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1.14.14.81: flavanoid 3',5'-hydroxylase

This is an abbreviated version!
For detailed information about flavanoid 3',5'-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.81

Reaction

a 3'-hydroxyflavanone
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
a 3',5'-dihydroxyflavanone
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

Cyp75A, CYP75A31, CYP75A42, CYP75A47, CYP75A48, cytochrome P450-like flavonoid 3'5'-hydroxylase, DkF3'5'H, EC 1.14.13.88, F3',5'H, F3'5'H, flavonoid 3',5'-hydroxylase, flavonoid 3'5' hydroxylase, flavonoid 3'5'-hydroxylase, More, plant P450 flavonoid 3',5'-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.81 flavanoid 3',5'-hydroxylase

Inhibitors

Inhibitors on EC 1.14.14.81 - flavanoid 3',5'-hydroxylase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-aminobenzotriazole
-
-
cytochrome c
-
0.05 mM, complete inhibition
decyl-beta-D-glucopyranoside
-
dose-dependent inhibition
diethyldithiocarbamate
-
-
Digitonin
-
dose-dependent inhibition
dodecyl maltoside
-
dose-dependent inhibition
Emulgen 911
-
dose-dependent inhibition
-
Emulgen 913
-
dose-dependent inhibition
heptyl-beta-D-glucopyranoside
-
dose-dependent inhibition
hexyl-beta-D-glucopyranoside
-
dose-dependent inhibition
Lubrol Px
-
dose-dependent inhibition
Mega-10
-
dose-dependent inhibition
Mega-8
-
dose-dependent inhibition
Mega-9
-
dose-dependent inhibition
Nonidet P40
-
dose-dependent inhibition
nonyl-beta-D-glucopyranoside
-
dose-dependent inhibition
octyl-beta-D-glucopyranoside
-
dose-dependent inhibition
RNAi
reduces transcript levels, thus affecting flower color, acylated delphinidins (dominating in the wild-type) including gentiodelphin are decreased to 12.0% while non-acylated delphinidins are increased to 66.0 and 69.7% (15.2% in wild-type) of total anthocyanins in two transformed strains (clone 1 lilac, clone 15 pale blue)
-
sodium cholate
-
dose-dependent inhibition
sodium deoxycholate
-
dose-dependent inhibition
Tetcyclacis
-
-
Triton X-100
-
dose-dependent inhibition
Triton X-114
-
dose-dependent inhibition
Tween 20
-
dose-dependent inhibition
Tween 80
-
dose-dependent inhibition
Zwittergent
-
dose-dependent inhibition
-
Zwittergent 3-08
-
dose-dependent inhibition
Zwittergent 3-10
-
dose-dependent inhibition
-
Zwittergent 3-12
-
dose-dependent inhibition
Zwittergent 3-14
-
dose-dependent inhibition
Zwittergent 3-16
-
dose-dependent inhibition
additional information
-
a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase inhibits the flavonoid 3',5'-hydroxylase. No effect by diethylpyrocarbonate or phenylmethylsulfonyl fluoride
-