Reference on EC 2.1.1.45 - thymidylate synthase and Organism(s) Escherichia coli and UniProt Accession P0A884
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AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wahba, A.J.; Friedkin, M.
The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli
J. Biol. Chem.
237
3794-3801
1962
Enterococcus faecalis, Escherichia coli, Escherichia coli B / ATCC 11303
Blakeley, R.L.
The biosythesis of thymidylic acid. IV. Further studies on thymidylate synthetase
J. Biol. Chem.
238
2113-2118
1963
Escherichia coli, Enterococcus faecalis, Homo sapiens
-
Fridland, A.; Langenbach, R.J.; Heidelberger, C.
Purification of thymidylate synthetase from Ehrlich ascites carcinoma cells
J. Biol. Chem.
246
7110-7114
1971
Bos taurus, Gallus gallus, Escherichia coli, Enterococcus faecalis, Homo sapiens, Lacticaseibacillus casei, Mus musculus
Horinishi, H.; Greenberg, D.M.
Purification and properties of thymidylate synthase from calf thymus
Biochim. Biophys. Acta
258
741-752
1972
Escherichia phage T2, Enterobacteria phage T6, Bos taurus, Gallus gallus, Escherichia coli, Enterococcus faecalis, Lacticaseibacillus casei, Mus musculus
Santi, D.V.; Sakai, T.T.
Irreversible inhibition of thymidylate synthetase by 5-formyl-2-deoxyuridylic acid
Biochem. Biophys. Res. Commun.
46
1320-1325
1972
Escherichia coli, Escherichia coli B / ATCC 11303, Lacticaseibacillus casei, Mus musculus
Galivan, J.; Maley, G.F.; Maley, F.
Purification and properties of T2 bacteriophage-induced thymidylate synthetase
Biochemistry
13
2282-2289
1974
Escherichia phage T2, Tequatrovirus T4, Enterobacteria phage T6, Bos taurus, Escherichia coli, Lacticaseibacillus casei, Mus musculus, Rattus norvegicus
Friedkin, M.; Plante, L.T.; Crawford, E.J.; Crumm, M.
Inhibition of thymidylate synthetase and dihydrofolate reductase by naturally occurring oligoglutamate derivatives of folic acid
J. Biol. Chem.
250
5614-5621
1975
Escherichia coli, Escherichia coli B / ATCC 11303
McCuen, R.W.; Sirotnak, F.M.
Thymidylate synthetase from Diplococcus pneumoniae, properties and inhibition by folate analogs
Biochim. Biophys. Acta
384
369-380
1975
Enterococcus faecium, Escherichia coli, Escherichia coli B / ATCC 11303, Gallus gallus, Lacticaseibacillus casei, Streptococcus pneumoniae
Slavik, K.; Rode, W.; Slavikova, V.
Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography
Biochemistry
15
4222-4227
1976
Bos taurus, Escherichia coli, Escherichia coli B / ATCC 11303, Mus musculus
Al Chalabi, K.; Gutteridge, W.E.
Presence and properties of thymidylate synthase in trypanosomatids
Biochim. Biophys. Acta
481
71-79
1977
Crithidia fasciculata, Strigomonas oncopelti, Escherichia coli, Rattus norvegicus, Trypanosoma brucei, Trypanosoma brucei gambiense, Trypanosoma congolense, Trypanosoma cruzi, Trypanosoma lewisi, Trypanosoma rhodesiense
Danenberg, P.V.
Thymidylate synthetase - a target enzyme in cancer chemotherapy
Biochim. Biophys. Acta
473
73-92
1977
Enterococcus faecalis, Escherichia coli, Escherichia coli B / ATCC 11303, Homo sapiens, Lacticaseibacillus casei, Mus musculus, Streptococcus pneumoniae
Dolnick, B.J.; Cheng, Y.C.
Human thymidylate synthetase. II. Derivatives of pteroylmono- and -polyglutamates as substrates and inhibitors
J. Biol. Chem.
253
3563-3567
1978
Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia coli R2, Homo sapiens, Lacticaseibacillus casei
Haertle, T.; Wohlrab, F.; Guschlbauer, W.
Thymidylate synthetase from Escherichia coli K12. Purification, and dependence of kinetic properties on sugar conformation and size of the 2 substituent
Eur. J. Biochem.
102
223-230
1979
Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia coli EM 20031 K12, Escherichia phage T2, Lacticaseibacillus casei, Streptococcus pneumoniae
Lockshin, A.; Moran, R.G.; Danenberg, P.V.
