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(Z)-hexadec-9-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
(Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacetyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
-
?
dodec-5-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
?
malonyl-ACP + lauroyl-ACP
?
-
-
-
-
?
malonyl-CoA + lauroyl-CoA
?
-
-
-
-
?
malonyl-CoA + palmitoyl-[acyl-carrier protein]
?
-
-
-
-
?
malonyl-phosphopantetheine + lauroyl-CoA
?
-
-
-
-
?
malonyl-phosphopantetheine-14-mer + lauroyl-CoA
?
-
-
-
-
?
malonyl-phosphopantetheine-16-mer + lauroyl-CoA
?
-
-
-
-
?
malonyl-phosphopantetheine-8-mer + lauroyl-CoA
?
-
-
-
-
?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
palmitoleoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-vaccenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
-
?
tetradec-7-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
?
additional information
?
-
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
-
?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
-
-
-
?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
-
?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
? + CO2 + [acyl-carrier protein]
-
-
-
-
?
additional information
?
-
-
KASII elongates 16:0-acyl carrier protein to 18:0-acyl carrier protein in the plastid, where it competes with three other enzymes at the first major branch point in fatty acid biosynthesis
-
-
?
additional information
?
-
KAS II catalyzes the elongation of 16:0 fatty acid-[acyl-carrier-protein] to 18:0 fatty acid-[acyl-carrier-protein] in plastids
-
-
?
additional information
?
-
-
KAS II catalyzes the elongation of 16:0 fatty acid-[acyl-carrier-protein] to 18:0 fatty acid-[acyl-carrier-protein] in plastids
-
-
?
additional information
?
-
-
the enzyme is involved in elongation of palmitoyl-[acyl-carrier-protein] to stearoyl-[acyl-carrier-protein]
-
-
?
additional information
?
-
-
FabF1 is able to catalyze all of the elongation reactions required in the synthesis of saturated fatty acids. The single 3-ketoacyl-[acyl-carrier-protein] synthase FabF of this bacterium performs the elongation functions required in both branches of the fatty acid synthetic pathway. The enzyme can both elongate palmitoleoyl-[acyl-carrier-protein] to cis-vaccenoyl-[acyl-carrier-protein] and elongate the cis double bond containing the product of FabA
-
-
?
additional information
?
-
-
of the enzymes encoded by fabF homologues designated as CAC3573, CAC2008 and CAA0093, only the first of these genes, fabF1, functions in fatty acid synthesis and can functionally replace Escherichia coli FabF in vivo, overview
-
-
?
additional information
?
-
-
altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants. F-ACP is a modification of ACP that is detected when beta-ketoacyl-ACP synthase II activity is impaired
-
-
?
additional information
?
-
beta-ketoacyl-acyl carrier protein synthase II is centrally involved in the temperature regulation of the fatty acid composition of the membrane phospholipid of Escherichia coli. The genetic locus of the Cvc lesion is designated fabF
-
-
?
additional information
?
-
-
beta-ketoacyl-acyl carrier protein synthase II is centrally involved in the temperature regulation of the fatty acid composition of the membrane phospholipid of Escherichia coli. The genetic locus of the Cvc lesion is designated fabF
-
-
?
additional information
?
-
proposed role of the enzyme in the modulation of fatty acid synthesis by temperature
-
-
?
additional information
?
-
the enzyme carries out the elongation step in fatty acid synthesis
-
-
?
additional information
?
-
-
the enzyme carries out the elongation step in fatty acid synthesis
-
-
?
additional information
?
-
-
PfFabB/F does not elongate C16DELTA9-[acyl-carrier-protein], substrate specificity, overview
-
-
?
additional information
?
-
-
enzyme forms FabF1 and FabB are functionally overlapping but not identical. FabF1 is largely a functional replacement for FabB but differs from the latter in that it does not have a severe detrimental impact on physiology
-
-
-
additional information
?
-
-
enzyme forms FabF1 and FabB are functionally overlapping but not identical. FabF1 is largely a functional replacement for FabB but differs from the latter in that it does not have a severe detrimental impact on physiology
-
-
-
additional information
?
-
-
the enzyme plays a key role in synthesis of C18 fatty acids
-
-
?
additional information
?
-
-
enzyme is inactive with stearoyl-[acyl-carrier-protein]
-
-
?
additional information
?
