2+/calmodulin-dependent protein kinase and Organism(s) Homo sapiens and UniProt Accession Q14012

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IUBMB Comments
Requires calmodulin and Ca2+ for activity. A wide range of proteins can act as acceptor, including vimentin, synapsin, glycogen synthase, myosin light chains and the microtubule-associated tau protein. Not identical with EC 2.7.11.18 (myosin-light-chain kinase) or EC 2.7.11.26 (tau-protein kinase).
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This record set is specific for:
Homo sapiens
UNIPROT: Q14012
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
caldesmon, cam kinase ii, camkiv, cam kinase, calcium/calmodulin-dependent protein kinase ii, calmodulin-dependent protein kinase, camkk2, calcium/calmodulin-dependent protein kinase, ca2+/calmodulin-dependent protein kinase, camk ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calcium/calmodulin-dependent kinase I
-
calcium/calmodulin-dependent protein kinase type I
-
CaMKI
alpha-calcium/calmodulin dependent protein kinase II
-
-
alpha-CaMKII
-
-
Ca2+/calmodulin-dependent protein kinase
-
-
Ca2+/calmodulin-dependent protein kinase Ialpha
-
-
Ca2+/calmodulin-dependent protein kinase II
Ca2+/calmodulin-dependent protein kinase IIalpha
-
-
Ca2+/calmodulin-dependent protein kinase IIdelta
-
Ca2+/calmodulin-dependent protein kinase IIgamma
-
Ca2+/calmodulin-dependent protein kinase kinase
-
Ca2+/calmodulin-dependent protein kinase kinase 2
-
CAKI
-
-
-
-
calcium-calmodulin dependent protein kinase IV
-
-
calcium-calmodulin-dependent protein kinase II
-
calcium-calmodulin-dependent protein kinase IV
-
calcium/calmodulin-dependent protein kinase
-
-
calcium/calmodulin-dependent protein kinase 1G
-
calcium/calmodulin-dependent protein kinase 2
-
-
calcium/calmodulin-dependent protein kinase II
-
-
calcium/calmodulin-dependent protein kinase II delta
-
calcium/calmodulin-dependent protein kinase IV
calcium/calmodulin-dependent protein kinase type II alpha chain
-
calcium/calmodulin-dependent protein kinase type II beta chain
-
calcium/calmodulin-dependent protein kinase type II delta chain
-
calcium/calmodulin-dependent protein kinase type IV catalytic chain
-
calcium/calmodulin-dependent serine protein kinase
-
-
caldesmon
-
-
-
-
calmodulin-dependent protein kinase
-
-
calmodulin-dependent protein kinase IV
-
-
Calspermin
-
-
-
-
CaM kinase
-
-
CaM kinase IG
-
CaM kinase II
-
-
CaM kinase IIalpha
-
-
CAM kinase-GR
-
-
-
-
CaM-dependent protein kinase
-
-
CaM-K I
-
-
CaM-K II
-
-
CaM-K IV
-
-
CaM-K1delta
-
CaM-kinase I
-
-
CaM-kinase II
-
-
CaM-kinase III
-
-
CaM-kinase IV
-
-
CaM-KIV
multifunctional enzyme
CaM-KKbeta3
-
CaMK IIalpha
-
CaMK IIbeta
-
CaMK IIdelta
-
CaMK IIgamma
-
CaMK-like CREB kinase-III
-
CaMK2
-
-
CaMKI
-
-
CaMKIgamma
-
CaMKII
CaMKIIalpha
-
-
CaMKIIdelta
isozyme
CaMKIIdeltaA
-
CaMKIIgamma
-
CaMKIIgammaB
-
CaMKIV
CASK
-
-
CLICK-III
-
CMPK
-
-
-
-
kinase, caldesmon (phosphorylating)
-
-
-
-
kinase, microtubule-associated protein 2 (phosphorylating)
-
-
-
-
MAP kinase
-
-
-
-
MAP-2 kinase
-
-
-
-
MAP-2 protein serine kinase
-
-
-
-
microtubule associated protein kinase
-
-
-
-
microtubule-associated protein 2 kinase
-
-
-
-
peripheral plasma membrane protein CaMGUK
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate
show the reaction diagram
sequential reaction mechanism in which all substrates bind to enzyme before any product is released. Binding of phospholamban to the enzyme decreases the affinity of the enzyme toward ATP
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (Ca2+/calmodulin-dependent)
Requires calmodulin and Ca2+ for activity. A wide range of proteins can act as acceptor, including vimentin, synapsin, glycogen synthase, myosin light chains and the microtubule-associated tau protein. Not identical with EC 2.7.11.18 (myosin-light-chain kinase) or EC 2.7.11.26 (tau-protein kinase).
CAS REGISTRY NUMBER
COMMENTARY hide
141467-21-2
-
93229-57-3
-
97350-82-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + synapsin I
ADP + phosphosynapsin I
show the reaction diagram
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
ATP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
ADP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-phospho-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
show the reaction diagram
biotinylated phospholamban Thr17 peptide substrate
-
-
?
ATP + Blc10
ADP + phosphorylated Blc10
show the reaction diagram
ATP + Ca2+/calmodulin-dependent protein kinase I
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase I
show the reaction diagram
ATP + Ca2+/calmodulin-dependent protein kinase IV
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase IV
show the reaction diagram
ATP + Cabin1
ADP + phosphorylated Cabin1
show the reaction diagram
ATP + cAMP-response element binding protein
ADP + phosphorylated cAMP-response element binding protein
show the reaction diagram
-
i.e. CREB, phosphorylation by CaMKII and CaMKIV at Ser133, and phosphorylation by CaMKII at Ser142
-
-
?