Thymidylate synthetase purified to homogeneity from human leukemic cells
Proc. Natl. Acad. Sci. USA
76
750-754
1979
Escherichia phage T2, Bos taurus, Escherichia coli, Homo sapiens, Lacticaseibacillus casei
Rode, W.; Scanlon, K.J.; Hynes, J.; Bertino, J.R.
Purification of mammalian tumor (L1210) thymidylate synthetase by affinity chromatography on stable biospecific adsorbent. Stabilization of the enzyme with neutral detergents
J. Biol. Chem.
254
11538-11543
1979
Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus
Slavik, K.; Slavikova, V.
Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography
Methods Enzymol.
66
709-723
1980
Bos taurus, Escherichia coli, Escherichia coli B / ATCC 11303, Lacticaseibacillus casei, Mus musculus
Belfort, M.; Moelleken, A.; Maley, G.F.; Maley, F.
Purification and properties of T4 phage thymidylate synthetase produced by the cloned gene in an amplification vector
J. Biol. Chem.
258
2045-2051
1983
Bacillus subtilis, Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia phage T2, Lacticaseibacillus casei, Rattus norvegicus, Tequatrovirus T4
Pattanakitsakul, S.N.; Ruenwongsa, P.
Characterization of thymidylate synthetase and dihydrofolate reductase from Plasmodium berghei
Int. J. Parasitol.
14
513-520
1984
Crithidia fasciculata, Streptococcus pneumoniae, Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Leishmania major, Plasmodium berghei
Matthews, D.A.; Villafranca, J.E.; Janson, C.A.; Smith, W.W.; Welsh, K.; Freer, S.
Stereochemical mechanism of action for thymidylate synthase based on the X-ray structure of the covalent inhibitory ternary complex with 5-fluoro-2-deoxyuridylate and 5,10-methylenetetrahydrofolate
J. Mol. Biol.
214
937-948
1990
Escherichia coli, Lacticaseibacillus casei
Galova, M.; Koptidesova, D.; Rusznakova, D.; Racay, P.; Kollarova, M.
Characteristics of NADPH-dependent thymidylate synthetase purified from Streptomyces aureofaciens
Arch. Biochem. Biophys.
296
81-87
1992
Bos taurus, Saccharomyces cerevisiae, Crithidia fasciculata, Escherichia coli, Mus musculus, Kitasatospora aureofaciens
Garcia-Fuentes, L.; Reche, P.; Lopez-Mayorga, O.; Santi, D.V.; Gonzalez-Pacanowska, D.; Baron, C.
Thermodynamic analysis of the binding of 5-fluoro-2'-deoxyuridine 5'-monophosphate to thymidylate synthase over a range of temperatures
Eur. J. Biochem.
232
641-645
1995
Escherichia coli, Lacticaseibacillus casei
Maley, F.; Pedersen-Lane, J.; Changchien, L.
Complete restoration of activity to inactive mutants of Escherichia coli thymidylate synthase: Evidence that E. coli thymidylate synthase is a half-the-sites activity enzyme
Biochemistry
34
1469-1474
1995
Escherichia coli, Lacticaseibacillus casei
Voeller, D.M.; Changchien, L.M.; Maley, G.F.; Maley, F.; Takechi, T.; Turner, R.E.; Montfort, W.R.; Allegra, C.J.; Chu, E.
Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA
Nucleic Acids Res.
23
869-875
1995
Tequatrovirus T4, Escherichia coli, Enterococcus faecium, Homo sapiens
Cho, S.W.; Lee, J.; Cho, E.H.
Effects of mutation at arginine-218 residue on the reaction of synthetic thymidylate synthase and 5-fluorouracil
Exp. Mol. Med.
28
77-83
1996
Tequatrovirus T4, Escherichia coli, Lacticaseibacillus casei, Plasmodium falciparum
-
Hekmat-Nejad, M.; Rathod, P.K.
Kinetics of Plasmodium flaciparum thymidylate synthase: interactions with high-affinity metabolites of 5-fluoroorotate and D1694
Antimicrob. Agents Chemother.
40
1628-1632
1996
Crithidia fasciculata, Escherichia coli, Homo sapiens, Leishmania major, Mus musculus, Plasmodium berghei, Plasmodium falciparum, Plasmodium yoelii
Spencer, H.T.; Villafranca, J.E.; Appleman, J.R.