-
-
FabF produces C14 long-chain beta-ketoacyl-ACP
-
-
?
additional information
?
-
-
analysis of interaction between FabF and the acyl-carrier protein
-
-
?
additional information
?
-
Q7CJ22
enzyme substrate specificity, overview
-
-
?
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(2E)-3-(3-chlorophenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]prop-2-enamide
ME0619
1,3-dichloro-7-(2,4-dihydroxy-6-methylphenyl)-2,4,6,9-tetrahydroxy-12,12-dimethyltetracen-5(12H)-one
-
i.e. fasamycin B
1-chloro-7-(2,4-dihydroxy-6-methylphenyl)-2,4,6,9-tetrahydroxy-12,12-dimethyltetracen-5(12H)-one
-
i.e. fasamycin A
2-[(2R)-4-ethyl-3-hydroxy-2-[(1E)-2-methylbuta-1,3-dien-1-yl]-5-oxo-2,5-dihydrothiophen-2-yl]acetamide
-
-
3,6-dichloro-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]-1-benzothiophene-2-carboxamide
ME0640
3,6-dichloro-N-[5-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]pyridin-2-yl]-1-benzothiophene-2-carboxamide
ME0518
3-(benzoylamino)-2-hydroxybenzoic acid
binds outside the active site of the enzyme, binding structure with wild-type and C164Q mutant enzymes, overview. Access to the depths of the active site of the PaFabF apoenzyme is restricted by the conformations of Phe230 and Phe400. 3-(benzoylamino)-2-hydroxybenzoic acid/Mg2+ ion pair selectively binds into and perhaps contributes to the formation of a stable binding site on the surface of the enzyme distant from the active site, from which it is likely to be occluded by steric hindrance
3-benzamido-2-hydroxybenzoic acid
-
5-chloro-2-(2,4-dichlorophenoxy)phenol
Acyl carrier protein
0.0017 mM, 50% inhibition of myristic acid transfer from myristoyl-[acyl-carrier protein] to wild-type enzyme
arsenite
-
1 mM, 42% inhibition
casticin
i.e. 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-3,6,7-trimethoxy-4H-chromen-4-one
dihydroplatensimycin
IC50: 97 nM
iodoacetamide
prior incubation of the enzymes with fatty acyl thioesters prevents inhibition
NEM
-
5 mM, complete inhibition
PCMB
-
1 mM, complete inhibition
5-chloro-2-(2,4-dichlorophenoxy)phenol
-
Triclosan
5-chloro-2-(2,4-dichlorophenoxy)phenol
-
Triclosan
cerulenin
-
0.1 mM, 50% inhibition
cerulenin
-
originally isolated from the fungus Cephalosporium caerulensand, an irreversible inhibitor of FabF
cerulenin
binding structure with mutant C163Q
cerulenin
-
originally isolated from the fungus Cephalosporium caerulensand, an irreversible inhibitor of FabF
cerulenin
-
0.05 mM, 50% inhibition
cerulenin
-
originally isolated from the fungus Cephalosporium caerulensand, an irreversible inhibitor of FabF
cerulenin
-
blocking active site cysteine
fasamycin A
-
-
fasamycin B
-
-
phomallenic acid C
-
-
platencin
-
exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, FabF IC50: 113 nM, overview
platencin A1
-
also active against FabH, EC 2.3.1.180
platencin A1
-
also active against FabH, EC 2.3.1.180
platencin A1
-
also active against FabH, EC 2.3.1.180
platensimycin
-
platensimycin
from Streptomyces platensis, IC50: 160 nM, anti-bacterial effect is exerted through the selective targeting of beta-ketoacyl-[acyl-carrier-protein] synthase I/II, FabF/B, in the synthetic pathway of fatty acids, platensimycin interacts specifically with the acyl-enzyme intermediate of the target protein, a specific conformational change that occurs on acylation must take place before the inhibitor can bind, overview, platensimycin shows no cross-resistance to other key antibiotic-resistant strains, binding structure with mutant C163Q
platensimycin
a natural product inhibitor
platensimycin
-
from Streptomyces platensis, IC50: 48 nM, anti-bacterial effect is exerted through the selective targeting of beta-ketoacyl-[acyl-carrier-protein] synthase I/II, FabF/B, in the synthetic pathway of fatty acids, platensimycin interacts specifically with the acyl-enzyme intermediate of the target protein, a specific conformational change that occurs on acylation must take place before the inhibitor can bind, overview, platensimycin shows no cross-resistance to other key antibiotic-resistant strains
platensimycin
-
from a strain of Streptomyces platensis MA7339, specifically targets FabF, IC50: 290 nM, exhibits Gram-positive antibacterial activity
thiolactomycin
-
-
thiolactomycin
binding structure with mutant C163Q, IC50: 1.1 mM
T3010
-
-
additional information
not inhibited by triclosan
-
additional information
-
inhibitors of bacterial FASII can act as potential antibacterial agents, structure-activity relationships of the inhibitors that mainly target beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP reductase, beta-hydroxyacyl-ACP dehydratase, and enoyl-ACP reductase, overview. Screening of phomalenic acids for enzyme inhibition
-
additional information
-