ATP + CARMA1
ADP + phosphorylated CARMA1
show the reaction diagram
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
i.e. cAMP-response element binding protein, substrate of CaM-KI, phosphorylation at Ser133
-
-
?
ATP + CREB1 protein
ADP + phosphorylated CREB1 protein
show the reaction diagram
-
-
-
?
ATP + eukaryotic translation initiation factor 4B
ADP + phosphorylated eukaryotic translation initiation factor 4B
show the reaction diagram
-
-
-
?
ATP + glutamate receptor subunit GluR1
ADP + phosphorylated glutamate receptor subunit GluR1
show the reaction diagram
ATP + glutamate receptor subunit NR2B
ADP + phosphorylated glutamate receptor subunit NR2B
show the reaction diagram
ATP + GST-tagged GluN2A
ADP + phosphorylated GST-tagged GluN2A
show the reaction diagram
-
-
-
-
?
ATP + GST-tagged GluN2B
ADP + phosphorylated GST-tagged GluN2B
show the reaction diagram
-
-
-
-
?
ATP + histone deacetylase 5
ADP + phosphorylated histone deacetylase 5
show the reaction diagram
-
-
-
?
ATP + I-kappaB
ADP + phosphorylated I-kappaB
show the reaction diagram
ATP + L-type Ca2+ channel
ADP + phosphorylated L-type Ca2+ channel
show the reaction diagram
ATP + NADPH oxidase 5
ADP + phosphoraletd NADPH oxidase 5
show the reaction diagram
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
?
ATP + protein PLB
ADP + phosphorylated protein PLB
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + protein RyR
ADP + phosphorylated protein RyR
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + PSD-95
ADP + phosphorylated PSD-95
show the reaction diagram
ATP + ryanodine receptor type 2
ADP + phosphorylated ryanodine receptor type 2
show the reaction diagram
-
i.e. RyR2
-
-
?
ATP + Stargazin
ADP + phosphorylated Stargazin
show the reaction diagram
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
?
ATP + stress-induced phosphoprotein 1
ADP + phosphorylated stress-induced phosphoprotein 1
show the reaction diagram
phosphorylated on Ser198
-
-
?
ATP + synapsin I
ADP + phosphorylated synapsin I
show the reaction diagram
-
substrate of CaM-KI
-
-
?
ATP + synapsin I
ADP + phosphosynapsin I
show the reaction diagram
-
-
-
?
ATP + synapsin II
ADP + phosphorylated synapsin II
show the reaction diagram
-
substrate of CaM-KI
-
-
?
ATP + synapsin-1
ADP + phopshorylated synapsin-1
show the reaction diagram
-
-
-
-
?
ATP + syntide-2
ADP + phosphorylated syntide-2
show the reaction diagram
-
-
-
?
ATP + tau peptide 408-420
ADP + phosphorylated tau peptide 408-420
show the reaction diagram
-
recombinant substrate expressed in Escherichia coli, phosphorylation at Ser416 by CaM kinase II, no activity with the peptide by CDK5, EC 2.7.11.22, and casein kinase II, EC 2.7.11.26
-
-
?
ATP + tau protein
ADP + phosphorylated tau protein
show the reaction diagram
ATP + type alpha cytosolic phospholipase A2
ADP + phosphorylated type alpha cytosolic phospholipase A2
show the reaction diagram
-
CaMK regulates the type alpha cytosolic phospholipase A2 via phosphorylation at Ser515
-
-
?
ATP + vimentin
ADP + phosphorylated vimentin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
the enzyme (CaMKK2) directly phosphorylates Akt at Thr308
-
-
?
ATP + Blc10
ADP + phosphorylated Blc10
show the reaction diagram
-
Bcl10 is essential for antigen receptor-induced NF-kappaB activation, interleukin-2 production, and T-cell proliferation but is not required for TCR-induced tyrosine phosphorylation, calcium flux, or extracellular signal-regulated kinase activation
-
-
?
ATP + Ca2+/calmodulin-dependent protein kinase I
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase I
show the reaction diagram
the enzyme is a member of the CaMK cascade that mediates the response to intracellular Ca2+ elevation. Ca2+/calmodulin-dependent protein kinase kinase phosphorylates and activates Ca2+/calmodulin-dependent protein kinase I and Ca2+/calmodulin-dependent protein kinase IV, which directly activate transcription factors
-
-
?
ATP + Ca2+/calmodulin-dependent protein kinase IV
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase IV
show the reaction diagram
the enzyme is a member of the CaMK cascade that mediates the response to intracellular Ca2+ elevation. Ca2+/calmodulin-dependent protein kinase kinase phosphorylates and activates Ca2+/calmodulin-dependent protein kinase I and Ca2+/calmodulin-dependent protein kinase IV, which directly activate transcription factors
-
-
?
ATP + Cabin1
ADP + phosphorylated Cabin1
show the reaction diagram
-
a transcriptional corepressor of myocyte enhancer factor 2, phosphorylation by CaMKIV creates a docking site for protein 14-3-3, which causes nuclear export, CaMKIV regulates nuclear export of Cabin1 during Ca2+-dependent T-cell activation, regulation overview
-
-
?
ATP + cAMP-response element binding protein
ADP + phosphorylated cAMP-response element binding protein
show the reaction diagram
-
i.e. CREB, phosphorylation by CaMKII and CaMKIV at Ser133, and phosphorylation by CaMKII at Ser142
-
-
?
