Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)tetrahydropteroylglutamate | - |
Escherichia coli | |
2-amino-4-hydroxyquinazoline folic acid | - |
Enterococcus faecalis | |
2-amino-4-hydroxyquinazoline folic acid | - |
Lacticaseibacillus casei | |
2-amino-4-hydroxyquinazoline folic acid | - |
Mus musculus | |
2-amino-4-hydroxyquinazoline folic acid | - |
Streptococcus pneumoniae | |
3-beta-D-ribofuranosylmaleimide | showdomycin | Lacticaseibacillus casei | |
3-beta-D-ribofuranosylmaleimide 5'-phosphate | showdomycin 5'-phosphate | Lacticaseibacillus casei | |
5-fluoro-2'-deoxyuridylic acid | - |
Enterococcus faecalis | |
5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
5-fluorouridine | - |
Homo sapiens | |
5-Formyl-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-Formyl-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-Formyl-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-iodoacetamidomethyl-2'-deoxyuridine 5'-phosphate | - |
Mus musculus | |
5-Mercapto-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-Mercapto-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-Mercapto-2'-deoxyuridylic acid | possesses antitumor activity against Ehrlich ascites cells | Mus musculus | |
5-trifluoromethyl-2'-deoxyuridylic acid | - |
Homo sapiens | |
bromodeoxyuridine | - |
Homo sapiens | |
cytosine arabinoside | - |
Homo sapiens | |
diglutamate | - |
Escherichia coli | |
fluoroorotic acid | - |
Homo sapiens | |
glutamate | - |
Escherichia coli | |
iodoacetamide | - |
Lacticaseibacillus casei | |
Iododeoxyuridine | - |
Homo sapiens | |
N5-formyltetrahydropteroyloligoglutamate | - |
Escherichia coli | |
pteroylhexaglutamate | - |
Escherichia coli | |
pteroylhexaglutamate | - |
Lacticaseibacillus casei | |
tetrahydrohomofolate | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Streptococcus pneumoniae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Homo sapiens | - |
human | - |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lacticaseibacillus casei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
neuroblastoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
- |
Enterococcus faecalis |
0.27 | - |
- |
Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate | - |
Escherichia coli | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
(-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate | - |
Escherichia coli B / ATCC 11303 | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Enterococcus faecalis | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Enterococcus faecalis | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | - |
Enterococcus faecalis | ? | - |
? | |
additional information | - |
Streptococcus pneumoniae | ? | - |
? | |
additional information | - |
Lacticaseibacillus casei | ? | - |
? | |
additional information | (+) forms are inhibitors | Escherichia coli | ? | - |
? | |
additional information | (+) forms are inhibitors | Escherichia coli B / ATCC 11303 | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
5-Formyl-2'-deoxyuridylic acid | - |
Homo sapiens | |
0.00001 | - |
5-trifluoromethyl-2'-deoxyuridylic acid | - |
Homo sapiens | |
0.00004 | - |
5-Mercapto-2'-deoxyuridylic acid | - |
Homo sapiens |