Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | human TS mutants have potential applications in gene therapy by protecting hematopoietic progenitors from TS inhibitor toxicity, drug-resistant variants can be used to decrease the myelosuppressive side effects of TS-directed anticancer agents or to select genetically modified cells in vivo, clincal trials introduce chemoprotecting genes into hematopoietic cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant P254D cDNA cloned into SacII/XhoI sites of the HaMSV retroviral vector and transfected into the TS negative cell line RJK88.13 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
mutant P254S | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
methylenetetrahydropteroylgluamate | mutant P254S | Escherichia coli | |
0.0011 | - |
methylenetetrahydropteroylgluamate | wild-type | Escherichia coli | |
0.0012 | - |
2'-deoxyuridylate | wild-type | Escherichia coli | |
0.0012 | - |
methylenetetrahydropteroylgluamate | mutant P254G | Escherichia coli | |
0.0015 | - |
2'-deoxyuridylate | mutant P254G | Escherichia coli | |
0.0018 | - |
2'-deoxyuridylate | mutant P254F | Escherichia coli | |
0.0018 | - |
methylenetetrahydropteroylgluamate | mutant P254F | Escherichia coli | |
0.0027 | - |
2'-deoxyuridylate | mutant P254S | Escherichia coli | |
0.0034 | - |
2'-deoxyuridylate | mutant P254D | Escherichia coli | |
0.0118 | - |
methylenetetrahydropteroylgluamate | mutant P254D | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A884 | - |
- |
Homo sapiens | - |
human | - |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Pro-254 mutant enzymes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Mus musculus | - |
bone marrow | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
methylenetetrahydropteroylglutamate + dUMP | - |
Escherichia coli | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life of wild-type enzyme is 90 min, half-life of mutants P254F, P254S and P254D are approximately 50 min | Homo sapiens |