ATP + CARMA1
ADP + phosphorylated CARMA1
show the reaction diagram
-
CaMKII is a modulator of CARMA1-mediated NF-kappaB activation, overview
-
-
?
ATP + CREB1 protein
ADP + phosphorylated CREB1 protein
show the reaction diagram
-
-
-
?
ATP + glutamate receptor subunit GluR1
ADP + phosphorylated glutamate receptor subunit GluR1
show the reaction diagram
-
low activity, CaMKII, membrane localization of GluR1 restricts its phosphorylation
-
-
?
ATP + glutamate receptor subunit NR2B
ADP + phosphorylated glutamate receptor subunit NR2B
show the reaction diagram
-
low activity, CaMKII
-
-
?
ATP + I-kappaB
ADP + phosphorylated I-kappaB
show the reaction diagram
-
substrate of CaM-KII in T-lymphocytes and neurons, phosphorylation leads to activation of I-kappa B, which mediates activation of NF-kappaB
-
-
?
ATP + L-type Ca2+ channel
ADP + phosphorylated L-type Ca2+ channel
show the reaction diagram
-
inactivation, leading to increased Ca2+ channel open probability and increased arrhytmias
-
-
?
ATP + NADPH oxidase 5
ADP + phosphoraletd NADPH oxidase 5
show the reaction diagram
-
the enzyme mediates the phosphorylation and activation of NADPH oxidase 5. The ability of Ca2+/calmodulin-dependent protein kinase to regulate NADPH oxidase 5 activity may be significant in the regulation of production of reactive oxygen species that occurs downstream of calcium-mobilizing agonists such as angiotensin II. Of these phosphorylation sites, mutation of only Ser475 to alanine prevents Ca2+/calmodulin-dependent protein kinase-induced increases in activity of NADPH oxidase 5
-
-
?
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
?
ATP + protein PLB
ADP + phosphorylated protein PLB
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + protein RyR
ADP + phosphorylated protein RyR
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + PSD-95
ADP + phosphorylated PSD-95
show the reaction diagram
-
high activity, CaMKII, smaller synapses show greater variability in PSD-95 phosphorylation
-
-
?
ATP + ryanodine receptor type 2
ADP + phosphorylated ryanodine receptor type 2
show the reaction diagram
-
i.e. RyR2
-
-
?
ATP + Stargazin
ADP + phosphorylated Stargazin
show the reaction diagram
-
high activity, CaMKII, phosphatases limit phosphorylation of stargazin
-
-
?
ATP + synapsin-1
ADP + phopshorylated synapsin-1
show the reaction diagram
-
-
-
-
?
ATP + tau protein
ADP + phosphorylated tau protein
show the reaction diagram
-
phosphorylation at Ser416 by CaM kinase II is high in early stages of brain development, CaM kinase II is involved in the accumulation of tau in neuronal soma in Alzheimer's disease brain
-
-
?
ATP + vimentin
ADP + phosphorylated vimentin
show the reaction diagram
-
specific phosphorylation by CaMKII at Ser82
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
dependent on
Calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaMK phosphatase
specifically dephosphorylates and regulates activity
-
CaMKI (294-321)
specific CaMKI inhibitor
-
CaMKP phosphatase-N
specifically dephosphorylates and regulates activity
-
KN-62
specific CaMK inhibitor
KN-93
protein phosphatase 1
negative CaMK regulation by dephosphorylation
-
Protein phosphatase 2A
negative CaMK regulation by dephosphorylation
-
protein phosphatase 2B
calcineurin, negative CaMK regulation by dephosphorylation
-
protein phosphatase 2C
negative CaMK regulation by dephosphorylation
-
protein phosphatase M
negative CaMK regulation by dephosphorylation
-
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
-
-
2-[4-[6-(quinolin-8-yloxy)pyrimidin-4-yl]piperazin-1-yl]benzonitrile
-
-
-
2-[N-(2-hydroxyethyl)-N-(4-methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine
-
KN-93
3-([6-(quinolin-8-yloxy)pyrimidin-4-yl]oxy)aniline
-
-
4-methyl-7-([6-[(quinolin-8-yl)oxy]pyrimidin-4-yl]oxy)-2H-1-benzopyran-2-one
-
6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]-N-(4-methoxyphenyl)pyrimidin-4-amine
-
7-([6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]pyrimidin-4-yl]oxy)-4-methyl-2H-1-benzopyran-2-one
-
7-[(6-chloropyrimidin-4-yl)oxy]-4-methyl-2H-1-benzopyran-2-one
-
7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate
i.e. STO-609. The CaMKKbeta residue Pro274, which replaces the conserved acidic residue of other protein kinases, is an important determinant for the selective inhibition by STO-609. ATP-competitive inhibition mechanism
8-([6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]pyrimidin-4-yl]oxy)quinoline
-
8-([6-[(naphthalen-2-yl)oxy]pyrimidin-4-yl]oxy)-1,4-dihydroquinoline
-
8-[(6-chloropyrimidin-4-yl)oxy]quinoline
-
AC3-I
specific CaMKII inhibitor
AIP
specific CaMKII inhibitor
autocamide-2-related inhibitory peptide
-
i.e. AIP, used in its myristoylated form
-
autocamtide-2 related inhibitory peptide
-
-
-
autocamtide-2-related inhibitory peptide
-
specific and potent CaMKII inhibitor
-
BAPTA-AM
-
-
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
Calmidazolium
-
calmodulin antagonist
CaM-KIIN
-
noncompetitive inhibitor
-
CaMK phosphatase
specifically dephosphorylates and regulates activity
-
CaMKII (273-302)
specific CaMKII inhibitor
-
CaMKII (281-309)
specific CaMKII inhibitor
-
CaMKIIN
-
brain-specific CaMKII inhibitor
-
CaMKP phosphatase-N
specifically dephosphorylates and regulates activity
-
CN-21
specific CaMKII inhibitor
curcumin
curcumin strongly interacts with isoform CAMK4
imatinib
downregulates CaMKIIgamma autophosphorylation/activation
K252a
-
inhibits CaM-KII
KN-62
KN-93
KN62
-
inhibits CaMKII
methyl-beta-cyclodextrin
-
-
N-(4-methoxyphenyl)-6-(piperazin-1-yl)pyrimidin-4-amine
-
-
pea protein peptides
-
