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Literature summary for 2.1.1.45 extracted from
- Aspartate 221 of thymidylate synthase is involved in folate cofactor binding and in catalysis (1998), Biochemistry, 37, 9038-9042.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Lacticaseibacillus casei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
2'-deoxyuridylate | wild-type | Lacticaseibacillus casei |  |
| 0.0057 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.0057 | - |
5-bromo-2'-deoxyuridylate | wild-type | Escherichia coli | |
| 0.0063 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.0063 | - |
2'-deoxyuridylate | mutant D221S | Escherichia coli | |
| 0.011 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.011 | - |
2'-deoxyuridylate | mutant D221E | Escherichia coli | |
| 0.015 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.015 | - |
2'-deoxyuridylate | mutant D221C | Escherichia coli | |
| 0.02 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.02 | - |
5-bromo-2'-deoxyuridylate | mutant D221A | Escherichia coli | |
| 0.025 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.025 | - |
5-bromo-2'-deoxyuridylate | mutant D221C | Escherichia coli | |
| 0.027 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.027 | - |
5-bromo-2'-deoxyuridylate | mutant D221S | Escherichia coli | |
| 0.045 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.045 | - |
5-bromo-2'-deoxyuridylate | mutant D221N | Escherichia coli | |
| 0.046 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.046 | - |
5-bromo-2'-deoxyuridylate | mutant D221E | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Lacticaseibacillus casei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant enzymes D221A, N, R, F, C, E, and S | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate | some mutants are capable of forming covalent thymidylate synthase-5-fluoro-dUMP-methylenetetrahydrofolate complex | Lacticaseibacillus casei | 7,8-dihydrofolate + ? | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Escherichia coli | ? | - |
? | |
| 5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Lacticaseibacillus casei | ? | - |
? | |
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