inhibitors of bacterial FASII can act as potential antibacterial agents, structure-activity relationships of the inhibitors that mainly target beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP reductase, beta-hydroxyacyl-ACP dehydratase, and enoyl-ACP reductase, overview. Screening of phomalenic acids for enzyme inhibition
-
additional information
-
in vivo inhibition assays
-
additional information
-
inhibitors of bacterial FASII can act as potential antibacterial agents, structure-activity relationships of the inhibitors that mainly target beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP reductase, beta-hydroxyacyl-ACP dehydratase, and enoyl-ACP reductase, overview. Screening of phomalenic acids for enzyme inhibition
-
additional information
-
not inhibited by platensimycin
-
additional information
-
not inhibited by platensimycin
-
additional information
Q7CJ22
inhibitor interaction with the active site, structure analysis, docking study, overview
-
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evolution
the enzyme protein contains conserved domains of KAS-I and II, elongating condensing enzymes, condensing enzymes superfamily, and 3-oxoacyl-[ACP] synthase II. Comparisons of Elaeis oleifera and Elaeis guineensis enzymes, overview
evolution
the enzyme protein contains conserved domains of KAS-I and II, elongating condensing enzymes, condensing enzymes superfamily, and 3-oxoacyl-[ACP] synthase II. Comparisons of Elaeis oleifera and Elaeis guineensis enzymes, overview
malfunction
-
endogenous H2O2 (mainly produced by pyruvate oxidase) inhibits FabF activity by specifically oxidizing its active site cysteine-thiol residue
malfunction
-
KASII inhibition does not significantly influence the wax ester and triacylglycerol levels in the plant, but the the C16/C18 ratio is significantly increased in total lipid extracts of leaf tissue when KASIIRNAi-3 is expressed with or without wax ester biosynthesis gene
metabolism
KAS-II enzyme catalyzes the elongation of C16:0-ACP to C18:0-ACP in plastidial fatty acid biosynthesis pathway
metabolism
rate-limiting KAS-II enzyme catalyzes the elongation of C16:0-ACP to C18:0-ACP in plastidial fatty acid biosynthesis pathway
metabolism
Q7CJ22
the beta-ketoacyl-acyl carrier protein (ACP) synthases, FabB, FabF, and FabH, catalyse the Claisen condensation of fatty acyl-thioesters and malonyl-ACP to form a 3-oxoacyl-ACP intermediate elongated by two carbon atoms. The initial cycle of elongation is catalysed by FabH, involving condensation of malonyl-ACP and acetyl-CoA, while subsequent cycles of elongation are performed by FabB or FabF
metabolism
the enzyme is involved in fatty-acid biosynthesis
metabolism
enzyme overexpression increases pinocembrin production in recombinant Escherichia coli
physiological function
KAS2 is necessary for embryo development
physiological function
-
encoded in the gene cluster required for biosynthesis of the calcium dependent antibiotics
physiological function
-
fatty acid synthesis type II system enzymes are essential for bacterial membrane lipid biosynthesis
physiological function
-
fatty acid synthesis type II system enzymes are essential for bacterial membrane lipid biosynthesis
physiological function
-
fatty acid synthesis type II system enzymes are essential for bacterial membrane lipid biosynthesis. All FASII systems have initiation and elongation condensing enzymes which can catalyze the Claisen condensation of an acyl donor and malonyl-ACP to form a beta-ketoacyl-ACP
physiological function
the enzyme is involved in fatty-acid biosynthesis
physiological function
the level of the C16:0 in palm oil is dependent on the palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, and beta-ketoacyl-[ACP] synthase-II, EC 2.3.1.179, enzymes efficiency. Palm oil obtained from Elaeis guineensis contains about 44% palmitic acid
physiological function
the level of the C16:0 in palm oil is dependent on the palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, and beta-ketoacyl-[ACP] synthase-II, EC 2.3.1.179, enzymes efficiency. Palm oil obtained from Elaeis oleifera contains about 25% palmitic acid
additional information
active site structure of wild-type and mutant enzymes, ligand binding structures, overview
additional information
Q7CJ22
enzyme structure and active site architecture, comparison with FabH, EC 2.3.1.180. Substrate binding is controlled by residue Phe401
additional information
enzyme three-dimensional structure determination, molecular dynamics simulation, and comparison with the enzyme structure of Elaeis guineensis KASII, as well as with Streptococcus pneumonia KASII and Brucella melitensis KASII structures, molecular homology modelling, overview. Active sites of EoKASII are Cys316, His453, and His489
additional information
-
