Application | Comment | Organism |
---|---|---|
medicine | - |
Homo sapiens |
medicine | - |
Crithidia fasciculata |
medicine | target enzyme for antitumor and antibacterial agents | Plasmodium berghei |
medicine | target enzyme for malarial chemotherapy | Plasmodium berghei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Plasmodium berghei | |
KCl | - |
Plasmodium berghei | |
methotrexate | - |
Homo sapiens | |
methotrexate | - |
Plasmodium berghei | |
NaCl | - |
Plasmodium berghei | |
Urea | - |
Plasmodium berghei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0714 | - |
2'-deoxyuridylate | - |
Plasmodium berghei | |
0.3125 | - |
5,10-methylenetetrahydrofolate | - |
Plasmodium berghei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
2 * 65000, SDS-PAGE | Plasmodium berghei |
132000 | - |
gel filtration | Plasmodium berghei |
150000 | - |
gel filtration | Crithidia fasciculata |
150000 | - |
gel filtration | Plasmodium berghei |
150000 | - |
gel filtration | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Streptococcus pneumoniae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Leishmania major | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Plasmodium berghei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
human | - |
Lacticaseibacillus casei | - |
- |
- |
Leishmania major | - |
- |
- |
Plasmodium berghei | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
partially, copurified with dihydrofolate reductase | Plasmodium berghei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blast cell | from patients with acute myelocytic leukemia | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00235 | - |
- |
Plasmodium berghei |
Storage Stability | Organism |
---|---|
4°C, enzyme loses half of its activity upon storage for a few days | Plasmodium berghei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium berghei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Plasmodium berghei | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Homo sapiens |
dimer | - |
Streptococcus pneumoniae |
dimer | - |
Lacticaseibacillus casei |
dimer | - |
Leishmania major |
dimer | 2 * 65000, SDS-PAGE | Plasmodium berghei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Plasmodium berghei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | stable in this range but loses activity completely below pH 3.5 or above pH 11.5 | Plasmodium berghei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Plasmodium berghei | |
0.103 | - |
methotrexate | uncompetitive with dUMP | Plasmodium berghei |