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Literature summary for 2.3.1.179 extracted from

  • Li, K.; Li, L.; Xu, Y.
    Crystal structure determination of a chimeric FabF by XRD (2017), Nucl. Sci. Tech., 28, 123 .
No PubMed abstract available
Search for more literature for 2.3.1.179

Cloned(Commentary)

Cloned (Comment) Organism
chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells Streptomyces albus
chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells Streptomyces platensis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using either 0.1 M sodium chloride, 0.1 M bicine pH 9.0, 20% (w/v) PEG MME 550 or 0.1 M HEPES pH 7.5, 12% (w/v) PEG 3350 or 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate Streptomyces albus
sitting drop vapor diffusion method, using either 0.1 M sodium chloride, 0.1 M bicine pH 9.0, 20% (w/v) PEG MME 550 or 0.1 M HEPES pH 7.5, 12% (w/v) PEG 3350 or 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate Streptomyces platensis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by platensimycin Streptomyces albus
additional information not inhibited by platensimycin Streptomyces platensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces albus
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces platensis
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces albus J1074
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces albus
-
-
-
Streptomyces albus J1074
-
-
-
Streptomyces platensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration Streptomyces albus
Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration Streptomyces platensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces albus a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces platensis a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces albus J1074 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, chimeric protein js200-FabF, SDS-PAGE Streptomyces albus
? x * 50000, chimeric protein js200-FabF, SDS-PAGE Streptomyces platensis

Synonyms

Synonyms Comment Organism
beta-ketoacyl-acyl-carrier-protein synthase II
-
 Streptomyces albus
beta-ketoacyl-acyl-carrier-protein synthase II
-
 Streptomyces platensis
FabF
-
 Streptomyces albus
FabF
-
 Streptomyces platensis
KAS II
-
 Streptomyces albus
KAS II
-
 Streptomyces platensis