purified positively charged peptides produced from pea cellular proteins by alkaline protease, i.e. alcalase, are inhibitory for CaMKII in a competitive manner, especially those with a high content of lysine and arginine, which act more in a mixed inhibition type, amino acid compositions of the peptides and IC50 of peptide fractions, overview
-
PEP-19
specific CaMKII inhibitor
-
peptide AC3-I
-
i.e. autocamtide-2 inhibitory peptide, inhibits CaMKII
peptide AIP
-
i.e. autocamtide-2-related inhibitory peptide, comprising 17 amino acids, a CaMKII inhibitory peptide with the sequence KKALRRQEAVDAL
protein phosphatase 1
negative CaMK regulation by dephosphorylation
-
Protein phosphatase 2A
negative CaMK regulation by dephosphorylation
-
protein phosphatase 2B
calcineurin, negative CaMK regulation by dephosphorylation
-
protein phosphatase 2C
negative CaMK regulation by dephosphorylation
-
protein phosphatase M
negative CaMK regulation by dephosphorylation
-
STO-609
trifluoperazine dimaleate
-
calmodulin antagonist
W-13
-
calmodulin antagonist
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
dependent on, intracellular calcium receptor
7-oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylic acid
-
STO-609, triggers the increase of Ca2+ and activation of Ca2+/calmodulin-dependent protein kinase Ialpha
Ca2+/calmodulin-dependent protein kinase kinase
-
-
Calmodulin
ionomycin
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0071 - 178.29
ATP
0.0064 - 0.0158
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
6.05 - 11.75
GST-tagged GluN2A
-
0.35 - 5.93
GST-tagged GluN2B
-
additional information
additional information
-
kinetics for binding of calmodulin
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 9.9
ATP
1.2 - 9.9
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
0.17 - 0.33
GST-tagged GluN2A
-
0.16 - 0.82
GST-tagged GluN2B
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004 - 0.32
ATP
0.01 - 0.05
GST-tagged GluN2A
-
0.16 - 0.56
GST-tagged GluN2B
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
inhibition kinetics for positively charged pea protein-derived peptides
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.104
2-[4-[6-(quinolin-8-yloxy)pyrimidin-4-yl]piperazin-1-yl]benzonitrile
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37°C
-
0.116
3-([6-(quinolin-8-yloxy)pyrimidin-4-yl]oxy)aniline
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37°C
0.072 - 0.35
4-methyl-7-([6-[(quinolin-8-yl)oxy]pyrimidin-4-yl]oxy)-2H-1-benzopyran-2-one
0.06
6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]-N-(4-methoxyphenyl)pyrimidin-4-amine
Homo sapiens
pH and temperature not specified in the publication, for HuH7 cells: 0.06 mM
0.054
7-([6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]pyrimidin-4-yl]oxy)-4-methyl-2H-1-benzopyran-2-one
Homo sapiens
pH and temperature not specified in the publication, for HuH7 cells
0.124 - 0.33
7-[(6-chloropyrimidin-4-yl)oxy]-4-methyl-2H-1-benzopyran-2-one
0.02 - 0.5
7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate
0.04
8-([6-[(5-methoxy-1H-benzimidazol-2-yl)sulfanyl]pyrimidin-4-yl]oxy)quinoline
Homo sapiens
pH and temperature not specified in the publication, for HuH7 cells: 0.04 mM
0.055 - 0.38
8-([6-[(naphthalen-2-yl)oxy]pyrimidin-4-yl]oxy)-1,4-dihydroquinoline
0.039
8-[(6-chloropyrimidin-4-yl)oxy]quinoline
Homo sapiens
pH and temperature not specified in the publication, for HuH7 cells
0.046
N-(4-methoxyphenyl)-6-(piperazin-1-yl)pyrimidin-4-amine
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004 - 0.022
-
recombinant nonphosphorylated, calmodulin-binding-residue mutants
0.035
purified recombinant CaM-K1delta
0.98
purified recombinant CaM-K1delta phosphorylated by CaM-KKalpha
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11.5
within the pH range, CAMKIV maintains both its secondary and tertiary structures, along with its function, whereas significant aggregation is observed at acidic pH 2.0-4.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
24
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
differential expression of CaM-KI and CaM-KII in cell lines MCF-7 and MCF-10A
Manually annotated by BRENDA team
breast cancer cell line
Manually annotated by BRENDA team
breast cancer cell line
Manually annotated by BRENDA team
very low level
Manually annotated by BRENDA team
-
from heart failure
Manually annotated by BRENDA team
Jurkat cell
Manually annotated by BRENDA team
-
CaM-KI
Manually annotated by BRENDA team
low expression level of CaM-K1delta
Manually annotated by BRENDA team
low expression level of CaM-K1delta
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
neuron
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
-
in human hypertrophy, both CaMKII and PKA functionally regulate RyR2 and may induce SR Ca2+ leak. In the transition from hypertrophy to heart failure, the diastolic Ca2+ leak increases and disturbed Ca2+ cycling occurs. This is associated with an increase in CaMKII- but not PKA-dependent RyR2 phosphorylation. PKA-dependent RyR2 phosphorylation is not increased in heart failure and is independent of previous beta-blocker treatment. In heart failure, CaMKII inhibition but not inhibition of PKA yields a reduction of the SR Ca2+ leak. PKA inhibition further reduced SR Ca2+ load and systolic Ca2+ transients. CaMKII inhibition may thus reflect a promising therapeutic target for the treatment of arrhythmias and contractile dysfunction
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KCC1A_HUMAN
370
0
41337
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
isozyme CaMKI
51925
x * 51925, calculation from nucleotide sequence
550000
isozyme CaMKII
60000
isozyme CaMKIV
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 40000, isozyme CaMKI
?
