Literature summary for 2.3.1.179 extracted from

Mojica, S.A.; Salin, O.; Bastidas, R.J.; Sunduru, N.; Hedenstroem, M.; Andersson, C.D.; Nunez-Otero, C.; Engstroem, P.; Valdivia, R.H.; Elofsson, M.; Gylfe, A.
N-acylated derivatives of sulfamethoxazole block chlamydia fatty acid synthesis and interact with FabF (2017), Antimicrob. Agents Chemother., 61, e00716-17 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli cells lacking FabF	Chlamydia trachomatis

Protein Variants

Protein Variants	Comment	Organism
E122K	the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides	Chlamydia trachomatis
G200S	the mutant is resistant towards inhibition by acylated sulfonamides	Chlamydia trachomatis

General Stability

General Stability	Organism
acylated sulfonamides ME0640 (0.5 mM), ME0518 (1 mM) and ME0619 (4 mM) protect the recombinant enzyme from proteolysis	Chlamydia trachomatis

Inhibitors

Inhibitors	Comment	Organism	Structure
(2E)-3-(3-chlorophenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]prop-2-enamide	ME0619	Chlamydia trachomatis
3,6-dichloro-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]-1-benzothiophene-2-carboxamide	ME0640	Chlamydia trachomatis
3,6-dichloro-N-[5-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]pyridin-2-yl]-1-benzothiophene-2-carboxamide	ME0518	Chlamydia trachomatis
cerulenin	-	Chlamydia trachomatis
additional information	not inhibited by triclosan	Chlamydia trachomatis
platensimycin	-	Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Chlamydia trachomatis	-	a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Chlamydia trachomatis 434/Bu	-	a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydia trachomatis	A0A0H3MGB3	-	-
Chlamydia trachomatis 434/Bu	A0A0H3MGB3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and HiLoad GF200 gel filtration	Chlamydia trachomatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Chlamydia trachomatis	a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Chlamydia trachomatis 434/Bu	a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
3-oxoacyl-(acylcarrier protein) synthase II	-	Chlamydia trachomatis
FabF	-	Chlamydia trachomatis

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Release 2023.1 (January 2023)