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Literature summary for 1.14.14.81 extracted from

  • Bogs, J.; Ebadi, A.; McDavid, D.; Robinson, S.P.
    Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development (2006), Plant Physiol., 140, 279-291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.81

Application

Application Comment Organism
additional information understanding the regulation of flavonoid hydroxylases could be used to modify flavonoid composition of fruits Vitis vinifera

Cloned(Commentary)

Cloned (Comment) Organism
gene F3',5'H1, DNA and amino acid sequence determination and analysis, phylogenetic tree, developmental expression analysis, comparison of red and white cultivar enzyme expression levels, functional expression in Petunia hybrida altering the hosts' flower color and flavonoid composition Vitis vinifera

Protein Variants

Protein Variants Comment Organism
additional information transgenic Petunia hybrida lines, overexpressing the enzyme from Vitis vinifera, produce high levels of the 3',4',5'-hydroxylated anthocyanin malvidin and very little 3',4'-hydroxylated anthocyanins, they also show a shift from kaempferol to quercetin when compared to the nontransgenic control, flower colours and phenotypes, white and red grape varieties exhibit different temporal expression of F3',5'H1 and cytochrome b5, overview Vitis vinifera

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Vitis vinifera
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Vitis vinifera
-
 dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information Vitis vinifera transcriptional regulation of the enzyme in fruits, the enzyme is expressed after flowering, when proanthocyanidins are synthesized, accumulation of hydroxylated anthocyaniins in grape berries, flavonoid biosynthetic pathways, overview ?
-
 ?
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Vitis vinifera
-
 (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Vitis vinifera Q2UYU7 cv. Shiraz, gene F3',5'H1
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit berry skin, transcriptional regulation of the enzyme, onset of expression after flowering, low expression at the onset of ripening, high expression after veraison concomitant with the accumulation of 3'- and 3',5'-hydroxylated anthocyanins, overview Vitis vinifera
-
additional information no expression in seeds Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Vitis vinifera dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information transcriptional regulation of the enzyme in fruits, the enzyme is expressed after flowering, when proanthocyanidins are synthesized, accumulation of hydroxylated anthocyaniins in grape berries, flavonoid biosynthetic pathways, overview Vitis vinifera ?
-
 ?
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Vitis vinifera (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
F3',5'H
-
 Vitis vinifera

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 expression of cytchrome b5 in grape berries is correlated to enzyme expression during fruit development phases, overview Vitis vinifera