enzyme three-dimensional structure determination, molecular dynamics simulation, and comparison with the enzyme structure of Elaeis guineensis KASII, as well as with Streptococcus pneumonia KASII and Brucella melitensis KASII structures, molecular homology modelling, overview. Active sites of EoKASII are Cys316, His453, and His489
additional information
enzyme three-dimensional structure determination, molecular dynamics simulation, and comparison with the enzyme structure of Elaeis oleifera KASII, as well as with Streptococcus pneumonia KASII and Brucella melitensis KASII structures, molecular homology modelling, overview. Active site residues of EgKASII are Cys316, His456, and His492
additional information
-
enzyme three-dimensional structure determination, molecular dynamics simulation, and comparison with the enzyme structure of Elaeis oleifera KASII, as well as with Streptococcus pneumonia KASII and Brucella melitensis KASII structures, molecular homology modelling, overview. Active site residues of EgKASII are Cys316, His456, and His492
additional information
the enzyme has two active site His residues
additional information
-
the enzyme has two active site His residues
additional information
the enzyme has two active site His residues
additional information
-
the enzyme has two active site His residues
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L337F
the point mutation, mutant fab1-1, causes a partially deficient KAS2 activity
E122K
the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides
G200S
the mutant is resistant towards inhibition by acylated sulfonamides
E122K
-
the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides
-
G200S
-
the mutant is resistant towards inhibition by acylated sulfonamides
-
C163Q
site-directed mutagenesis, interaction with platensimycin compared to the interaction with the wild-type enzyme
R206G
-
R206 impairs the binding of CoA
C164Q
site-directed mutagenesis, a mutant in which the binding site is altered to resemble the substrate-bound state
C164A
-
site-directed mutagenesis, inactive mutant
C164A/H337A
-
site-directed mutagenesis, inactive mutant
C164A/K332A
-
site-directed mutagenesis, inactive mutant
E346A
-
site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme
E383A
crystal structure determination and comparison to the wild-type enzyme, the mutation E383A appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form
E396A
-
site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity
H303A
-
site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme
H337A
-
site-directed mutagenesis, inactive mutant
K332A
-
site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity
F107I
-
less efficient than wild type protein
F107L
-
less efficient than wild type protein
F107S
-
suggested that the Ser residue has less significant effects than the Ile and Leu mutations
additional information
-
construction of KASII knockout mutants by T-DNA disruption, knockout alleles fab1-1 and fab1-2, strong seed-specific hairpin-RNAi reductions in FAB1 expression resulted in abortion of about 1/4 of the embryos in an apparent phenocopy of fab1-2 homozygosity, in less severe FAB1 hairpin-RNAi individuals, embryos developed normally and exhibited a 1:2:1 segregation ratio for palmitate accumulation, thus, early embryo development appears sensitive to elevated 16:0, whereas at later stages, up to 53% of 16:0, i.e. a 7-fold increase over wild-type levels, is tolerated, Fab1-1 mutant plants show about 60% of wild-type enzyme activity and about 17% palm-like oil accumulation compared to the wild-type plants, phenotypes, overview
additional information
construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview
additional information
-
construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview
additional information
transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs
additional information
-
expression of Clostridium acetobutylicium FabF1 restores thermal control of fatty acid composition to an Escherichia coli FabF null mutant strain. FabF1 expression leads a modest conversion of cis-3-decenoyl-[acyl-carrier-protein] to trans-2-cis-5-dodecadienoyl-[acyl carrier-protein]. An Escherichia coli fabF- strain in which Clostridium acetobutylicium FabF1 is expressed from the lac promoter of a low copy number vector closely mimicks the changes in fatty acid composition seen in wild type Escherichia coli strains upon changes in growth temperature. Expression of Clostridium acetobutylicium FabF1 restores cis-vaccenate synthesis at all temperatures, but is much more effective at 30°C than at 37°C or 42°C
additional information
enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis
additional information
-
enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis
additional information
-
transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs
additional information
-
