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Literature summary for 2.7.11.17 extracted from

  • Ishikawa, Y.; Tokumitsu, H.; Inuzuka, H.; Murata-Hori, M.; Hosoya, H.; Kobayashi, R.
    Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells (2003), FEBS Lett., 550, 57-63.
    View publication on PubMed
Search for more literature for 2.7.11.17

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin absolutely dependent on Homo sapiens
ionomycin activates the Ca2+/calmodulin-activated CaM-K1delta further 1.5fold Homo sapiens
additional information CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
CaM-K1delta, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzyme in Escherichia coli strain JM109, transient expression of HA-tagged CaM-K1delta 1-296 Ca2+/calmodulin-independent mutant in HeLa and COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information CaM-K1delta 1-296 truncation mutant is catalytically active and Ca2+/calmodulin-independent, lacks the putative autoinhibitory domain and the Ca2+/calmodulin binding region, the truncation mutant is fully activated by CaM-KKalpha Homo sapiens
T180A site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme, the mutant is phosphorylated and activated by CaM-KKalpha Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
STO-609 the CaM-KKalpha inhibitor STO-609 inhibits activity of the CaM-K1delta truncation mutant 1-296 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolutely dependent on Homo sapiens
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens components of the Ca2+/calmodulin-dependent protein kinase cascade, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IU85 isozyme CaM-K1delta
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha, mechanism, CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant CaM-K1delta from Escherichia coli by glutathione affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
additional information CaM-K1delta tissue expression pattern, no expression in intestine, kidney, placenta, heart, and lung Homo sapiens
-
ovary
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-
prostate gland low expression level of CaM-K1delta Homo sapiens
-
skeletal muscle low expression level of CaM-K1delta Homo sapiens
-
spleen
-
 Homo sapiens
-
testis high expression level of CaM-K1delta Homo sapiens
-
thymus low expression level of CaM-K1delta Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.035
-
 purified recombinant CaM-K1delta Homo sapiens
0.98
-
 purified recombinant CaM-K1delta phosphorylated by CaM-KKalpha Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + syntide-2
-
 Homo sapiens ADP + phosphorylated syntide-2
-
 ?
additional information components of the Ca2+/calmodulin-dependent protein kinase cascade, overview Homo sapiens ?
-
 ?
additional information CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CaM-K1delta
-
 Homo sapiens
CaM-KKbeta3
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens