Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.81: flavanoid 3',5'-hydroxylase

This is an abbreviated version!
For detailed information about flavanoid 3',5'-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.81

Reaction

a 3'-hydroxyflavanone
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 3',5'-dihydroxyflavanone
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

Cyp75A, CYP75A31, CYP75A42, CYP75A47, CYP75A48, cytochrome P450-like flavonoid 3'5'-hydroxylase, DkF3'5'H, EC 1.14.13.88, F3',5'H, F3'5'H, flavonoid 3',5'-hydroxylase, flavonoid 3'5' hydroxylase, flavonoid 3'5'-hydroxylase, More, plant P450 flavonoid 3',5'-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.81 flavanoid 3',5'-hydroxylase

Cloned

Cloned on EC 1.14.14.81 - flavanoid 3',5'-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, genetic mapping, phylogenetic analysis, expression analysis and metabolic profiling, phenotypes
DNA and amino acid sequence determination and analysis, phylogenetic analysis of sequences of both enzymes indicate that F3',5'H is recruited from F3'H before the divergence of angiosperms and gymnosperms, overview
DNA and amino acid sequence determination and analysis, sequence comparison, genetic organization, and phylogenetic analysis
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme overexpression in Petunia hybrida hybrid M1 x R27, which contains a mutated endogenous F3'5'H (HF1), no obvious phenotype
-
expression in Dendrathemum grandiflora transgenic plants using the Agrobacterium tumefaciens stran ABA 4404 transfection method
-
expression in Escherichia coli JM109
expression in Petunia hybrida
expression in Rosa hybrida transgenic plants, the mutant plants show the accumulation of a high percentage of delphinidin in selected cultuvars, while the wild-type lacks delphinidin and derivatives and the activity of F3'5'H
Viola sp.
-
expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes
functional expression of the enzyme as fusion enzyme with a P450 reductase leading to biosynthesis of plant-specific di- and trihydroxylated flavonols in Escherichia coli strain BL21(DE3), feeding experiments and determination of the flavonoid spectra in different recombinant bacterial lines in vivo, overview
-
gene CYP75A31, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae microsomes using vector pYeDP60, real-time PCR expression analysis
gene F3',5'h, DNA and amino acid sequence determination and analysis, genomic structure, expression pattern analysis
gene F3',5'H1, DNA and amino acid sequence determination and analysis, phylogenetic tree, developmental expression analysis, comparison of red and white cultivar enzyme expression levels, functional expression in Petunia hybrida altering the hosts' flower color and flavonoid composition
introduction of a flavonoid 3'5' hydroxylase sequence into Lotus root cultures. Expression of the transgene is associated with increased levels of condensed tannins, no alteration in polymer hydroxylation
-
Met210 variant, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression analysis
-
one copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons, genotyping, quantitative RT-PCR expression analysis
PCR-amplification of isolated cDNA
PCR-amplification, transformation into Agrobacterium tumefaciens EHA101 by electroporation used to transform the blue-flowered Gentiana triflora x Gentiana scabra cultivar Albireo via leaf disc infection
subcloning of AK14, encoding flavonoid-3',5'-hydroxylase, into a plant expression vector and transforming it to pink tobacco (Nicotiana tabacum cv. Petit Havana SR1) and pink petunia (var. Falcon), both of which originally lack the enzyme
-
subcloning of TG1, encoding flavonoid-3',5'-hydroxylase, into a plant expression vector and transforming it to pink tobacco (Nicotiana tabacum cv. Petit Havana SR1) which originally lacks the enzyme
the gene from Campanula medium is introduced into Chrysanthemum by using binary vector pB249. Two transgenes, namely, CamF3'5'H and CtA3'5'GT, are enough to generate blue chrysanthemum: The 3',5'-diglucosylated delphinidin exhibits a blue color by intermolecular association with flavone glucosides under the weakly acidic pH conditions of general flower petals
transcription profiling of different cultivars, overview
-
Val210 variant, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression analysis
-
when sense constructs are introduced into pink flower varieties that are deficient in the enzyme, transgenic plants show flower color changes from pink to magenta along with changes ihn anthocyanin composition. Some transgenic plants show novel pigmentation patterns, e.g. a star-shaped pattern. When sense constructs are introduced into blue flower petunia varieties, the flower color of the transgenic plants changes from deep blue to pale blue or even pale pink. Pigment composition analysis of transgenic plants suggests that the F3'5'H transgene not only creates or inhibits the biosynthetic pathway to 3',5'-hydroxylated anthocyanins but switches the pathway to 3',5'-hydroxylated or 3'-hydroxylated anthocyanins
-
when VmFH1, encoding the flavonoid 3',5'-hydroxylase is expressed in transgenic petunia hybrida under the control of the cauliflower mosaic virus 35S promoter, some transgenic plants show drastic flower color alteration from red to deep red with deep purple sectors