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Literature summary for 2.7.11.17 extracted from

  • Huynh, Q.K.; Pagratis, N.
    Kinetic mechanisms of Ca++/calmodulin dependent protein kinases (2011), Arch. Biochem. Biophys., 506, 130-136.
    View publication on PubMed
Search for more literature for 2.7.11.17

Inhibitors

Inhibitors Comment Organism Structure
ADP product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIalpha; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIbeta; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIdelta; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIgamma Homo sapiens
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIalpha; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIbeta; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIdelta; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIgamma Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIdelta Homo sapiens
0.0071
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIalpha Homo sapiens
0.0072
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIdelta Homo sapiens
0.0084
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIgamma Homo sapiens
0.0097
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIalpha Homo sapiens
0.0128
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIgamma Homo sapiens
0.0158
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIbeta Homo sapiens
0.0259
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIbeta Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phospholamban Homo sapiens
-
 ADP + phosphorylated phospholamban
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13554
-
-
Homo sapiens Q13555
-
-
Homo sapiens Q13557
-
-
Homo sapiens Q9UQM7
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate sequential reaction mechanism in which all substrates bind to enzyme before any product is released. Binding of phospholamban to the enzyme decreases the affinity of the enzyme toward ATP Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln biotinylated phospholamban Thr17 peptide substrate Homo sapiens ADP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-phospho-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
-
 ?
ATP + phospholamban
-
 Homo sapiens ADP + phosphorylated phospholamban
-
 ?

Synonyms

Synonyms Comment Organism
CaMK IIalpha
-
 Homo sapiens
CaMK IIbeta
-
 Homo sapiens
CaMK IIdelta
-
 Homo sapiens
CaMK IIgamma
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIalpha Homo sapiens
1.2
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIalpha Homo sapiens
8
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIbeta Homo sapiens
8
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIbeta Homo sapiens
9.8
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIgamma Homo sapiens
9.8
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIgamma Homo sapiens
9.9
-
 ATP pH 7.6, 25°C, isoenzyme CaMK IIdelta Homo sapiens
9.9
-
 biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln pH 7.6, 25°C, isoenzyme CaMK IIdelta Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens