EC Number   |
|---|
    2.3.1.179 | - |
| 2.3.1.179 | AtKAS2, DNA and amino acid sequence determination and analysis, sequence comparisons, expression pattern, the promoter is active in various tissues, also in the embryo |
| 2.3.1.179 | chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells |
| 2.3.1.179 | construction of plasmids pXspFabF(M1) and pXspFabF(M2). The resulting plasmids are transformed into Escherichia coli XL1-Blue competent cells. Subsequently, pXSpFabF(M1) and pXspFabF(M2) isolated from Escherichia coli XL1-Blue cells are transformed into the expression strain Escherichia coli BL21 (DE3) |
| 2.3.1.179 | determined with the multiple isomorphous replacement method and refined at 2.4 A resolution. Hanging drop vapor diffusion method at room temperature, using 27% PEG 8000 as precipitant, buffered at pH 7.5 with 0.1 M HEPES. The crystals grow to a size of 0.5 * 0.3 * 0.2 mm3 within 3 days. The lifetime of these crystals is very limited, they will dissolve within 10 days of their appearance. Addition of 0.1% mercaptoethanol to the reservoir solution significantly increases the life time of the crystals. Space group: P3(1)21 with cell dimensions a = 76.4 A, c = 146.8 A, gamma = 120° |
| 2.3.1.179 | expressed in Arabidopsis thaliana |
| 2.3.1.179 | expressed in Escherichia coli BL21(DE3) |
| 2.3.1.179 | expressed in Escherichia coli BL21(DE3) cells |
| 2.3.1.179 | expressed in Escherichia coli cells lacking FabF |
| 2.3.1.179 | expression in Escherichia coli |
