1.14.14.81: flavanoid 3',5'-hydroxylase

This is an abbreviated version!
For detailed information about flavanoid 3',5'-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.81

1.14.14.81

anthocyanins

3\'-hydroxylase

delphinidin

flavonols

dihydroflavonols

4-reductase

berry

petunia

cyanidin

b-ring

delphinidin-based

pelargonidin

gentian

leucoanthocyanidins

3',5'-hydroxylated

malvidin

3-o-glucosyltransferase

dihydrokaempferol

flavan-3-ols

cyp75b

petunidin

f3\'hs

co-pigment

nutrition

red-skinned

Reaction

Synonyms

Cyp75A, CYP75A31, CYP75A42, CYP75A47, CYP75A48, cytochrome P450-like flavonoid 3'5'-hydroxylase, DkF3'5'H, EC 1.14.13.88, F3',5'H, F3'5'H, flavonoid 3',5'-hydroxylase, flavonoid 3'5' hydroxylase, flavonoid 3'5'-hydroxylase, More, plant P450 flavonoid 3',5'-hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.81 flavanoid 3',5'-hydroxylase

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Results	in table
4	Activating Compound
137	AA Sequence
5	Application
1	CAS Registry Number
40	Cloned(Commentary)
10	Cofactor
6	Expression
22	General Information
1	General Stability
34	Inhibitors
4	kcat/KM [mM/s]
4	KM Value [mM]
17	Localization
1	Metals/Ions
62	Natural Substrates/ Products (Substrates)
62	Organism
4	Pathway
19	pH Optimum
16	Protein Variants
3	Reaction
56	Reference
82	Source Tissue
3	Specific Activity [micromol/min/mg]
2	Storage Stability
147	Substrates and Products (Substrate)
84	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 1.14.14.81 - flavanoid 3',5'-hydroxylase

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flavanone,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3',5'-dihydroxylating)

A cytochrome P-450 (heme-thiolate) protein found in plants. The 3',5'-dihydroxyflavanone is formed via the 3'-hydroxyflavanone. In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin). The enzyme catalyses the hydroxylation of 5,7,4'-trihydroxyflavanone (naringenin) at either the 3' position to form eriodictyol or at both the 3' and 5' positions to form 5,7,3',4',5'-pentahydroxyflavanone (dihydrotricetin). The enzyme also catalyses the hydroxylation of 3,5,7,3',4'-pentahydroxyflavanone (taxifolin) at the 5' position, forming ampelopsin.