Kinetic scheme for thymidylate synthase from Escherichia coli: determination from measurements of ligand binding, primary and secondary isotope effects, and pre-steady-state catalysis
Biochemistry
36
4212-4222
1997
Tequatrovirus T4, Candida albicans, Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus
Chiericatti, G.; Santi, D.V.
Aspartate 221 of thymidylate synthase is involved in folate cofactor binding and in catalysis
Biochemistry
37
9038-9042
1998
Escherichia coli, Lacticaseibacillus casei
Liang, P.H.; Anderson, K.S.
Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase
Biochemistry
37
12195-12205
1998
Escherichia coli, Leishmania major, Toxoplasma gondii
Stout, T.J.; Schellenberger, U.; Santi, D.V.; Stroud, R.M.
Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis
Biochemistry
37
14736-14747
1998
Lacticaseibacillus casei, Lactococcus lactis, Bacillus phage phi3T, Escherichia coli (P0A884), Escherichia coli, Bacillus subtilis (P0CI79), Bacillus subtilis
Tong, Y.; Liu-Chen, X.; Ercikan-Abali, E.A.; Capiaux, G.M.; Zhao, S.C.; Banerjee, D.; Bertino, J.R.
Isolation and characterization of Thymitaq (AG337) and 5-fluoro-2-deoxyuridylate-resistant mutants of human thymidylate synthase from ethyl methanesulfonate-exposed human sarcoma HT1080 cells
J. Biol. Chem.
273
11611-11618
1998
Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Cryptococcus neoformans (P0CS13), Cryptococcus neoformans B-3501A (P0CS13)
Fox, K.M.; Maley, F.; Garibian, A.; Changchien, L.M.; Van Roey, P.
Crystal structure of thymidylate synthase A from Bacillus subtilis
Protein Sci.
8
538-544
1999
Bacillus subtilis, Escherichia coli
Mahdavian, E.; Spencer, H.T.; Dunlap, R.B.
Kinetic studies on drug-resistant variants of Escherichia coli thymidylate sythase: Functional effects of amino acid sunstitutions at residue 4
Arch. Biochem. Biophys.
368
257-264
1999
Escherichia coli, Homo sapiens
Steadman, D.J.; Spencer, H.T.; Dunlap, R.B.; Berger, S.H.
Substitution at residue 214 of human thymidylate synthase alters nucleotide binding and isomerization of ligand-protein complexes
Biochemistry
38
5582-5587
1999
Escherichia coli, Homo sapiens, Lacticaseibacillus casei
Changchien, L.M.; Garibian, A.; Frasca, V.; Lobo, A.; Maley, G.F.; Maley, F.
High-Level expression of Escherichia coli and Bacillus subtilis thymidylate synthases
Protein Expr. Purif.
19
265-270
2000
Bacillus subtilis, Escherichia coli
Fantz, C.; Shaw, D.; Jennings, W.; Forsthoefel, A.; Kitchens, M.; Phan, J.; Minor, W.; Lebioda, L.; Berger, F.G.; Spencer, H.T.
Drug-resistant variants of Escherichia coli thymidylate synthase: effects of substitutions at Pro-254
Mol. Pharmacol.
57
359-366
2000
Homo sapiens, Mus musculus, Escherichia coli (P0A884), Escherichia coli
Phan, J.; Mahdavian, E.; Nivens, M.C.; Minor, W.; Berger, S.; Spencer, H.T.; Dunlap, R.B.; Lebioda, L.
Catalytic cysteine of thymidylate synthase is activated upon substrate binding
Biochemistry
39
6969-6978
2000
Homo sapiens, Lacticaseibacillus casei, Escherichia coli (P0A884), Escherichia coli
Golos, B.; Dzik, J.M.; Kazimierczuk, Z.; Ciesla, J.; Zielinski, Z.; Jankowska, J.; Kraszewski, A.; Stawinski, J.; Rode, W.; Shugar, D.
Interaction of thymidylate synthase with the 5'-thiophosphates, 5'-dithiophosphates, 5'-H-phosphonates and 5'-S-thiosulfates of 2'-deoxyuridine, thymidine and 5-fluoro-2'-deoxyuridine
Biol. Chem.
382
1439-1445
2001
Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus, Pneumocystis carinii, Rattus norvegicus, Trichinella pseudospiralis, Trichinella spiralis
Saxl, R.L.; Changchien, L.M.; Hardy, L.W.; Maley, F.
