EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.179 | C163Q |
site-directed mutagenesis, interaction with platensimycin compared to the interaction with the wild-type enzyme |
676148 |
| 2.3.1.179 | C164A |
site-directed mutagenesis, inactive mutant |
674581 |
| 2.3.1.179 | C164A/H337A |
site-directed mutagenesis, inactive mutant |
674581 |
| 2.3.1.179 | C164A/K332A |
site-directed mutagenesis, inactive mutant |
674581 |
| 2.3.1.179 | C164Q |
site-directed mutagenesis, a mutant in which the binding site is altered to resemble the substrate-bound state |
735397 |
| 2.3.1.179 | E122K |
the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides |
-, 755804 |
| 2.3.1.179 | E346A |
site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme |
674581 |
| 2.3.1.179 | E383A |
crystal structure determination and comparison to the wild-type enzyme, the mutation E383A appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form |
701462 |
| 2.3.1.179 | E396A |
site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity |
674581 |
| 2.3.1.179 | F107I |
less efficient than wild type protein |
719275 |