x * 51925, calculation from nucleotide sequence
monomer
multimer
oligomer
isozyme CaMKII
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
CaMKI and CaMKII are phosphorylated by the calcium/calmodulin-dependent kinase kinase CaM-KK isozymes, regulatory role, overview
glycoprotein
the enzyme has seven glycosylation sites at Thr57, Ser58, Ser137, Ser189, Ser344, Ser345 and Ser356
phosphoprotein
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method at 20°C, crystal structure of the catalytic kinase domain of the human CaMKKbeta isoform complexed with its selective inhibitor 7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate (i.e. STO-609). The crystal belongs to the primitive orthorhombic space group P2(1)2(1)2(1) with unit cell constants of a = 69.4 A, b = 77.2 A, c = 84.3 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E60G
-
the mutation exerts different effects on the kinetic parameters of phosphorylation of GluN2B and GluN2A, of which only GluN2B binds to the T-site of the enzyme. GluN2B-induced modulation of the kinetic parameters of peptide substrate is altered in the mutant. The mutation almost abolishes the modulation of the apparent Km value for protein substrate. When GST-tagged GluN2A is used as the substrate, the rate of phosphorylation of the mutant decreases considerably as seen by a 4.3fold reduction in kcat in comparison with that of wild type enzyme. The mutant does not exhibit calcium-independent activity
F320D/N321D
-
site-directed mutagenesis, the mutation changes the sequence FNDD to DDDD, the mutant shows no binding of calmodulin and increased activity in nonphosphorylated status compared to the wild-type enzyme
I205K
-
site-directed mutagenesis, the mutant CaMKII shows reduced activity with vimentin compared to the wild-type enzyme
K75E
-
site-directed mutagenesis, catalytically inactive CaMKIV mutant
P274E
the P274E mutation significantly reduces the inhibitory effect of 7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate (i.e. STO-609) on the isoenzyme CaMKKbeta
T180A
site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme, the mutant is phosphorylated and activated by CaM-KKalpha
T200A
-
site-directed mutagenesis, the mutant CaMKIV is not phosphorylated by CaMKKbeta and is thus incapable of developing autonomous activity and control gene transcription, the same effect occurs due to combination of T200A with calmodulin binding site mutations, overview
T287D
constitutively active CaMKIId mutant
T287D/K43M
kinase dead CaMKIId mutant
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
CaMKIIalpha clusters associated with lipid raft fraction are resistant to treatment with methyl-beta-cyclodextrin and subsequent cold Triton X-100, which suggests the stabilization of CaMKIIalpha cluster-associated lipid rafts
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, NiNTA resin column chromatography, Ni-HF affinity gel filtration, and Sephacryl S-200 gel filtration
ion-exchange chromatography and affinity chromatography
-
recombinant FLAG-tagged CaMKIV from 293A cells
-
recombinant functionally active CAMKIV kinase domain
recombinant GST-tagged wild-type and mutant CaM-K1delta from Escherichia coli by glutathione affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CaM-K1delta, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzyme in Escherichia coli strain JM109, transient expression of HA-tagged CaM-K1delta 1-296 Ca2+/calmodulin-independent mutant in HeLa and COS-7 cells
CaMKIgamma, DNA and amino acid sequence determination and analysis, expression in COS-7 cells
cloning and recombinant expression of functionally active CAMKIV kinase domain
coexpression of GST-tagged enzyme with GST-tagged Cav2.1 channels in transfected tsA-201 cells and GST affinity pulldown assay, recombinant expression of MBP-fusion enzyme in Escherichia coli strain BL21
-
expressed in a wheat cell-free system
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in GT1-7 cells
-
expressed in Sf21 or High Five insect cells
-
expressed in the LXSN retroviral expression vector M28248
expression in Escherichia coli
expression in Jurkat cells, co-expression with human CARMA1, T-cell receptor engagement redistributes CaMKII to the immune synapse in an activation-dependent manner
-
expression of CaMKII in HEK-293 cells, and expression of CaMKII in cultured neurons using lentiviral transfection, co-expression of protein substrates
-
expression of FLAG-tagged CaMKIV in 293A cells
-
functional cytoplasmic co-expression of wild-type CaMKII with vimentin-NR2B, a construct of a vimentin peptide containing Ser82 fused to the NMDA receptor 2B subunit, an interaction partner of CaMKII, in HEK-293 cells, translocation of the Vin-NR2B construct to the membrane is regulated by lipid rafts resulting in reduced phosphorylation by CaMKII
-
isolation and sequencing of cDNA
recombinant expression in TsA-201 cells, the recombinant enzyme interacts and co-localizes with the presynaptic N-type voltage-dependent calcium channel (Cav2.2) and induces in TsA-201 cell line an increase of calcium current mediated by this channel
-
transient co-expression of CaMKIV, MEF-2, and Cabin1 in murine T-cell hybridoma DO11.10, MEF-2 activation of gene transcription is inhibited by Cabin1, the inhibition is reversed by CaMKIV
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
highly expressed in high-grade serous ovarian cancer
intracellular levels of phosphorylated and total calcium/calmodulin kinase 2 are elevated following basic calcium phosphate treatment
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Ca2+/calmodulin-dependent protein kinases may be involved in cancer, cardiac hypertrophy, heart failure, arrhythmia, cardiomyocyte apoptosis, the regulation of higher order neuronal functions such as memory, learning disorder, Angelman's syndrome, Parkinson's disease, Alzheimer's disease, delayed neuronal death, diabetes, and osteoporosis
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q13554), Homo sapiens (Q9UQM7), Metarhizium anisopliae (O14408), Mus musculus (Q923T9)
Manually annotated by BRENDA team
Rochlitz, H.; Voigt, A.; Lankat-Buttgereit, B.; Goke, B.; Heimberg, H.; Nauck, M.A.; Schiemann, U.; Schatz, H.; Pfeiffer, A.F.
