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0.00015
-
+/-0.00001, S17-1/pPT4, with IPTG
0.00017
-
+/-0.00002, S17-1/pMMB66EH and S17-1/pPT4 without IPTG
0.00022
-
+/-0.00001, S17-1/pMMB66EH, with IPTG
0.00034
-
+/-0.00006, S17-1/pPT3, without IPTG
0.0004
-
+/-0.00008, PAO1/pMMB66EH, mid-exponential, without IPTG
0.00056
-
+/-0.00024, PAO1/pMMB66EH, early-stationary, with IPTG
0.00076
-
+/-0.00016, FRD2/pMMB66EH, mid-exponential, with IPTG
0.00089
-
+/-0.0002, FRD2/pMMB66EH, mid-exponential, without IPTG
0.00091
-
+/-0.00042, PAO1/pMMB66EH, mid-exponential, with IPTG
0.00094
-
+/-0.00052, FRD2/pMMB66EH, early-stationary, with IPTG
0.00106
-
+/-0.00062, PAO1/pMMB66EH, early-stationary, without IPTG
0.0013
-
+/-0.00004, FRD2/pMMB66EH, early-stationary, without IPTG
0.002
-
inorganic phosphate limitation, 85 h after changing carbon source from sorbitol to sucrose
0.002 - 0.015
-
mucoid forms, activity correlated with alginate production and level of transcription of algD gene
0.0025
-
oxygen limitation
0.0058
-
+/-0.002, 8821/pMMB24, not IPTG induced
0.00685
-
+/-0.00228, FRD1/pMMB66EH, mid-exponential, with IPTG
0.00749
-
+/-0.00187, PAO1/pPT3, mid-exponential, without IPTG
0.00936
-
+/-0.00063, PAO1/pPT3, early-stationary, without IPTG
0.00968
-
+/-0.00155, FRD1/pMMB66EH, mid-exponential, without IPTG
0.00992
-
+/-0.00105, PAO568/pMMB66EH, mid-exponential, without IPTG
0.01097
-
+/-0.00195, FRD2/pPT4, early-stationary, with IPTG
0.01232
-
+/-0.00397, PAO568/pMMB66EH, mid-exponential, with IPTG
0.01249
-
+/-0.00113, FRD2/pPT4, early-stationary, without IPTG
0.0128
-
+/-0.0018, 8821/pVD211, not IPTG induced
0.01283
-
+/-0.00348, FRD2/pPT3, early-stationary, without IPTG
0.013
-
+/-0.003, PAO1/pPT3, without IPTG
0.01312
-
+/-0.0031, FRD2/pPT3, mid-exponential, without IPTG
0.014
-
+/-0.0014, PAO1/pPT4, with IPTG
0.01401
-
+/-0.00147, PAO1/pPT4, early-stationary, with IPTG
0.01436
-
+/-0.00303, FRD1/pPT3, mid-exponential, without IPTG
0.0147
-
+/-0.0014, PAO1/pPT4, without IPTG
0.01472
-
+/-0.00135, PAO1/pPT4, early-stationary, without IPTG
0.0176
-
+/-0.0009, PAO568/pMMB66EH, with IPTG
0.0182
-
+/-0.0003, PAO568/pMMB66EH, without IPTG
0.01886
-
+/-0.00526, PAO568/pMMB66EH, early-stationary, without IPTG
0.01891
-
+/-0.0001, PAO568/pPT3, mid-exponential, without IPTG
0.0223
-
+/-0.0011, PAO568/pPT3, without IPTG
0.0226
-
+/-0.0025, 8821/pVD211, induced by 0.5 mM IPTG
0.02322
-
+/-0.00432, PAO568/pMMB66EH, early-stationary, with IPTG
0.02418
-
+/-0.00628, FRD1/pMMB66EH, early-stationary, without IPTG
0.0251
-
+/-0.0027, PAO568/pPT4, without IPTG
0.02514
-
+/-0.00274, PAO568/pPT4, early-stationary, without IPTG
0.0254
-
+/-0.004, PAO568/pPT4, with IPTG
0.02542
-
+/-0.00139, PAO568/pPT4, early-stationary, with IPTG
0.02574
-
+/-0.00581, FRD1/pMMB66EH, early-stationary, with IPTG
0.0265
-
+/-0.003, 8821/pVD211, induced by 3 mM IPTG
0.02671
-
+/-0.00459, FRD1/pPT4, early-stationary, with IPTG
0.028
-
+/-0.0029, 8821M/pMMB24, not IPTG induced
0.02905
-
+/-0.00427, FRD1/pPT4, early-stationary, without IPTG
0.02994
-
+/-0.0066, PAO568/pPT3, early-stationary, without IPTG
0.03208
-
+/-0.00629, FRD1/pPT3, early-stationary, without IPTG
0.0579
-
+/-0.0029, 8821/pVD211, not IPTG induced
0.084
-
+/-0.0019, 8821/pVD211, induced by 0.5 mM IPTG
0.0942
-
+/-0.004, 8821/pVD211, induced by 3 mM IPTG
0.1259
-
+/-0.02878, PAO1/pPT3, mid-exponential, with IPTG
0.1364
-
+/-0.01055, FRD2/pPT3, mid-exponential, with IPTG
0.1627
-
+/-0.03188, PAO568/pPT3, mid-exponential, with IPTG
0.1758
-
+/-0.03971, FRD1/pPT3, mid-exponential, with IPTG
0.1895
-
+/-0.00702, S17-1/pPT3, with IPTG
0.1919
-
+/-0.00268, PAO568/pPT3, early-stationary, with IPTG
0.2037
-
+/-0.06998, PAO1/pPT3, early-stationary, with IPTG
0.2041
-
+/-0.00932, PAO1/pPT3, with IPTG
0.2097
-
+/-0.03274, FRD1/pPT3, early-stationary, with IPTG
0.2196
-
+/-0.05517, FRD2/pPT3, early-stationary, with IPTG
0.2378
-
+/-0.0552, PAO568/pPT3, with IPTG
0.0009
-
+/-0.0003, PAO1/pMMB66EH, without IPTG
0.0009
-
+/-0.0002, PAO1/pMMB66EH, with IPTG
additional information
-
-
additional information
-
increase of activity has only limited effect on the stimulation of alginate synthesis, existance of other rate-limiting enzymatic step suggested
additional information
-
presence of 1 mM GDP-mannose, GDP-glucose, GMP, GDP, GTP or cGMP retards tryptic digest of enzyme and protects subsequent loss of enzyme activity, amino-terminal domain contains substrate and cofactor binding sites, carboxy-terminal domain is essential for catalytic activity
additional information
-
higher enzyme activities after IPTG induction only for pPT3 containing strains, low GDP-mannuronate production with pMMB66EH only detectable in mucoid strains, higher with pPT3 with increase in cells of mucoid and non-mucoid strains with IPTG, alginate formation occurs only in mucoid strains without significant increase with IPTG, later enzyme of alginate biosynthesis suggested to become rate-limiting