in vitro complementation of the enzyme-deficient Escherichia coli CY244 strain, shows that Plasmodium falciparum FabB/F functions like Escherichia coli FabF as the growth of the mutant cells can be rescued only in the presence of oleic acid
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AtKAS2, DNA and amino acid sequence determination and analysis, sequence comparisons, expression pattern, the promoter is active in various tissues, also in the embryo
chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells
construction of plasmids pXspFabF(M1) and pXspFabF(M2). The resulting plasmids are transformed into Escherichia coli XL1-Blue competent cells. Subsequently, pXSpFabF(M1) and pXspFabF(M2) isolated from Escherichia coli XL1-Blue cells are transformed into the expression strain Escherichia coli BL21 (DE3)
determined with the multiple isomorphous replacement method and refined at 2.4 A resolution. Hanging drop vapor diffusion method at room temperature, using 27% PEG 8000 as precipitant, buffered at pH 7.5 with 0.1 M HEPES. The crystals grow to a size of 0.5 * 0.3 * 0.2 mm3 within 3 days. The lifetime of these crystals is very limited, they will dissolve within 10 days of their appearance. Addition of 0.1% mercaptoethanol to the reservoir solution significantly increases the life time of the crystals. Space group: P3(1)21 with cell dimensions a = 76.4 A, c = 146.8 A, gamma = 120°
expressed in Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3)
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli cells lacking FabF
expression in Escherichia coli
expression of wild-type and mutant His-tagged FabF in Escherichia coli strain BL21(DE3)
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gene fabF, recombinant expression of N-terminally His6-tagged enzyme with a TEV protease cleavage site in Escherichia coli strain BL21(DE3) pLysS
Q7CJ22
gene fabF, recombinant wild-type and mutant N-terminally His6-tagged enzyme expressions from vector pNIC28-Bsa4 including a TEV cleavage site in Escherichia coli strain BL21(DE3)pLysS
gene fabF, subcloning in Escherichia coli strain XL1-Blue, expression in Escherichia coli strain BL21(DE3)
gene fabF1, expression in Escherichia coli strain BL21(DE3), and in strain K1060, a strain that carries an unconditional fabB allele, and in Escherichia coli strain CY242, which carries the same fabB(Ts) allele as strain CY244, but fabF1 fails to complement growth of the temperature sensitive fabB mutant strain CY242 at 42°C
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gene KAS-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, cloning and expression in Escherichia coli strain DH5alpha
gene KAS2, bothKASII genes are simultaneously inhibited via three different RNAi constructs, quantitative real-time PCR enzyme expression analysis
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gene KAS2, transient expression in Nicotiana bethaminana, in which both KASII genes are simultaneously inhibited via three different RNAi constructs, using Agrobacterium tumefaciens transfection method
gene KASII, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, cloning and expression in Escherichia coli strain DH5alpha
gene PffabB/Fm, FabB/F is encoded at locus MAL6P1.165, cloning and expression, expression in and complementation of Escherichia coli strain CY244, which is deficient in FabB and FabF, the latter by mutations S220N and G262M
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His-tagged protein expressed in Escherichia coli BL21(DE3)
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His-tagged protein expressed in Escherichia coli is insoluble and not functional, GFP fusion protein expressed in Arabidopsis thaliana, Arabidopsis plants expressing GFP fusions have elevated levels of arachidic acid (C20:0) and erucic acid (C22:1) at the expense of stearic acid (C18:0) and oleic acid (C18:1)
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His-tagged protein expressed in Escherichia coli pLysS
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Lactococcus lactis FabF can functionally replace both the FabB (EC 2.3.1.41) and FabF (2.3.1.179) proteins of Escherichia coli and the FabH protein of Lactococcus lactis
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overexpressed in Enterococcus faecalis
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chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells
-
chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells
-
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Platensimycin is a selective FabF inhibitor with potent antibiotic properties
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Staphylococcus aureus, Escherichia coli (P0AAI5)
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Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil
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Arabidopsis thaliana, Arabidopsis sp.