Cloning and quantitative determination of the human Ca2+/calmodulin-dependent protein kinase II (CaMK II) isoforms in human beta cells
Diabetologia
43
465-473
2000
Homo sapiens (Q13554), Homo sapiens
Manually annotated by BRENDA team
Tombes, R.M.; Krystal, G.W.
Identification of novel human tumor cell-specific CaMK-II variants
Biochim. Biophys. Acta
1355
281-292
1997
Homo sapiens (Q13554), Homo sapiens (Q13557)
Manually annotated by BRENDA team
Wang, P.; Wu, Y.L.; Zhou, T.H.; Sun, Y.; Pei, G.
Identification of alternative splicing variants of the beta subunit of human Ca(2+)/calmodulin-dependent protein kinase II with different activities
FEBS Lett.
475
107-110
2000
Homo sapiens (Q13554)
Manually annotated by BRENDA team
Hoch, B.; Meyer, R.; Hetzer, R.; Krause, E.G.; Karczewski, P.
Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium
Circ. Res.
84
713-721
1999
Homo sapiens (Q13557), Homo sapiens
Manually annotated by BRENDA team
Haribabu, B.; Hook, S.S.; Selbert, M.A.; Goldstein, E.G.; Tomhave, E.D.; Edelman, A.M.; Snyderman, R.; Means, A.R.
Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase
EMBO J.
14
3679-3686
1995
Homo sapiens (Q14012), Homo sapiens
Manually annotated by BRENDA team
Bland, M.M.; Monroe, R.S.; Ohmstede, C.A.
The cDNA sequence and characterization of the Ca2+/calmodulin-dependent protein kinase-Gr from human brain and thymus
Gene
142
191-197
1994
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Kitani, T.; Okuno, S.; Fujisawa, H.
cDNA cloning and expression of human calmodulin-dependent protein kinase IV
J. Biochem.
115
637-640
1994
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Mosialos, G.; Hanissian, S.H.; Jawahar, S.; Vara, L.; Kieff, E.; Chatila, T.A.
A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1
J. Virol.
68
1697-1705
1994
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Rodriguez-Mora, O.G.; LaHair, M.M.; McCubrey, J.A.; Franklin, R.A.
Calcium/calmodulin-dependent kinase I and calcium/calmodulin-dependent kinase kinase participate in the control of cell cycle progression in MCF-7 human breast cancer cells
Cancer Res.
65
5408-5416
2005
Homo sapiens (Q14012), Homo sapiens
Manually annotated by BRENDA team
Zhang, T.; Brown, J.H.
Role of Ca2+/calmodulin-dependent protein kinase II in cardiac hypertrophy and heart failure
Cardiovasc. Res.
63
476-486
2004
Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Pan, F.; Means, A.R.; Liu, J.O.
Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
EMBO J.
24
2104-2113
2005
Homo sapiens
Manually annotated by BRENDA team
Rodriguez-Mora, O.; LaHair, M.M.; Howe, C.J.; McCubrey, J.A.; Franklin, R.A.
Calcium/calmodulin-dependent protein kinases as potential targets in cancer therapy
Expert Opin. Ther. Targets
9
791-808
2005
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Ishikawa, Y.; Tokumitsu, H.; Inuzuka, H.; Murata-Hori, M.; Hosoya, H.; Kobayashi, R.
Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells
FEBS Lett.
550
57-63
2003
Homo sapiens (Q8IU85)
Manually annotated by BRENDA team
Takemoto-Kimura, S.; Terai, H.; Takamoto, M.; Ohmae, S.; Kikumura, S.; Segi, E.; Arakawa, Y.; Furuyashiki, T.; Narumiya, S.; Bito, H.
Molecular cloning and characterization of CLICK-III/CaMKIg, a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK)
J. Biol. Chem.
278
18597-18605
2003
Rattus norvegicus, Mus musculus (Q91VB2), Homo sapiens (Q96NX5)
Manually annotated by BRENDA team
Chow, F.A.; Anderson, K.A.; Noeldner, P.K.; Means, A.R.
The autonomous activity of calcium/calmodulin-dependent protein kinase IV is required for its role in transcription
J. Biol. Chem.
280
20530-20538
2005
Homo sapiens
Manually annotated by BRENDA team
Tsui, J.; Inagaki, M.; Schulman, H.
Calcium/calmodulin-dependent protein kinase II (CaMKII) localization acts in concert with substrate targeting to create spatial restriction for phosphorylation
J. Biol. Chem.
280
9210-9216
2005
Homo sapiens
Manually annotated by BRENDA team
Yamamoto, H.; Hiragami, Y.; Murayama, M.; Ishizuka, K.; Kawahara, M.; Takashima, A.