Parameters affecting the restoration of activity to inactive mutants of thymidylate synthase via subunit exchange: Further evidence that thymidylate synthase Is a half-of-the-sites activity enzyme
Biochemistry
40
5275-5282
2001
Escherichia coli, Lacticaseibacillus casei, Pneumocystis carinii
Fritz, T.A.; Liu, L.; Finer-Moore, J.S.; Stroud, R.M.
Tryptophan 80 and leucine 143 are critical for the hydride transfer step of thymidylate synthase by controlling active site access
Biochemistry
41
7021-7029
2002
Escherichia coli (P0A884), Escherichia coli
Gangjee, A.; Li, W.; Yang, J.; Kisliuk, R.L.
Design, synthesis, and biological evaluation of classical and nonclassical 2-amino-4-oxo-5-substituted-6-methylpyrrolo[3,2-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors
J. Med. Chem.
51
68-76
2008
Escherichia coli, Homo sapiens, Toxoplasma gondii
Arvizu-Flores, A.A.; Sugich-Miranda, R.; Arreola, R.; Garcia-Orozco, K.D.; Velazquez-Contreras, E.F.; Montfort, W.R.; Maley, F.; Sotelo-Mundo, R.R.
Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: calorimetric and crystallographic analysis of the K48Q mutant
Int. J. Biochem. Cell Biol.
40
2206-2217
2008
Escherichia coli (P0A884), Escherichia coli
Gangjee, A.; Qiu, Y.; Li, W.; Kisliuk, R.L.
Potent dual thymidylate synthase and dihydrofolate reductase inhibitors: classical and nonclassical 2-amino-4-oxo-5-arylthio-substituted-6-methylthieno[2,3-d]pyrimidine antifolates
J. Med. Chem.
51
5789-5797
2008
Escherichia coli, Toxoplasma gondii, Homo sapiens (P04818), Homo sapiens
Jarmula, A.; Dowiercial, A.; Rode, W.
A molecular modeling study of the interaction of 2-fluoro-substituted analogues of dUMP/FdUMP with thymidylate synthase
Bioorg. Med. Chem. Lett.
18
2701-2708
2008
Escherichia coli (P0A884)
Gangjee, A.; Li, W.; Kisliuk, R.L.; Cody, V.; Pace, J.; Piraino, J.; Makin, J.
Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents
J. Med. Chem.
52
4892-4902
2009
Escherichia coli, Homo sapiens, Toxoplasma gondii
Kanaan, N.; Marti, S.; Moliner, V.; Kohen, A.
QM/MM study of thymidylate synthase: Enzymatic motions and the temperature dependence of the rate limiting step (dagger)
J. Phys. Chem. A
113
2176-2182
2009
Escherichia coli
Arvizu-Flores, A.; Velazquez-Contreras, E.; Machi, L.; Arreola, R.; Maley, F.; Sotelo-Mundo, R.
Spectroscopic analysis of folate binding to thymidylate synthase active site
Spectrosc. Lett.
42
142-146
2009
Escherichia coli
-
Gangjee, A.; Jain, H.D.; Phan, J.; Guo, X.; Queener, S.F.; Kisliuk, R.L.
2,4-Diamino-5-methyl-6-substituted arylthio-furo[2,3-d]pyrimidines as novel classical and nonclassical antifolates as potential dual thymidylate synthase and dihydrofolate reductase inhibitors
Bioorg. Med. Chem.
18
953-961
2010
Escherichia coli, Homo sapiens, Toxoplasma gondii
Islam, Z.; Strutzenberg, T.S.; Ghosh, A.K.; Kohen, A.
Activation of two sequential H-transfers in the thymidylate synthase catalyzed reaction
ACS Catal.
5
6061-6068
2015
Escherichia coli (P0A884), Escherichia coli
Swiderek, K.; Kohen, A.; Moliner, V.
The influence of active site conformations on the hydride transfer step of the thymidylate synthase reaction mechanism
Phys. Chem. Chem. Phys.
17
30793-30804
2015
Escherichia coli (P0A884)
Sapienza, P.J.; Popov, K.I.; Mowrey, D.D.; Falk, B.T.; Dokholyan, N.V.; Lee, A.L.
Inter-active site communication mediated by the dimer interface beta-sheet in the half-the-sites enzyme, thymidylate synthase
Biochemistry
58
3302-3313
2019
Escherichia coli (P0A884)