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Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties
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Staphylococcus aureus
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Surface-entropy reduction approaches to manipulate crystal forms of beta-ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae
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Streptococcus pneumoniae (Q9FBC2), Streptococcus pneumoniae
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The Lactococcus lactis FabF fatty acid synthetic enzyme can functionally replace both the FabB and FabF proteins of Escherichia coli and the FabH protein of Lactococcus lactis
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Surface-entropy reduction approaches to manipulate crystal forms of beta-ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae
Acta Crystallogr. Sect. D
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Streptococcus pneumoniae (Q9FBC2), Streptococcus pneumoniae
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Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis
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Clostridium acetobutylicum
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Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana
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Arabidopsis thaliana (Q9C9P4), Arabidopsis thaliana
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Sharma, S.; Sharma, S.K.; Surolia, N.; Surolia, A.
beta-Ketoacyl-ACP synthase I/II from Plasmodium falciparum (PfFabB/F) - is it B or F?
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Plasmodium falciparum
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Endogenous H2O2 produced by Streptococcus pneumoniae controls FabF activity
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Streptococcus pneumoniae
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Substrate recognition by beta-ketoacyl-ACP synthases
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Escherichia coli
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Active site modification of the beta-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of the CDA lipopeptides
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Streptomyces coelicolor
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Environmental DNA-encoded antibiotics fasamycins A and B inhibit FabF in type II fatty acid biosynthesis
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Enterococcus faecalis, Staphylococcus aureus, Enterococcus faecalis OG1RF
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Biochemical studies and purification of oil palm (Elaeis guineensis Jacq.) beta-ketoacyl-acyl-carrier-protein (ACP) synthase (KAS) II enzyme
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Elaeis guineensis, Elaeis guineensis Jacq.
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Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas
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Jatropha curcas (Q000L2), Jatropha curcas
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Structures of Pseudomonas aeruginosa beta-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crysta
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Pseudomonas aeruginosa (G3XDA2)
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Wang, E.; Chinni, S.; Bhore, S.J.
Three-dimensional (3D) structure prediction of the American and African oil-palms beta-ketoacyl-[ACP] synthase-II protein by comparative modelling
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Elaeis oleifera (C3W5I8), Elaeis oleifera, Elaeis guineensis (Q9M604), Elaeis guineensis
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Wang, Y.; Ma, S.
Recent advances in inhibitors of bacterial fatty acid synthesis type II (FASII) system enzymes as potential antibacterial agents
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Enterococcus faecalis, Haemophilus influenzae, Staphylococcus aureus
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Elaeis oleifera (C3W5I8), Elaeis oleifera, Elaeis guineensis (Q9M604), Elaeis guineensis
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Nicotiana benthamiana, Arabidopsis thaliana (Q9C9P4)
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Nanson, J.D.; Himiari, Z.; Swarbrick, C.M.; Forwood, J.K.
Structural characterisation of the beta-ketoacyl-acyl carrier protein synthases, FabF and FabH, of Yersinia pestis
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Yersinia pestis (Q7CJ22)
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N-acylated derivatives of sulfamethoxazole block chlamydia fatty acid synthesis and interact with FabF
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Chlamydia trachomatis (A0A0H3MGB3), Chlamydia trachomatis 434/Bu (A0A0H3MGB3)
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Morvan, C.; Halpern, D.; Kenanian, G.; Pathania, A.; Anba-Mondoloni, J.; Lamberet, G.; Gruss, A.; Gloux, K.
The Staphylococcus aureus FASII bypass escape route from FASII inhibitors
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Staphylococcus aureus
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Sabbagh, G.; Berakdar, N.
Molecular docking study of flavonoid compounds as inhibitors of beta-ketoacyl acyl carrier protein synthase II (KAS II) of Pseudomonas aeruginosa
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Shewanella oneidensis, Shewanella oneidensis ATCC 700550
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Escherichia coli (P0AAI5)
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Crystal structure determination of a chimeric FabF by XRD
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Streptomyces albus, Streptomyces platensis, Streptomyces albus J1074
-
brenda