Phosphorylation of tau at serine 416 by Ca2+/calmodulin-dependent protein kinase II in neuronal soma in brain
J. Neurochem.
94
1438-1447
2005
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Li, H.; Aluko, R.E.
Kinetics of the inhibition of calcium/calmodulin-dependent protein kinase II by pea protein-derived peptides
J. Nutr. Biochem.
16
656-662
2005
Homo sapiens
Manually annotated by BRENDA team
Maier, L.S.; Bers, D.M.
Role of Ca2+/calmodulin-dependent protein kinase (CaMK) in excitation-contraction coupling in the heart
Cardiovasc. Res.
73
631-640
2007
Oryctolagus cuniculus, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Tsui, J.; Malenka, R.C.
Substrate localization creates specificity in calcium/calmodulin-dependent protein kinase II signaling at synapses
J. Biol. Chem.
281
13794-13804
2006
Homo sapiens
Manually annotated by BRENDA team
Ishiguro, K.; Green, T.; Rapley, J.; Wachtel, H.; Giallourakis, C.; Landry, A.; Cao, Z.; Lu, N.; Takafumi, A.; Goto, H.; Daly, M.J.; Xavier, R.J.
Ca2+/calmodulin-dependent protein kinase II is a modulator of CARMA1-mediated NF-kappaB activation
Mol. Cell. Biol.
26
5497-5508
2006
Homo sapiens
Manually annotated by BRENDA team
Ishida, A.; Sueyoshi, N.; Shigeri, Y.; Kameshita, I.
Negative regulation of multifunctional Ca2+/calmodulin-dependent protein kinases: physiological and pharmacological significance of protein phosphatases
Br. J. Pharmacol.
154
729-740
2008
Homo sapiens, Homo sapiens (Q14012), Homo sapiens (Q16566)
Manually annotated by BRENDA team
Si, J.; Collins, S.J.
Activated Ca2+/calmodulin-dependent protein kinase IIgamma is a critical regulator of myeloid leukemia cell proliferation
Cancer Res.
68
3733-3742
2008
Homo sapiens (Q13555)
Manually annotated by BRENDA team
Shimizu, M.; Nakamura, H.; Hirabayashi, T.; Suganami, A.; Tamura, Y.; Murayama, T.
Ser515 phosphorylation-independent regulation of cytosolic phospholipase A2alpha (cPLA2alpha) by calmodulin-dependent protein kinase: possible interaction with catalytic domain A of cPLA2alpha
Cell. Signal.
20
815-824
2008
Homo sapiens
Manually annotated by BRENDA team
Bossuyt, J.; Helmstadter, K.; Wu, X.; Clements-Jewery, H.; Haworth, R.S.; Avkiran, M.; Martin, J.L.; Pogwizd, S.M.; Bers, D.M.
Ca2+/calmodulin-dependent protein kinase IIdelta and protein kinase D overexpression reinforce the histone deacetylase 5 redistribution in heart failure
Circ. Res.
102
695-702
2008
Oryctolagus cuniculus (O77708), Oryctolagus cuniculus, Homo sapiens (Q13557), Homo sapiens
Manually annotated by BRENDA team
Matsumoto, K.; Murao, K.; Imachi, H.; Nishiuchi, T.; Cao, W.; Yu, X.; Li, J.; Ahmed, R.A.; Iwama, H.; Kobayashi, R.; Tokumitsu, H.; Ishida, T.
The role of calcium/calmodulin-dependent protein kinase cascade on MIP-1alpha gene expression of ATL cells
Exp. Hematol.
36
390-400
2008
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Masaoka, T.; Nishi, M.; Ryo, A.; Endo, Y.; Sawasaki, T.
The wheat germ cell-free based screening of protein substrates of calcium/calmodulin-dependent protein kinase II delta
FEBS Lett.
582
1795-1801
2008
Homo sapiens (Q13557), Homo sapiens
Manually annotated by BRENDA team
Wang, Y.; Tandan, S.; Cheng, J.; Yang, C.; Nguyen, L.; Sugianto, J.; Johnstone, J.L.; Sun, Y.; Hill, J.A.
Ca2+/calmodulin-dependent protein kinase II-dependent remodeling of Ca2+ current in pressure overload heart failure
J. Biol. Chem.
283
25524-25532
2008
Homo sapiens
Manually annotated by BRENDA team
Suzuki, T.; Du, F.; Tian, Q.B.; Zhang, J.; Endo, S.
Ca2+/calmodulin-dependent protein kinase IIalpha clusters are associated with stable lipid rafts and their formation traps PSD-95
J. Neurochem.
104
596-610
2008
Homo sapiens
Manually annotated by BRENDA team
Jiang, X.; Lautermilch, N.J.; Watari, H.; Westenbroek, R.E.; Scheuer, T.; Catterall, W.A.
Modulation of CaV2.1 channels by Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain
Proc. Natl. Acad. Sci. USA
105
341-346
2008
Homo sapiens
Manually annotated by BRENDA team
Monteiro, P.; Gilot, D.; Langouet, S.; Fardel, O.
Activation of the aryl hydrocarbon receptor by the calcium/calmodulin-dependent protein kinase kinase inhibitor 7-oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylic acid (STO-609)
Drug Metab. Dispos.
36
2556-2563
2008
Homo sapiens
Manually annotated by BRENDA team
Huynh, Q.K.; Pagratis, N.
Kinetic mechanisms of Ca++/calmodulin dependent protein kinases
Arch. Biochem. Biophys.
506
130-136
2011
Homo sapiens (Q13554), Homo sapiens (Q13555), Homo sapiens (Q13557), Homo sapiens (Q9UQM7)
Manually annotated by BRENDA team
Hoffman, L.; Farley, M.M.; Waxham, M.N.
Calcium-calmodulin-dependent protein kinase II isoforms differentially impact the dynamics and structure of the actin cytoskeleton
Biochemistry
52
1198-1207
2013
Rattus norvegicus (P08413), Rattus norvegicus (P11275), Homo sapiens (Q13555), Homo sapiens (Q13557)
Manually annotated by BRENDA team
Kukimoto-Niino, M.; Yoshikawa, S.; Takagi, T.; Ohsawa, N.; Tomabechi, Y.; Terada, T.; Shirouzu, M.; Suzuki, A.; Lee, S.; Yamauchi, T.; Okada-Iwabu, M.; Iwabu, M.; Kadowaki, T.; Minokoshi, Y.; Yokoyama, S.
Crystal structure of the Ca2+/calmodulin-dependent protein kinase kinase in complex with the inhibitor STO-609
J. Biol. Chem.
286
22570-22579
2011
Homo sapiens (Q96RR4), Homo sapiens
Manually annotated by BRENDA team
Pandey, D.; Gratton, J.P.; Rafikov, R.; Black, S.M.; Fulton, D.J.
Calcium/calmodulin-dependent kinase II mediates the phosphorylation and activation of NADPH oxidase 5
Mol. Pharmacol.
80
407-415
2011
Homo sapiens
Manually annotated by BRENDA team
Naz, H.; Shahbaaz, M.; Bisetty, K.; Islam, A.; Ahmad, F.; Hassan, M.I.
Effect of pH on the structure, function, and stability of human calcium/calmodulin-dependent protein kinase IV: combined spectroscopic and MD simulation studies
Biochem. Cell Biol.
94
221-228
2016
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Naz, H.; Jameel, E.; Hoda, N.; Shandilya, A.; Khan, P.; Islam, A.; Ahmad, F.; Jayaram, B.; Hassan, M.I.
Structure guided design of potential inhibitors of human calcium-calmodulin dependent protein kinase IV containing pyrimidine scaffold
Bioorg. Med. Chem. Lett.
26
782-788
2016
Homo sapiens
Manually annotated by BRENDA team
Nafzger, S.; Rougier, J.S.
Calcium/calmodulin-dependent serine protein kinase CASK modulates the L-type calcium current
Cell Calcium
61
10-21
2016
Homo sapiens
Manually annotated by BRENDA team
Fischer, T.H.; Herting, J.; Tirilomis, T.; Renner, A.; Neef, S.; Toischer, K.; Ellenberger, D.; Foerster, A.; Schmitto, J.D.; Gummert, J.; Schoendube, F.A.; Hasenfuss, G.; Maier, L.S.; Sossalla, S.
Ca2+/calmodulin-dependent protein kinase II and protein kinase A differentially regulate sarcoplasmic reticulum Ca2+ leak in human cardiac pathology
Circulation
128
970-981
2013
Homo sapiens
Manually annotated by BRENDA team
Magupalli, V.; Mochida, S.; Yan, J.; Jiang, X.; Westenbroek, R.; Nairn, A.; Scheuer, T.; Catterall, W.
Ca2+-independent activation of Ca2+/Calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels
J. Biol. Chem.
288
4637-4648
2013
Homo sapiens
Manually annotated by BRENDA team
Madhavan, M.; Mohanan, A.G.; Jacob, R.S.; Gunasekaran, S.; Nair, R.R.; Omkumar, R.V.
Glu60 of alpha-calcium/calmodulin dependent protein kinase II mediates crosstalk between the regulatory T-site and protein substrate binding region of the active site
Arch. Biochem. Biophys.
685
108348
2020
Homo sapiens
Manually annotated by BRENDA team
Rong, J.; Pool, B.; Zhu, M.; Munro, J.; Cornish, J.; McCarthy, G.M.; Dalbeth, N.; Poulsen, R.
Basic calcium phosphate crystals induce osteoarthritis-associated changes in phenotype markers in primary human chondrocytes by a calcium/calmodulin kinase 2-dependent mechanism
Calcif. Tissue Int.
104
331-343
2019
Homo sapiens
Manually annotated by BRENDA team
Jameel, E.; Naz, H.; Khan, P.; Tarique, M.; Kumar, J.; Mumtazuddin, S.; Ahamad, S.; Islam, A.; Ahmad, F.; Hoda, N.; Hassan, M.
Design, synthesis, and biological evaluation of pyrimidine derivatives as potential inhibitors of human calcium/calmodulin-dependent protein kinase IV
Chem. Biol. Drug Des.
89
741-754
2017
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team
Gocher, A.; Azabdaftari, G.; Euscher, L.; Dai, S.; Karacosta, L.; Franke, T.; Edelman, A.
Akt activation by Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2) in ovarian cancer cells
J. Biol. Chem.
292
14188-14204
2017
Homo sapiens (Q96RR4)
Manually annotated by BRENDA team
Hoda, N.; Naz, H.; Jameel, E.; Shandilya, A.; Dey, S.; Hassan, M.; Ahmad, F.; Jayaram, B.
Curcumin specifically binds to the human calcium-calmodulin-dependent protein kinase IV Fluorescence and molecular dynamics simulation studies
J. Biomol. Struct. Dyn.
34
572-584
2016
Homo sapiens (Q16566), Homo sapiens
Manually annotated by BRENDA team